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OfflineFraggin
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First Open Air inoculation. First Contams.
    #6226899 - 10/30/06 02:34 PM (17 years, 4 months ago)

Well, after seeing so many topics of how open air innoculations are possible without contams, I felt brave enough to give it a go.
Well, out of 9 Grain Jars, I have 7 contams.
Though using a glove box requires a bit of patience and dexterity, I have never gotten a contam while using it. I hate to blame it all on open air inoculation, but maybe I just have a dirty air in my kitchen. Gives me some insight as to why bread goes moldy pretty quickly in my house.

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OfflineRogerRabbitM
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Re: First Open Air inoculation. First Contams. [Re: Fraggin]
    #6227186 - 10/30/06 03:52 PM (17 years, 4 months ago)

Anyone who recommends open air inoculations can be compared to a drunk who recommends drinking and driving.
RR


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Invisiblemonstermitch
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Re: First Open Air inoculation. First Contams. [Re: RogerRabbit]
    #6227212 - 10/30/06 03:57 PM (17 years, 4 months ago)

Quote:

RogerRabbit said:
Anyone who recommends open air inoculations can be compared to a drunk who recommends drinking and driving.
RR




that's a bit harsh.
http://www.shroomery.org/forums/showflat.php/Number/5296250#Post5296250
it can be done correctly in my experience.
and many others here have great experience with it.

maybe I'm misunderstanding the "open air" definition.


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OfflineRogerRabbitM
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Re: First Open Air inoculation. First Contams. [Re: monstermitch]
    #6227324 - 10/30/06 04:18 PM (17 years, 4 months ago)

Agar is a very experienced grower. His method you linked keeps the needle covered at all times in an alcohol soaked cloth. That's actually not 'open air'. Leave the needle in open air and see what happens. A glovebox is very easy to make.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleBIGSWANG
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Re: First Open Air inoculation. First Contams. [Re: RogerRabbit]
    #6227335 - 10/30/06 04:20 PM (17 years, 4 months ago)

Quote:

RogerRabbit said:
Anyone who recommends open air inoculations can be compared to a drunk who recommends drinking and driving.
RR




lol..i agree with mm..that is a bit harsh..

i def recommend using a glove box or a flow hood if you have one available...but you can have success without...Ive had success doing some wacky stuff in the past..just cause it worked doesn't mean it will work like that every time...

IMO using every tool and technique available to keep contams out and away from your work area is always the best plan of action....obviously..lol


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InvisiblePigsarefood
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Registered: 06/11/06
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Re: First Open Air inoculation. First Contams. [Re: BIGSWANG]
    #6227347 - 10/30/06 04:22 PM (17 years, 4 months ago)

weird, ive done 9 pf open air innoculations with no contam at all.

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OfflinePr0_X
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Re: First Open Air inoculation. First Contams. [Re: Pigsarefood]
    #6227388 - 10/30/06 04:31 PM (17 years, 4 months ago)

All my innoc's are open air...

I flame sterilize the needle and then wipe it down with peroxcide and quickly innoc..... Ive had jars contam, but 9 out of 10 times the contam's were either because of poor sterilization procedures (my old pc sucked!) or they entered in through the tinfoil lid (since i didnt use a dry verm layer and tinfoil makes a shitty lid) or they got in when I prepared the syringe (only happened once) I just did 8 500ml rye jar innoc's in open air i'll be posting results shortly :smile:


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InvisibleDihnekis
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Re: First Open Air inoculation. First Contams. [Re: Pr0_X]
    #6227489 - 10/30/06 04:58 PM (17 years, 4 months ago)

I'm 6/6 for open air... but I still made a glove box for my next 9 jars. No sense risking it, and I made mine a cardboard box, a piece of plastic, and some duct tape.

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InvisibleBlutjager
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Re: First Open Air inoculation. First Contams. [Re: Dihnekis]
    #6227522 - 10/30/06 05:08 PM (17 years, 4 months ago)

No matter how much I clean the room I inoculate in everything I have ever done out of a glove box was green in a matter of days,If just must be where I live or something cause its not like my house is a pigstye or anything as a matter of fact its quite clean,thats the bitch though no matter how clean it looks and smells you never know what microscopic nasties are floating around,The drunk driving analogy may sound a bit harsh so I will give up another one how about people who can inoculate in open air should go and spend all their money on lottery tickets cause they sure are lucky

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Invisibleagar
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Re: First Open Air inoculation. First Contams. [Re: Fraggin]
    #6227545 - 10/30/06 05:14 PM (17 years, 4 months ago)

Wrapped like a condom (in an alc soaked pad, cloth or gauze) is not open air.

