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OfflineRogerRabbitM
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Oyster Straw Log
    #6141843 - 10/06/06 10:24 PM (17 years, 5 months ago)

This straw log was made from mycelium I cloned earlier this summer from a cluster of oyster mushrooms we picked growing on an Alder tree in the Cascade mountains of central Washington State.

The straw was prepared by chopping into 3" to 4" lengths, and then washing/soaking in a tub of warm water and dish soap. After a two hour soak in warm soapy water, the straw was pasteurized in a rubbermaid tub full of hot water for one hour. The water was maintained between 140F and 160F. One cup of hydrated lime was added to the 10 gallons of pasteurization water.



The cluster on the left is the original source for the clone.


The log was fully colonized after 9 days.


First pins




We got two bowls this size from this straw log. I didn't weigh them, but definitely several pounds. The straw log was 10" in diameter and 36" long.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinewaixingren
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Re: Oyster Straw Log [Re: RogerRabbit]
    #6141905 - 10/06/06 10:48 PM (17 years, 5 months ago)

you make me wish i was a better mycologist.


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Invisibleshobimono
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Re: Oyster Straw Log [Re: waixingren]
    #6142208 - 10/07/06 12:47 AM (17 years, 5 months ago)

Very nice!
Do (did?) you get multiple flushes from the straw log?

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OfflineFreeSporePrints
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Re: Oyster Straw Log [Re: shobimono]
    #6142445 - 10/07/06 03:13 AM (17 years, 5 months ago)

Wonderfull..really :smile:

What was the agar recipe?
Agar to grains?Which grains?
Incubation temps for agar/grain/straw?
Rate of humidity during agar/grains/straw?
Did you use a flow hood for transfers?

and..why the dish soap(and how much)?

Ps. Is it Pleurotus pulmonarius? the color is white and the location is an alder...

Thank you :smile:

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Invisibleohmatic
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Re: Oyster Straw Log [Re: FreeSporePrints]
    #6142452 - 10/07/06 03:20 AM (17 years, 5 months ago)

very nice mate, im curious aswell, soap ?


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

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OfflineHotnuts
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Re: Oyster Straw Log [Re: ohmatic]
    #6142717 - 10/07/06 08:29 AM (17 years, 5 months ago)

I'm guessing it knocks off the waxy coating of the straw quicker? Allowing water to penetrate the straw faster?

Nice project by the way! :thumbup:

Edited by Hotnuts (10/07/06 08:29 AM)

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OfflineRogerRabbitM
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Re: Oyster Straw Log [Re: ohmatic]
    #6142728 - 10/07/06 08:37 AM (17 years, 5 months ago)

Yes soap. I started using liquid antibacterial dish washing soap a couple of years ago and have never looked back. I squirt quite a bit of it into the rubbermaid tub of straw as I'm filling with water. I'd say I use a similar amount to what you'd need to wash a load of dishes that size. Definitely make it foam up from the soap bubbles, and agitate with your hands to wash the straw well in the soapy water.

After the two hour soak, the straw was moved to a new rubbermaid tub with water already at 160F. No rinsing of the soap is done. I just pick up a handful at a time of straw, let it drain, then place in the pasteurization bath.

We filmed the pasteurization and making of this straw log for our instructional video, so you're getting a sneak peak. I still use the same process as described in my straw log tek from a few years ago, except that now I do the initial rinse/soak in soapy water.

Incubation was done at room temperature, which this time of year runs from 65F to 72F. The log was placed in my mini-greenhouse for fruiting, refrigerated with a cooling system for three days at 58F, then increased to room temperature and 95% humidity as the plastic was stripped off the log. Oysters don't usually require a cold shock, but this log went into the greenhouse along with my shaggy manes and shiitake which do, so it had to go along for the ride.

The whole process from building the log until first flush harvest was 20 days. It probably would have been quicker without the cold shock, but that's what happens when you only have room for one greenhouse.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflinePsilocybeingzz
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Re: Oyster Straw Log [Re: RogerRabbit]
    #6142996 - 10/07/06 10:32 AM (17 years, 5 months ago)

No PC at all??

Very cool, but how do you keep the rubbermaid at 160f???
Afer its sealed up does it just stay at that temp for a couple of hours??

I would assume you would have to use a cooler, most rubbermaids I have seen, do not insulate very well.

CHEERS, and great work as always RR. :laugh:


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OfflineRogerRabbitM
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Re: Oyster Straw Log [Re: Psilocybeingzz]
    #6143037 - 10/07/06 10:52 AM (17 years, 5 months ago)

I use two rubbermaid tubs, one inside the other for the insulating effect. After filling, I use both lids, with a towel on top to hold in the heat. During winter when the house is cooler, you can add a pot of hot water from the stove at the halfway point in pasteurization if necessary.

