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OfflineIgnatius
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Registered: 09/12/06
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Loc: Prague, Czech Republic
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Fact or urban legend re: philosopher stones from Amsterdam?
    #6185162 - 10/19/06 07:28 AM (14 years, 6 months ago)

I've read a few posts by people proclaiming that sclerotia aka Magic Truffles or philosopher stones in Amsterdam contain a specially added bacteria which prevents cloning.  I can understand the economics - by preventing cloning users are encouraged to buy the pricey Mexicana spores, but seriously, this sounds like a big boatload of crap.  How could bacteria inhibit growth in an lc when they couldn't inhibit in a substrate? Until one of the big dogs on this board confirms or denies I'll give it a go. 

So here they are are, straight from A'dam -- 221 grams of love in the pic  :thumbup: and I would like to know - "to clone or not to clone".



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OfflineRogerRabbitM
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Re: Fact or urban legend re: philosopher stones from Amsterdam? [Re: Ignatius]
    #6185172 - 10/19/06 07:39 AM (14 years, 6 months ago)

Urban myth I guess. I never heard it. Clone away. Bacteria is always a problem with cloning any fungi, regardless of species. Use antibiotic agar and wash the cultures with iodine before placing on the agar.
RR


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OfflineIgnatius
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Registered: 09/12/06
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Loc: Prague, Czech Republic
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Re: Fact or urban legend re: philosopher stones from Amsterdam? [Re: RogerRabbit]
    #6185210 - 10/19/06 08:11 AM (14 years, 6 months ago)

Thanks RR. So help me out. I've got this dreadful looking stone in my hand. I'm supposed to slice it open (in my glovebox), remove the meaty white tissue in the middle, and clean it a bit in H2O2?


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OfflineRogerRabbitM
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Re: Fact or urban legend re: philosopher stones from Amsterdam? [Re: Ignatius]
    #6185773 - 10/19/06 12:18 PM (14 years, 6 months ago)

No. With gloved hands, break it in half, don't slice. Slicing with a knife carries contaminants from the surface into the middle. With the virgin territory exposed in the middle of the stone, use a razor sharp scalpel blade to cut a tiny piece out of the center of the stone no larger than a grain of rice. Place this on agar. Repeat several times for each dish. The more clones you take, the better your chances of one of them taking off. Iodine will help to clean the outside of the stone before you start work, but don't use any on the piece you transfer, and don't use peroxide either.

As soon as you see growth, transfer it away from the original petri dish to a new one. If you see several growths, transfer each to a new dish to grow out. This way, if mold or bacteria begins to grow, it will get isolated in it's own petri dish to be identified and tossed out later. Hopefully, one of your dishes will have mushroom mycelium growing from the stone. Good luck.
RR


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semper in excretia sumus solim profundum variat

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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