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Offlinefrogsheath
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Acid as bacteriacide?
    #616410 - 04/23/02 03:23 PM (15 years, 1 month ago)

This is just an idea --would some kind of acid added to your PF substrate
help prevent conatamination? I was reading that in bogs the water is heavily
laden with tannic acid (and other acids) which make it nature's best embalming fluid.
The dead plant matter can't decompose because the acid kills off bacterial decomposers. Now it seems cowshit would be acidic as well (stomach acids). Could
this be something worth further investigation?


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OfflineSeussA
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Re: Acid as bacteriacide? [Re: frogsheath]
    #616454 - 04/23/02 04:10 PM (15 years, 1 month ago)

This is partly why you add lime to a casing... it helps keep the pH slightly basic which doesn't bother cubensis mycelium, but keeps many common contaminates from growing.


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Offlinefrogsheath
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Re: Acid as bacteriacide? [Re: Seuss]
    #616615 - 04/23/02 07:26 PM (15 years, 1 month ago)

So, is cubensis an acid-loving fungus? In this report on bogs there is mention of certain
fungi which benefit from the high acidity of the habitat.


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Offlinefrogsheath
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Re: Acid as bacteriacide? [Re: Seuss]
    #618623 - 04/25/02 07:36 PM (15 years, 1 month ago)

Excuse me for my ignorance but if the "pH is slightly basic" doesn't that mean it's slighly alkaline?


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Invisiblefuzzysquirelnuts
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Re: Acid as bacteriacide? [Re: frogsheath]
    #618627 - 04/25/02 07:44 PM (15 years, 1 month ago)

it sure does


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Offlinefrogsheath
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Re: Acid as bacteriacide? [Re: fuzzysquirelnuts]
    #618978 - 04/26/02 02:26 AM (15 years, 1 month ago)

That doesn't help much fuzzysquirrelnutz.


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Offlinefrogsheath
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Re: Acid as bacteriacide? [Re: frogsheath]
    #618983 - 04/26/02 02:32 AM (15 years, 1 month ago)

I'm still unclear on the concept of what effect acids may have on a cubensis mycelium.


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Offlinesylo
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Re: Acid as bacteriacide? [Re: frogsheath]
    #619085 - 04/26/02 05:56 AM (15 years, 1 month ago)

Cubensis prefers a medium that is slightly alkaline, and so do bacteria, while most fungi prefer an acid medium. I have found that Cubensis will grow in extremely acid media and, if you get the medium acid enough, you can prevent the growth of most bacteria without seriously affecting the growth of the mycelium. Acid media are also easier to sterilize and the only problems are slower colonization and you still can have mold contams. Some good acidifiers are citric acid, Fruit Fresh (an acid blend used for canning), and tartric acid ( sold as Cream of Tartar for baking purposes). Tartric acid is good because it precipitates out if the medium becomes too acid. I use a quarter tea spoon of acid for one liter of liquid medium or one half to one tsp. for a quart of grain.


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Offlinefrogsheath
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Re: Acid as bacteriacide? [Re: sylo]
    #619326 - 04/26/02 12:11 PM (15 years, 1 month ago)

Thanks for the notes sylo!


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