Plus, if you attempt it, it should be done is a clean/sanitized still air environment, and with a surgical grade mask & hair covering on.

Open air inoculation odds are about like having unprotected intercourse with an old crack whore, who services 50 customers a night in an alley on the other side of the tracks in the darkest part of the ghetto.

Odds are extremely HIGH, you will get more than you bargain for.  :eek: :mad2:


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InvisibleHelltick
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Re: First Open Air inoculation. First Contams. [Re: RogerRabbit]
    #6227613 - 10/30/06 05:34 PM (17 years, 4 months ago)

What about G2G transfers...using one quart jar to knock up twenty.
How can one possibly do this in a glove box?


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InvisibleBIGSWANG
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Re: First Open Air inoculation. First Contams. [Re: Helltick]
    #6227631 - 10/30/06 05:39 PM (17 years, 4 months ago)

i do it in a glove box all the time...if you have to, open it after inculating a few jars..and restart the process...

a better idea is to build a little chamber to pass jars through while working...i use a large quaker oat canister wrapped in duct tape with two lids on each side..that way i can get the spawned jars out..and the new jars in without creating to much air flow inside the glove box..


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InvisibleHelltick
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Re: First Open Air inoculation. First Contams. [Re: BIGSWANG]
    #6227646 - 10/30/06 05:44 PM (17 years, 4 months ago)

hmm...good idea!
Thanks bigswang.
:thumbup:


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Invisiblemonstermitch
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Re: First Open Air inoculation. First Contams. [Re: RogerRabbit]
    #6227861 - 10/30/06 06:32 PM (17 years, 4 months ago)

Quote:

RogerRabbit said:
Agar is a very experienced grower. His method you linked keeps the needle covered at all times in an alcohol soaked cloth. That's actually not 'open air'. Leave the needle in open air and see what happens. A glovebox is very easy to make.
RR




that's what I thought.
I use a method very, very similar to agar's and Hippie Chick's.
the needle never, ever touches open air.

I agree with you, any open air touching a needle is terrible news.

got it, thanks.


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Offlinecubeladd
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Re: First Open Air inoculation. First Contams. [Re: monstermitch]
    #6227958 - 10/30/06 06:57 PM (17 years, 4 months ago)

I don't really understand the whole glovebox thing, how is the air inside any more sterlile than the air outside?


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InvisibleSaturnGlass
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Registered: 05/12/06
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Re: First Open Air inoculation. First Contams. [Re: cubeladd]
    #6228068 - 10/30/06 07:21 PM (17 years, 4 months ago)

You clean the air inside :wink:

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InvisibleBlutjager
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Re: First Open Air inoculation. First Contams. [Re: cubeladd]
    #6228138 - 10/30/06 07:32 PM (17 years, 4 months ago)

Quote:

cubeladd said:
I don't really understand the whole glovebox thing, how is the air inside any more sterlile than the air outside?



You spray everything in there down with a bleach solution and because its still air nothing bad gets in your jars

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OfflineRogerRabbitM
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Re: First Open Air inoculation. First Contams. [Re: SaturnGlass]
    #6228203 - 10/30/06 07:42 PM (17 years, 4 months ago)

Gloveboxes are not sterile. The air inside a glovebox is still. By having still air, the contaminant spores that are there don't get blown in by natural room circulation which is what happens in open air.

It helps to mist the air within the glovebox. The mist will tend to collect spores in the air and drop them to the floor of the glovebox. Don't use Lysol or oust if you plan to use a flame to sterilize needles or scalpels, etc.

Grain to grain transfers are much easier with a flowhood, but a tall rubbermaid makes a glovebox big enough to pour from one jar into the other. I wouldn't recommend doing 20 to 1 though. 10 to 1 is a good ratio.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinetonyperez420
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Re: First Open Air inoculation. First Contams. [Re: RogerRabbit]
    #6228349 - 10/30/06 08:13 PM (17 years, 4 months ago)

I work under a FH

In my dirty city, I do not think open air is possible... but I have never tried it.
IMO location is important

I think I would have a better chance going raw dawg on that crack whore agar speaks of

:hehehe:

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OfflineGrogan
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Re: First Open Air inoculation. First Contams. [Re: tonyperez420]
    #6228577 - 10/30/06 08:51 PM (17 years, 4 months ago)

blutjager, make sure alllll windows all air conditioning, all heat, any thing blowing... pc's if near.. are off... I wear a mask, soak the hell out of my hands with alc flame sterlize needle then wrap with alco soaked towel keep it soaked until the needle is through poly fill, then when removed dap polyfill with alc towel..


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