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OfflineGrogan
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LC and H202
    #6185630 - 10/19/06 09:35 AM (17 years, 4 months ago)

how much H2O2 is safe (what ratio) and what concentrate would you reccomend?


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OfflineRogerRabbitM
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Re: LC and H202 [Re: Grogan]
    #6185645 - 10/19/06 09:40 AM (17 years, 4 months ago)

Zero.
RR


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OfflineNewbieS
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Re: LC and H202 [Re: RogerRabbit]
    #6185737 - 10/19/06 10:10 AM (17 years, 4 months ago)

Yeah once you PC or steam sterilize the honeywater, it's contam free.  Just peel back foil, inject, and incubate :grin:

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Invisiblefunnyfeeling
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Re: LC and H202 [Re: Grogan]
    #6186070 - 10/19/06 11:42 AM (17 years, 4 months ago)

If you are worried about contams, I'd go with a dual layer of foil on top of all my LC's. I mark the hole I use for inoculation with a black marker on both layers of the foil, peel back only one layer and shoot through the bottom layer. Haven't had any contams yet.

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OfflineGrogan
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Re: LC and H202 [Re: funnyfeeling]
    #6187587 - 10/19/06 07:07 PM (17 years, 4 months ago)

Well i mean if a jar looks contam will h202 kill off bacterial contams?


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"Maybe a cow occasionally ate a shroom, but it certainly wouldn't be such a potent shroom that the cow would be trippin balls. " LOL

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Invisiblecreamcorn
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Re: LC and H202 [Re: Grogan]
    #6187602 - 10/19/06 07:12 PM (17 years, 4 months ago)

h2o2 doesnt belong in an LC.

if your jar looks contamed its time to throw it out and start again, its too late. h2o2 will not save it, and will only serve to harm the fragile bits of mycelium in a healthy culture.

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Offlinefaceyneck
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Re: LC and H202 [Re: Grogan]
    #6187612 - 10/19/06 07:17 PM (17 years, 4 months ago)

Quote:

Grogan said:
Well i mean if a jar looks contam will h202 kill off bacterial contams?




Better. It'll kill the mycelium too!

Seriously, though, I don't know whether or not there is an amount of h202 that you could add that would kill the bacteria and not the mycelium. I would suggest that if it is contaminated to start over.


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Offlineunkempt
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Re: LC and H202 [Re: faceyneck]
    #6187636 - 10/19/06 07:24 PM (17 years, 4 months ago)

ide say if its contaminated and u wanna try it out why not? its already screwed and its not like your going to waste a full bottle (at least i hope not)

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OfflineGrogan
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Re: LC and H202 [Re: unkempt]
    #6187759 - 10/19/06 08:01 PM (17 years, 4 months ago)

Well from PF's site I read that it will probally not kill any germinated bacteria or mold.. but endospores or any other un-germinateds will be toasted.. and myc can and will grow in H202 Check out  http://www.fanaticus.com/peroxide.htm

(at least according to this hehe)
So i guess... it would be useless to put it in unless I clone or innoc with myc thats already going :blush:


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"Maybe a cow occasionally ate a shroom, but it certainly wouldn't be such a potent shroom that the cow would be trippin balls. " LOL

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InvisibleHolydiver
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Re: LC and H202 [Re: Grogan]
    #6187785 - 10/19/06 08:05 PM (17 years, 4 months ago)

H202 really has no value for this hobby, especially in an LC.  Don't bother.  It doesn't even touch cobweb mold, which is where it got a false foothold around here, somehow.

Do a proper sterilization and work on your sterile procedures, energy/time much better spent.  :thumbup:


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Offlinehyphae
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Re: LC and H202 [Re: Grogan]
    #6187798 - 10/19/06 08:10 PM (17 years, 4 months ago)

Bottom line contamed LC's need to be tossed no questions. As I've said before cloning can be done with h2o2 and LC so you know this is one case in which they can certainly be combined not for the purpose of the additional added h2o2 but for the sake that it won't hurt a thing when dumping your cloned tissue/h2o2 into the LC. Separate issue I know but FYI.


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OfflineGrogan
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Re: LC and H202 [Re: Holydiver]
    #6187809 - 10/19/06 08:13 PM (17 years, 4 months ago)

Sounds good to me :smile:
First step.. Need to get a good glove box built!!
I wear a mask already, wash, and sterilize hands with alcohol, clean surfaces with tilex, then lysol.. clean needles knifes with alcohol ..
Keep all fans off and allow no drafts in.. Have some procedures down just need a glove box built lol..


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"Maybe a cow occasionally ate a shroom, but it certainly wouldn't be such a potent shroom that the cow would be trippin balls. " LOL

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InvisibleHolydiver
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Re: LC and H202 [Re: Grogan]
    #6187821 - 10/19/06 08:16 PM (17 years, 4 months ago)

Before I eat my words: As hyphae mentioned H202 can be useful when cleaning tissue for cultures, but that's about the only use you'll see for it. Good luck.

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OfflineRogerRabbitM
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Re: LC and H202 [Re: Holydiver]
    #6187914 - 10/19/06 08:34 PM (17 years, 4 months ago)

Besides that it melts cobweb on contact on casing layers.
RR


--------------------
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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineelviemaynard
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Re: LC and H202 [Re: Grogan]
    #6188429 - 10/19/06 10:33 PM (17 years, 4 months ago)

I know that there is a tek that says to wait a day and add h2o2 but dont do it. I did it and my jars did jack but I am having LC problems all the way around right now.


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Hey! give me a break I am just starting this crap.

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