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Offlinenewmodel
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Registered: 06/24/05
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WBS Stalling (With Pics)
    #6081703 - 09/20/06 10:01 AM (17 years, 4 months ago)

I was not going top post this because there are many post on this already that I have found and read but I have question about a particular strain.

I have about 32 jars of which 24 are fully done (took about 2 weeks). The Strains are Cambodian, South American and F+. The Cambodians are done, and most of the South Americans are done except 2..they stalled. There is about 8% left to colonize, what are my chances of invisible contamination? All the other South American jars look good.

the F+ is what I really want to inquire about. For those that have cultivated this strains, has it proven to be slow. Because while the rest of the jars are 100% colonized (excluding the 2 SA) F+ has just started to colonize...1.5 to 2 weeks in. It's starting to pick-up. But still compared to the rest of the strains this guy is in last place.

Has anyone experienced this? I've been spending some time reading about invisible contaminations, Roger Rabbit spooked me so now I have become paranoid and am contemplating whether or not I should toss some of the jars just to be safe.

I will try to post some pics.

Thanks,

Peace.
I.T.


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A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


Edited by newmodel (09/21/06 03:41 PM)


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OfflineWayward_Son
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Re: WBS Stalling [Re: newmodel]
    #6081927 - 09/20/06 11:13 AM (17 years, 4 months ago)

Not all strains react the same. wbs is okay but maybe not best suited for that strain?. I would guess that since you have so many that are fine, and it is only one strain that is 'left behind' that they are being true to a slower growth rate rather than contams. After two weeks you would be seeing contams in at least one of those jars if that were the case. I don't know for sure but given the amounts of good jars you have and what you say, this is what seems apparent to me. I would not toss jars that show no contams. I am wondering, with so many jars...is their a possibility that the slow to colonise strain is being incubated at a lower temp? It is difficult to have an even temp across all jars in that quantity and a 5 to 10 degree difference can make the difference in speed to colonise. I use a  closet now with a much better heat balance and circulation(than the TiT had) for that reason. Just a thought. You MIGHT have gotten a bad syringe. Much is possible. Only time will tell you what is. You could seperate those jars to a different incubator, and really monitor temp on. Hope it works out whatever you do. :dancing:


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Edited by Wayward_Son (09/20/06 11:22 AM)


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Offlinenewmodel
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Re: WBS Stalling [Re: Wayward_Son]
    #6087160 - 09/21/06 03:35 PM (17 years, 4 months ago)

As promised I took some pictures and am posting them.

To clear some other things up that I have posted, in the heat of the moment, since this post- I will explain a few things that I have done to help you help me.

Now the main problem that I was inquiring and needing your help about was some of my jars have stalled. If to compare the rest of the jars with the stalled ones there are no visible indications of any contaminations or reasons for the stall.

The other questions was regarding a strain; F+ and if anyone knows anything concerning its pace and its desired medium. I am less worried about this as you will see in the pictures.

Finally, I made replied to a post concerning the SMELL of the jars. I know that skunky (or nay bad) smell is not good. I know that I noticed a sour smell from my incubation chamber a while ago. So in order to see if any jars smelled really bad I briefly went over every single one and attempted to determine if any foul order was coming from the jars. None of the jars except the BFR showed any sign of any odor. The BFR's did not have a bad oder to them but had a vague smell that I cannot pin point. However, nothing smelled bad or foul.

Here are the pictures:


Here are the STALLED Jars


This is a close up...the myc. growth almost reminds we of cow web.


The F+ finally started to grow, but as they did they really picked up the speed.


and....some of the 100% colonized jars, so you can compare. All started at the same time, same batch, same amount of love devoted to each one.




Peace,

I.T.


--------------------
A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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Offlineseg_x
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Re: WBS Stalling [Re: newmodel]
    #6087203 - 09/21/06 03:50 PM (17 years, 4 months ago)

have u been shaking the jars?


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Offlinenewmodel
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Re: WBS Stalling [Re: seg_x]
    #6087216 - 09/21/06 03:55 PM (17 years, 4 months ago)

Seg_X~

Shaking the jars:

The stalled ones- have been shook twice. Once at inoculation and once at 25%. For some stalled ones I tried shaking a third time but I did not seem to help.

F+ - I shook once so far and will shake again in the next few days.

Peace,

I.T.


--------------------
A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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Offlinenewmodel
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Re: WBS Stalling [Re: newmodel]
    #6087479 - 09/21/06 05:21 PM (17 years, 4 months ago)

Notce anything unusual about the stalled ones?


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A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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Invisibletealeaf
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Registered: 09/21/06
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Re: WBS Stalling [Re: newmodel]
    #6087517 - 09/21/06 05:30 PM (17 years, 4 months ago)

does the jar on the top left of that stalled ones have red marker on it. if not you definitely have some lipstick contam


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OfflineTweexican
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Re: WBS Stalling [Re: tealeaf]
    #6087590 - 09/21/06 05:49 PM (17 years, 4 months ago)

Most stalling I've endured was the cause of bacterial contam. Smell you rjars in a couple of days.


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OfflineRogerRabbitM
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Re: WBS Stalling [Re: Tweexican]
    #6087679 - 09/21/06 06:12 PM (17 years, 4 months ago)

It looks like bacillus. I would imagine they'll start to smell like rotten apples soon.
RR


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Offlinenewmodel
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Re: WBS Stalling [Re: RogerRabbit]
    #6088063 - 09/21/06 07:57 PM (17 years, 4 months ago)

There is no red, it's the fault of how I took the picture. I checked the smell from 3 hours ago and they still smell fine. However, I took a clsoer look at the stalled jars and it seems cow webish as if the mycelium was dying or loosing color and turning off-white. Maybe it was poo rlighting I'll check on them in a few hours again.

RR I have had them for almost 3 weeks. How can you tell that it is bacillus? Thanks for your help guys.

Peace,

I.T.


--------------------
A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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Offlinemycoguy
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Re: WBS Stalling [Re: newmodel]
    #6089005 - 09/21/06 11:59 PM (17 years, 4 months ago)

turn those jars upside down. trust me, they'll finish as long as there's not too much water.


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InvisibleBlutjager
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Re: WBS Stalling [Re: mycoguy]
    #6089047 - 09/22/06 12:13 AM (17 years, 4 months ago)

Yea you should toss them....Into the back of the closet and forget about them for like a week,Why throw them out now,you have nothing to lose by waiting to see what the do.You might look at them in a week and see nothing but contamination or you may see full colonization or maybe even pins like I just found in a jar I had ignored.You are always it time to throw them out later.I say forget about them for a while and see what happens.Good luck


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Offlinenewmodel
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Re: WBS Stalling [Re: Blutjager]
    #6089640 - 09/22/06 09:23 AM (17 years, 4 months ago)

Thanks a lot for all the advice.

I've been watching they and monitoring odor but I'll seclude them and let them chill and ponder whether they want to be cased with the rest of the mushrooms or miss out.

Bad mycelium...Bad....Bad...

Unless it's my fault, then I'm a bad caretaker.

PEAce,

I.T.


--------------------
A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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Offlinenewmodel
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Registered: 06/24/05
Posts: 418
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Re: WBS Stalling [Re: newmodel]
    #6092981 - 09/23/06 11:34 AM (17 years, 4 months ago)

Some of them are pulling together and seem to be reaching 100% colinizatioon. However, I am skeptical about using them.
I'll post some more pcitures soon.


--------------------
A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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