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Registered: 02/28/02
Posts: 36
Loc: SinCity
Media Antibiotics
    #622241 - 04/29/02 12:32 PM (21 years, 11 months ago)

Has anyone experimented with adding antibiotics to the substrate? I know that sporeworks sells something but I'm not sure what it is exactly. A friend has a bunch of boxes of 50cc bottles of liquid Gentamicin. Is there a formula for calcuating the effective dose for use in agar or substrate? Any data would be much appreciated!

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Registered: 11/03/01
Posts: 169
Re: Media Antibiotics [Re: BeautifulDay]
    #622294 - 04/29/02 02:05 PM (21 years, 11 months ago)

50-100mg/L is the recommended concentration of gentamicin sulfate.

- Armedia

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Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
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Re: Media Antibiotics [Re: BeautifulDay]
    #623062 - 04/30/02 09:58 AM (21 years, 11 months ago)

I use gentamicin sulfate in most of my agar solutions, but I try to limit its use as much as possible. I am not an expert, but I would not recommend using it with a substrate. I would be worried about growing super-bacteria that becomes immune to the antibiotic. Instead, I would improve my sterilization techniques.

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