The important thing to remember is to monitor the progress and do whatever it takes under your own environmental conditions to keep the pasteurization bath at 140F to 160F for one hour.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleCorporal Kielbasa

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Re: Oyster Straw Log [Re: waixingren]
    #6143181 - 10/07/06 11:55 AM (17 years, 5 months ago)

Quote:

waixingren said:
you make me wish i was a better mycologist.




Whats so hard about stuffing straw and spawn into a plastic bag?

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InvisibleCorporal Kielbasa

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Re: Oyster Straw Log [Re: RogerRabbit]
    #6143183 - 10/07/06 11:55 AM (17 years, 5 months ago)

Roger nice Shots!

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OfflineOatman2000
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Re: Oyster Straw Log [Re: Corporal Kielbasa]
    #6143239 - 10/07/06 12:28 PM (17 years, 5 months ago)

I like straw logs!
Great job as always!

36" Long!! :eek:
long Log, (say that 3 times real fast!)


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Invisibleohmatic
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Re: Oyster Straw Log [Re: Oatman2000]
    #6143882 - 10/07/06 04:07 PM (17 years, 5 months ago)

commercial oyster logs are rly long too.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

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OfflineCosmicFunGuy
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Re: Oyster Straw Log [Re: ohmatic]
    #6144149 - 10/07/06 05:30 PM (17 years, 5 months ago)

I can't find the straw log tek... :frown: I had it bookmarked at one point, but i still can't find it. All i can find is dead links to it.
help!!!! :hissyfit:

btw... great job as always rr, can't wait for the video...


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♫ ♪ In the garden of many fields, there is no me or you. There is no right or wrong. There is no up or down. There is no black or white. Everyone is the plow man. Everyone is the seed. Everyone harvests and everyone yields. In the garden of many fields, everyone is one with one love and one love is one with everyone. ♪ ♫
lyrics from: Transglobal Underground - Eyeway Souljah (from Psychic Karaoke)

Edited by tickettothemoon (10/07/06 05:34 PM)

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Invisiblemicololo2
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Re: Oyster Straw Log [Re: RogerRabbit]
    #6144244 - 10/07/06 05:54 PM (17 years, 5 months ago)

I've tried this once after I have read this technic, without soap, on Mycotopia forum and it worked well.
So why do you use soap?
You probably don't add supplements like bran, isn't it?
Do you use some kind of nutrition supplement?

For my self I prefer using sawdust-wood chips because:
1. 40 Pd's dry straw cost about 4.50-5$.
2. 40 Pd's dry sawdust in pellets cost 3.80$ and easier to store. Wood chips is free.
3. Pound to pound sawdust-wood chips give more shrooms than straw.
4. In the same time, I prepare a lot more substrates with sawdust than straw.

On the other hand using straw is a low cost of energy. And if there is no supplements is cheaper too. No costly mycobag. To use this technic profitably, will have to find a way to prepare more substrates per hour.

Well done sir, bravo!

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OfflineRogerRabbitM
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Re: Oyster Straw Log [Re: micololo2]
    #6144296 - 10/07/06 06:19 PM (17 years, 5 months ago)

Yes, it's always a trade off. Supplements such as bran need to be sterilized, so they can be used with sawdust, but not straw. Straw colonizes faster than sawdust/chips, so you get the first flush sooner. Sawdust/chips have a more solid substrate so they give more flushes, plus you can supplement with wheat or rice bran for extra performance.

The soap, since it isn't rinsed off seems to help after the pasteurization in warding off molds. I'm not sure why, but perhaps it's the ph or the film left behind.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineHotnuts
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Re: Oyster Straw Log [Re: RogerRabbit]
    #6144491 - 10/07/06 07:31 PM (17 years, 5 months ago)

I'm guessing this isn't an anti-bacterial soap? Something like Ivory exc.?

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OfflineRogerRabbitM
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Re: Oyster Straw Log [Re: Hotnuts]
    #6144622 - 10/07/06 08:20 PM (17 years, 5 months ago)

I use whatever mrs rabbit has sitting by the sink. This time it was Palmolive antibacterial dish soap.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleSemilanceata
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Re: Oyster Straw Log [Re: RogerRabbit]
    #6146271 - 10/08/06 10:51 AM (17 years, 5 months ago)

Don´t you mind about how the soap can affect your health lately? You know, conventional soaps contain edta and unreadeable chemicals that can´t be good at all.
EDIT: They also contain petroleum by-products.


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Sr_Setahongo

Edited by Semilanceata (10/08/06 10:55 AM)

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OfflineRogerRabbitM
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Re: Oyster Straw Log [Re: Semilanceata]
    #6146440 - 10/08/06 12:29 PM (17 years, 5 months ago)

No, mushrooms don't 'soak up' what's in the substrate, they metabolize it. Research has shown that only heavy metals are absorbed by the mycelium. Mushrooms used to clean up toxic petroleum waste sites are edible. Soap is cool. If mycelium transferred what's in the substrate to the fruitbodies we certainly wouldn't be able to grow on manure or compost.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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