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Offlinepodman19
newbie
Registered: 03/18/02
Posts: 47
Last seen: 22 years, 1 month
question
    #616510 - 04/23/02 03:25 PM (22 years, 1 month ago)

could sterilized jars sit around for a few days if they are unopened and still be sterile? i sterilized mine 5 days ago and cant inoculate until tonight? is it likely that my jars survived and are still contaminant-free??

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OfflineTheShroomHermit
Divine Hermit of the Everything
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Registered: 02/19/02
Posts: 7,575
Loc: border of Canada and Mexi...
Last seen: 1 year, 5 days
Re: question [Re: podman19]
    #616522 - 04/23/02 03:39 PM (22 years, 1 month ago)

They should be, I've always thought that sterilized jars stay sterilized.... I mean, unless you buy into stuff like spontanious germination theories or alchemy...

But alas, i've heard it's not always this way... and that you can get a build up. You should innoculate soon in case there is a contam... the spores can fight it, sorta.

Edited by TheShroomHermit (04/23/02 04:01 PM)

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Offlinepodman19
newbie
Registered: 03/18/02
Posts: 47
Last seen: 22 years, 1 month
Re: question [Re: TheShroomHermit]
    #616531 - 04/23/02 03:52 PM (22 years, 1 month ago)

thanks hermit! one question though? when i'm sterilizing my syringe using alcohol, do i have to burn the alcohol off? willthe alcohol on the needle kill the spores as their coming out?

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OfflineTheShroomHermit
Divine Hermit of the Everything
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Registered: 02/19/02
Posts: 7,575
Loc: border of Canada and Mexi...
Last seen: 1 year, 5 days
Re: question [Re: podman19]
    #616542 - 04/23/02 04:05 PM (22 years, 1 month ago)

Remeber the death sequence on thrax in osmosis jones? Yes, alcohol kills germs, so does heat. Thats why I dip my syringe into the flamming alcohol to get all the germs dead, then u heat it up untill it's read just to be sure.... Flamming should be sufficient. Just alcohol might be ok, i think alcohol does kill spores though, but it's not like it's going to kill all of them...

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OfflineIowa Rasta
enthusiast

Registered: 12/07/00
Posts: 164
Last seen: 20 years, 9 months
Re: question [Re: podman19]
    #616551 - 04/23/02 04:11 PM (22 years, 1 month ago)

dont even worry about it alcohol evaporates really quick so its no problem what so ever


--------------------
http://go.to/freesporering FSR all the way
"Crobih is my Founder of the Shroomery"



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Offlinein_control
Stranger
Registered: 04/20/02
Posts: 8
Loc: Toronto, Canada
Last seen: 22 years, 1 month
Re: question [Re: Iowa Rasta]
    #616576 - 04/23/02 04:38 PM (22 years, 1 month ago)

i would use alcohol at all. i'm not critisizing but when u inject the needle with alcolhol still on the needle. ur basically putting that alcohol in the substulate, which may be enough to contam it. i'd stick with heating up the needle. it works perfectly, and it's worth teh risk of contamin'

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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: question [Re: in_control]
    #616764 - 04/23/02 08:20 PM (22 years, 1 month ago)

Alcohol will contam the substrate ?
where did you read that at ?

Please inform us of your vast knowledge.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 6 months
Re: question [Re: Roadkill]
    #616779 - 04/23/02 08:33 PM (22 years, 1 month ago)

alcohol contaminating substrate! hahaha, thats a good one. the alcohol would just kill everything and guarentee no contamination, if you put enough in, but i dont think a drop of alcohol on the end of a syringe will do much damage, it will probobly evaporate before it makes it to the jar.


--------------------
blasted holes into night until she bled sunshine

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: question [Re: Alpheratus]
    #616799 - 04/23/02 09:02 PM (22 years, 1 month ago)

Exactly

lolz


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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