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Offlineshroomsynergy
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What are the effects of EDTA on spores???
    #6062101 - 09/14/06 06:43 PM (17 years, 8 months ago)

i got some new vacutainers that have edta in them, but i was wondering if it would definatly kill spores, or what?? 

is there any way to successfully clean it out of the tubes if i need to??

this has been driving me nutty for a week now. lolol :crazy2:


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InvisibleTien
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Re: What are the effects of EDTA on spores??? [Re: shroomsynergy]
    #6062418 - 09/14/06 07:58 PM (17 years, 8 months ago)

if you have spare spores, fill up one of the containers with spore solution..let sit for few days, knock up a jar.
this way you'll know for sure.

I personally do not think that EDTA will do anything to the spores..but I don't know how it would interact with mycellium.

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Invisiblefastfred
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Re: What are the effects of EDTA on spores??? [Re: Tien]
    #6063075 - 09/14/06 10:31 PM (17 years, 8 months ago)

I doubt that anyone has studied the issue or done experiments, but why would you think that EDTA would cause any problems for spores?


-FF

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Offlineblindchameleon
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Re: What are the effects of EDTA on spores??? [Re: Tien]
    #6063420 - 09/15/06 12:50 AM (17 years, 8 months ago)

EDTA shouldn't hurt your spores. I know we use it as a buffer in lab, and I think I read somewhere that it's super abundant in consumer products.

Cosmetics, food preservatives, hydroponics, etc. I might have read that on wikipedia.

Wikipedia knows all.

How much EDTA we talking?

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Offlineshroomsynergy
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Re: What are the effects of EDTA on spores??? [Re: blindchameleon]
    #6074922 - 09/18/06 05:38 PM (17 years, 7 months ago)

i believe that EDTA is K3 (please correct me if i am mistaken) but i have heard people tell me that those vacutainers with EDTA are useless for mycology. i have recently been wondering if this was the casue of some bunk prints that i have made into syringes lately.

do spores react to high levels of K (potasium)?

there is about 1/4cc/ml per 10cc vacutainer...if that helps any.


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InvisibleTien
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Re: What are the effects of EDTA on spores??? [Re: shroomsynergy]
    #6085476 - 09/21/06 03:28 AM (17 years, 7 months ago)

I seriously can't see EDTA harming spore solution, it's been claimed that mushroom spores can survive a ride on an asteroid...so I doubt EDTA will have an adverse effect on them.

What I would like to know though, what is the interaction between EDTA and a liquid culture...I can't bring myself to beleive that thats a good mix.

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