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 upupup
guardian
Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 10 months
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 PF jar contams twice (now 3) in a row. 
#607320 - 04/13/02 01:44 PM (22 years, 9 months ago) |
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I have had some seriouse trouble with PF jars contaminating all of the sudden and I need some help tracking down what I might be doing wrong. Here is my tek.
All spores collected my myself in a glove box. 2 different strains both times. Syringes made in a glove box. Did the standard PF syringe tek (filled with boiling water 3 times, squirted out, wrapped in tin foil and lightly boiled for an hour). Standard PF jars maximum fruiting formula. Substrate was mixed well then pasturized by steaming for an hour. Jars were innoculated with different syringes to improve my odds. Needles were flame sterilized before each jar. Needle was wiped with alcohol in between holes of the same jar. ALL jars contaminate with the nasty invisible bacteria that STINKS. Help appreciated!
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Support bacteria - they're the only culture some people have.
Edited by upupup (05/14/02 02:53 PM)
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 12 days, 3 hours
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Re: PF jar contams twice in a row. [Re: upupup]
#607811 - 04/14/02 03:25 AM (22 years, 9 months ago) |
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Insufficient sterilisation.
Either use a pressure cooker for 45 minutes, or steam for 90 minutes, or steam 2 days in a raw for 1 hour, 24 hours apart(the so called tyndallization).
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upupup
guardian
Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 10 months
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Re: PF jar contams twice in a row. [Re: Anno]
#614720 - 04/21/02 01:00 PM (22 years, 9 months ago) |
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There is one important variation I have been doing recently that I also wonder if doing is creating the bacteria problem and that is I am leaving my lids less tightly than I used to because I thought it would be better for the hot steam to penetrate better. Now I wonder if there is just too damn much moisture in the jars assisting the anerobic bactera along. Since I am usning the maximum fruiting formula it should have plenty of water already and I should also keep lids tight during pasturization no?
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Support bacteria - they're the only culture some people have.
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baraka
Registered: 07/15/00
Posts: 10,768
Loc: hyperspace
Last seen: 3 years, 21 days
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Re: PF jar contams twice in a row. [Re: upupup]
#614795 - 04/21/02 02:30 PM (22 years, 9 months ago) |
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PF jars never contam for my friend. Boil the fuckers for an hour and do not let water get in. If they are still contaming you must be wiping yer ass with the jar or somthing...
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This is the only time I really feel alive.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 12 days, 3 hours
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Re: PF jar contams twice in a row. [Re: upupup]
#614869 - 04/21/02 04:32 PM (22 years, 9 months ago) |
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Try this tek next time and report the results:
http://www.fungifun.org/pf/pf_en.htm
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upupup
guardian
Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 10 months
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Re: PF jar contams twice in a row. [Re: Anno]
#615041 - 04/21/02 09:04 PM (22 years, 9 months ago) |
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No ass wiping with jars going on here. I am using water from a creek though which is also different.
The link you provided is for a PCer thogh. I no longer have one of those. I am using standard PF pasturization. I have had 100% success with this in the past but all of the sudden two 100% screw ups in a row.
Again, I will ask, is too mush moisture getting in there since I am using maximum fruiting formula and leaving the lids very loose?
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Support bacteria - they're the only culture some people have.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 12 days, 3 hours
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Re: PF jar contams twice in a row. [Re: upupup]
#615250 - 04/22/02 03:47 AM (22 years, 9 months ago) |
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>The link you provided is for a PCer thogh.
Is it?
I read: "If you are unable to find or buy a pressure cooker, you can also sterilize the jars using a big pot with a lid.
In this case steam the jars for 1.5 hours in a pot lid on. Use only approximately 1 inch of water at the bottom.
You might have to add some water to the pot during steaming due to evaporation."
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upupup
guardian
Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 10 months
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Re: PF jar contams twice in a row. [Re: Anno]
#616266 - 04/23/02 10:43 AM (22 years, 9 months ago) |
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Duh. Your right. My bad...
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Support bacteria - they're the only culture some people have.
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upupup
guardian
Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 10 months
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Re: PF jar contams twice in a row. [Re: upupup]
#630635 - 05/14/02 02:52 PM (22 years, 8 months ago) |
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Ok. I followed that tek suggested by anno and all still contaminated. To be specific, I steamed them for 1.5 hours. Really, I am totally confused as to what is going on. It is always the anerobic bacteria. I am saying that it is the bacteria based on the smell. There is no other sign of contam just the smell and the stall. I am somewhat familiar with this bacteria because of my experiences with grain and that bacteria seems to be a prevalent contam for grain.
I am stumped. Steaming is all I can do right now. Could this be becuase of too much moisture? I have asked that question before but it didn't get answered. I am at the end of my rope.
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Support bacteria - they're the only culture some people have.
