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Offlinevinzy_a
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after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) *DELETED*
    #6177707 - 10/17/06 07:58 AM (17 years, 5 months ago)

Post deleted by vinzy_a

Reason for deletion: a


Edited by vinzy_a (10/17/06 08:31 AM)

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Offlinevinzy_a
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: vinzy_a]
    #6178004 - 10/17/06 10:06 AM (17 years, 5 months ago)

ummm.. help please.. should i shake that popcorn of pan.cyan. myc? can i still use that? is it contaminated?

thanks a billion

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OfflineRogerRabbitM
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: vinzy_a]
    #6178011 - 10/17/06 10:13 AM (17 years, 5 months ago)

I recognize that pan cyan popcorn container as having bacillus, which is the 'wet spot bacteria'. Popcorn is horrible for harboring heat resistant bacterial endospores and should not be used for mycology when ANYthing else is available. It needs to be tossed out along with the green/black mold ones.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinevinzy_a
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: RogerRabbit]
    #6178014 - 10/17/06 10:15 AM (17 years, 5 months ago)

:frown:  will do

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OfflineRogerRabbitM
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: vinzy_a]
    #6178016 - 10/17/06 10:16 AM (17 years, 5 months ago)

You also don't need your agar to be thicker than 1/8". It will go a lot further that way. A half liter bottle of agar is enough for a sleeve of 20 petri dishes.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinevinzy_a
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: RogerRabbit]
    #6178019 - 10/17/06 10:19 AM (17 years, 5 months ago)

thanks.. i have two of the original petri dishes in the fridge.. i just tried those out on the plastic containers before i left..

thanks RR

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Offline5t3v3
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: vinzy_a]
    #6178023 - 10/17/06 10:20 AM (17 years, 5 months ago)

its improper forum etiquete to bump your post without giving it at least 24 hours for a response. You can shake it, but i think its contaminated. Ive never worked with popcorn tho. Both those look nasty. use that cardboard technique on the last of your ecuadorian syringe. If its the syringe, the contaminants wont grow on the cardboard.

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Offlinevinzy_a
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: 5t3v3]
    #6178025 - 10/17/06 10:21 AM (17 years, 5 months ago)

Quote:

5t3v3 said:
its improper forum etiquete to bump your post without giving it at least 24 hours for a response. You can shake it, but i think its contaminated. Ive never worked with popcorn tho. Both those look nasty. use that cardboard technique on the last of your ecuadorian syringe. If its the syringe, the contaminants wont grow on the cardboard.




sorry..
thanks il try that..

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OfflineRogerRabbitM
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: vinzy_a]
    #6178033 - 10/17/06 10:27 AM (17 years, 5 months ago)

Cardboard is 'ok' for cultures and clones, but spores will do poorly on it. If bacteria is in your syringe, you can use antibiotic agar to isolate away from the bacillus. Chances are, it was just the popcorn which in my opinion is not suitable for use as spawn. Rye or WBS are both far superior.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinevinzy_a
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Re: after 11 days - myc, contam, more myc (pan.cyan, cambo,eq) [Re: RogerRabbit]
    #6178044 - 10/17/06 10:32 AM (17 years, 5 months ago)

yup popcorn was just my test spawn.. to see if the mycelium in the syringe was okay.. i have my other grains here ready.. but i cant find any rye and wbs.. i only have millet and barley..

antibiotic agar.. is that h202 agar?

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