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OfflineCosmicFunGuy
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first attempt at agar soon...
    #6116799 - 09/29/06 08:40 PM (17 years, 4 months ago)

I've been growing for a little while now and I'm getting comfortable with the processes of growing from multispore and LC. Now i want to try agar.

I just built my still air/glove box today (forgot this piece though, I will add it tomorrow or Sunday) : IT'S FINISHED NOW
*photo: Roadkill's still air box from the archives*

Here are pics of mine so far (minus the filter):
***thanks to roadkill and monstermitch for excellent teks***
*** Roadkill's still air box & Monstermitch's still air glove box***





So now that i have a glove box I am not going to need antibiotic or peroxided agar, correct? Would peroxided matter for isolating a strain from the wild?

I have read the FAQ and mushroom cultivation archive, thoroughly on the subject of agar. I plan to do searches and read some old posts tonight.

Any input telling me why my glove box will fail(if in fact something is wrong with it) so I can fix it before I use it, will be gladly taken... as well as any more positive feedback. thanks :smile:

EDIT: it's not standing up because i caulked the 4 inch plugs to seal them in and its drying.


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♫ ♪ In the garden of many fields, there is no me or you. There is no right or wrong. There is no up or down. There is no black or white. Everyone is the plow man. Everyone is the seed. Everyone harvests and everyone yields. In the garden of many fields, everyone is one with one love and one love is one with everyone. ♪ ♫
lyrics from: Transglobal Underground - Eyeway Souljah (from Psychic Karaoke)


Edited by tickettothemoon (09/30/06 02:38 PM)


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Offlinefromthemoon
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Re: first attempt at agar soon... [Re: CosmicFunGuy]
    #6117170 - 09/29/06 10:29 PM (17 years, 4 months ago)

Thanks for the edit cause I was a little confused.

I'm a very very newb, but I have yet to have an agar contaim thanks to the glove box.

Best advice,

1) PC your tools in a sealed jar (if you are not flaming)
2) Line the bottom of the box with paper towels soaked with bleach water, spray box/gloves with bleach water (I don't like using lysol, add all you stuff, spary as you seal the box, and let it sit for 30 minutes or over night (the contaims will settle and die when they hit the bleach towels.

I inoculated 9 plates (none had antibiotic or peroxided agar) and none developed contaims (3 didn't grow anything because the syring for those 3 were weak). I had one control plate that I opened first in the box and left open while I inoculated all the others and closed it last; there were no contaim in that either.

Took about 2 to 3 weeks to grow out.

Transfered those to new plates in my glove box, those also took about 2 weeks to grow out; nice roping and thiker myc and again, no contaims.

Transfered the best chunks from those in my glove box and those are still on week one, but no contaims.

Either I'm lucky, sterial, or agar isn't quite as hard as some people think. I see no reason to use antibiotic or peroxided agar.

Glove box looks good to go!


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Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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OfflineCosmicFunGuy
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Re: first attempt at agar soon... [Re: CosmicFunGuy]
    #6118677 - 09/30/06 02:36 PM (17 years, 4 months ago)

<<<MY FINISHED GLOVEBOX>>>



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♫ ♪ In the garden of many fields, there is no me or you. There is no right or wrong. There is no up or down. There is no black or white. Everyone is the plow man. Everyone is the seed. Everyone harvests and everyone yields. In the garden of many fields, everyone is one with one love and one love is one with everyone. ♪ ♫
lyrics from: Transglobal Underground - Eyeway Souljah (from Psychic Karaoke)


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