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 We_come_in_peace
old hand
Registered: 05/13/02
Posts: 762
Loc: Alpha Centauri
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Re: PF jar contams twice in a row. [Re: upupup]
#631172 - 05/15/02 02:51 AM (22 years, 8 months ago) |
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I would invest in a pc. I bought a cheap one at Target Greatland. Granted I can only fit 3 jars in it at one time but I don't get contams anymore. Or if you sick of spending money on home grow stuff just get a few myco bags and a spore syringe from sporeworks.com
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 7 days, 11 hours
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Re: PF jar contams twice in a row. [Re: upupup]
#631275 - 05/15/02 05:14 AM (22 years, 8 months ago) |
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"I am using water from a creek"
Use tap water wich is treated, the water from that creek is one of the variable factors you have to consider, imagine the water is contaminated, how can you predict that ? Also, this time of the year is very prolific is terms of spore counting or microfauna increase, guess where they could end.
Peace,
MAIA
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Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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upupup
guardian
Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 10 months
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Re: PF jar contams twice in a row. [Re: MAIA]
#631362 - 05/15/02 07:25 AM (22 years, 8 months ago) |
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What about my substrate being overly wet? I am really wondering if using the maximum fruiting formula and then so much steam is creating the overly wet conditions perfect for the anerobic bacteria. Any thoughts on that?
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Support bacteria - they're the only culture some people have.
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 7 days, 11 hours
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Re: PF jar contams twice in a row. [Re: upupup]
#632349 - 05/15/02 10:03 PM (22 years, 8 months ago) |
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Damn, you're stubborn . Well then... yes it's the steam that creates too much moisture and anaerobic bacteria grows really fast, happy now ?
Peace,
MAIA
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Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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baraka
Registered: 07/15/00
Posts: 10,768
Loc: hyperspace
Last seen: 3 years, 21 days
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Re: PF jar contams twice in a row. [Re: MAIA]
#632801 - 05/16/02 07:51 AM (22 years, 8 months ago) |
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use tap water. Wrap foil around the jar tops. Boil them for an hour and sit em on a towel. If they are still getting contammed you are introducing them with tainted syringes or somthing. Boiling works 98% with pft tek jars.
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This is the only time I really feel alive.
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Snobrdr311
outdoorenthusiast
Registered: 09/03/01
Posts: 1,468
Loc: Midwest
Last seen: 11 years, 3 months
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Re: PF jar contams twice in a row. [Re: baraka]
#645124 - 05/25/02 06:19 PM (22 years, 8 months ago) |
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it has to be the creek water you use... why on earth would you want to use untreated water, from nature, of which you have no clue the level of bacteria? dude, use common sense and work with bottled water, or even tap water which has worked fine for me..
creek water? my god.. why?
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Anonymous
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 Re: PF jar contams twice (now 3) in a row. [Re: upupup]
#645518 - 05/26/02 04:48 AM (22 years, 8 months ago) |
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I grow the pf style pretty often with little or no mold problems. I always bake my ingredients (i.e. brf, verm) prior to mixing. And i use only sterile water, although alot of ppl use creek water, etc. Also using a pressure cooker is the way to go! Dont be scared to try it, i was kinda worried it would blow up or soemthing but it was fine. It is a must that you sterilize everything you use too...like measuriing cups, bowls, jars, and well everything.........o h yea, if you go get simple green mold you can use a syringe with a 50/50 mix of clorox and water to kill it. Everyday put about 1 cc on the infected area...i usually heat it up too to really give the mold hell! it works, saved 3 jars!!!
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DreaMaTrix
Shaman I am
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
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Re: PF jar contams twice in a row. [Re: upupup]
#645549 - 05/26/02 05:14 AM (22 years, 8 months ago) |
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Maybe U got a contaminated print. Where did U get it from??
Good luck
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"We are the one's we have been waiting for" - Hopi saying
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baraka
Registered: 07/15/00
Posts: 10,768
Loc: hyperspace
Last seen: 3 years, 21 days
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Re: PF jar contams twice in a row. [Re: DreaMaTrix]
#645608 - 05/26/02 06:22 AM (22 years, 8 months ago) |
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red im gonna disagree with you gotta sterilize everything(mixingcontainer, measuring cup, ect...)
MY friend is very sloppy with stuff until he sterilize. No probs.
Just be clean once you boil/pressure cook the jars.
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This is the only time I really feel alive.
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Yowtzie
Stranger
Registered: 08/28/17
Posts: 9
Last seen: 3 years, 2 months
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 Re: PF jar contams twice in a row. [Re: upupup]
#26023063 - 05/30/19 02:12 PM (5 years, 7 months ago) |
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You said you had changed your water source, and was getting it from a creek, perhaps this source has a tough bacteria that needs higher heat, for longer time. A cheap approach - get a ‘water straw’, it filters out over 99% of krap. Trail walkers take them in case they get lost, they can drink from funky water and not get diarrhea.Costs about $20. Use it before you try sterilizing the water, it’s not only cleaner but it’s ph is changed too.
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