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OfflineAlprazolom
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Female

Registered: 09/14/06
Posts: 159
Loc: Manchester
Last seen: 14 years, 4 months
PF jars cooling from PC.. what to do when they're done?
    #6137775 - 10/05/06 11:09 PM (14 years, 9 months ago)

I have some specific questions. First off, 4 1/2 pint jars, 3 of which have 4 holes in the lid (equidistant) and 1 has only 3 holes. The way they are setup is

Jar -> lid -> Tyvek -> ring -> foil. Lids are put upside down (rubber side up) and are not tightly screwed on.

Do I tighten them after they have cooled down?

I am going to poke the needle through the tyvek and through the verm layer and then shoot some spores (EQ). I put 3-4 holes for air exchange, not because I am going to inject through all of the holes. I am thinking of shooting into 1-2 holes and then covering them with durapore tape (3M brand) soaked in alcohol. I am a little low on spore solution so I don't want to inject through all the holes. Maybe .5cc each jar.. is that a problem?

If I do use all the holes, and tape them, is that enough air exchange? Would the durapore tape be a sufficient filter? because that will make the tyvek useless, as it will just be sitting on a metal lid and the holes will be only covered by the tap.

On top of that I'll put 2-3 coffee filters fastened with a rubberband and then toss 'em (not literally) into the incubator which was at 72F this morning, but is significantly higher now, maybe about 80-85F.

I noticed that some people put their jars in the incubator upside down (resting on their lids). Why is this done?


--------------------

My first grow. Thanks all.


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Offlinetrumie04
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Registered: 12/14/05
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Re: PF jars cooling from PC.. what to do when they're done? [Re: Alprazolom]
    #6138080 - 10/06/06 12:20 AM (14 years, 9 months ago)

I am curious as well. bump!


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Offlinethekeeper
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Re: PF jars cooling from PC.. what to do when they're done? [Re: trumie04]
    #6138408 - 10/06/06 01:54 AM (14 years, 9 months ago)

im not an old hand at this...but i think all the tape and tyvek and coffee filters are a little overkill for pf tek ;-)

when using the pf tek, the reason u put the layer of vermiculite on top is to filter contams...so the tape and coffee filters may hinder air exchange...

ppl have jars upside down to move the direction of the colonization if the jars seem to have stalled before completing.

incubation temps look good...

if i have anything wrong, someone correct me.

-shaw


--------------------
everything i said above is fictional....i don't grow illegal mushrooms!

Casing 101

Poor Man s Mycelium Syringe Tek

'-.Always use Oust to sterilize your air.-'


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Offlinethekeeper
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Registered: 07/29/05
Posts: 266
Loc: a country where it is leg...
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Re: PF jars cooling from PC.. what to do when they're done? [Re: thekeeper]
    #6138415 - 10/06/06 01:55 AM (14 years, 9 months ago)

btw..yes tighten the lids asap.

i pc my jars with the lids tight...

-shaw


--------------------
everything i said above is fictional....i don't grow illegal mushrooms!

Casing 101

Poor Man s Mycelium Syringe Tek

'-.Always use Oust to sterilize your air.-'


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Offlineexinth
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Registered: 07/25/06
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Re: PF jars cooling from PC.. what to do when they're done? [Re: thekeeper]
    #6138737 - 10/06/06 02:22 AM (14 years, 9 months ago)

After PCing the only logical thing to do is bury them in your backyard to ripen over the winter. Come spring, crack those babies out and start growin!


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OfflineAlprazolom
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Registered: 09/14/06
Posts: 159
Loc: Manchester
Last seen: 14 years, 4 months
Re: PF jars cooling from PC.. what to do when they're done? [Re: thekeeper]
    #6139053 - 10/06/06 03:11 AM (14 years, 9 months ago)

Thanks. I have 4 jars from an earlier attempt. They are about 5-6 days old and only one is showing signs of growth, and not so much at that.

They were all inoced with EQ, and being that I do see at least SOME growth on one, that rules out the syringe being bad.

Possible problems include air exchange; Lids have 2 holes. A small hole for the needle and a bigger hole full of polyfill. Do they need more air exchange? The lids are on tight and each covered with 2-3 coffee filters. Inoculation hole is sealed w/ masking tape.

The only other thing I can think of is them being too dry, but I'm not sure how I would be able to tell from looking at them, being that this was my first attempt. I do have a hunch that I screwed up while PCing them. How can I check if they are too dry?

Oh and of course, maybe I'm just being impatient  :grin: (/optimism)


--------------------

My first grow. Thanks all.


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