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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Bulk Substrate

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OfflineSacred
Stranger
Registered: 04/01/02
Posts: 16
Last seen: 19 years, 4 months
Innoculation question
    #612026 - 04/18/02 04:56 PM (19 years, 6 months ago)

Hello all, first timer here. Getting ready to innoculate tonight. I'm looking at the
MMGG and PF TEK. Confused about one thing. PF suggests covering the holes in
the top of the jars with heavy duty tape, and then covering that with
aluminum foil before steam sterilzing. Then removing the tape, after the
jars are cool, right before injecting the spore solution. And then recover
the holes with tape, while waiting for colonization - I guess to prevent
contams from entering. MMGG does not say anything about the tape, and says
something along the lines of make sure the holes are in the lid before
steaming or else they could explode. And no mention of covering up the holes
after innoculating. The two methods seem contradictory. If I use tape, I
assume that NO water will exchange while steaming. Without tape, it appears
that some water could potentially evaporate from the substrate and leak out
from underneath the aluminim foil. Also if tape is covering the holes during colonization, how is air going to be exhanged between the jar and the air ? Or is there enough air in there already for the entire 3 week colinization period ? Maybe with the lid looser, air can still get back and forth ? Not looking for a definite answer, as I'm sure everyone has an opinion. Just wanted some thoughts from experienced n00bs. Thanks you guys.


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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,145
Loc: my room
Last seen: 2 days, 16 hours
Re: Innoculation question [Re: Sacred]
    #612036 - 04/18/02 05:02 PM (19 years, 6 months ago)

I put only foil over the holes(lid), during the pressure cooking and colonisation.


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Offlinekb73
enthusiast
Registered: 10/18/01
Posts: 369
Loc: Abilene, TX
Last seen: 11 years, 17 days
Re: Innoculation question [Re: Sacred]
    #612123 - 04/18/02 06:51 PM (19 years, 6 months ago)

I just use foil also. If you cover the holes with tape, the CO2 can't leak out, and your growth will really slow down.


--------------------
WARNING chronicshroom will rip you off! Don't trade with him! I sent him 20 spore syringes and he never sent me anything.


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