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OfflinePartysan
newbie
Registered: 04/11/02
Posts: 32
Last seen: 21 years, 10 months
First signs of colonization
    #605021 - 04/11/02 04:46 AM (22 years, 2 months ago)

Hello All,

Hail to this FORUM!

i'm a total newbie in shroom cultivation, though do have some knowledge in cultivating other herbs :wink:

I have made 2 attempts to grow PC Golden Teacher with a PF rice-cake method.
After first attepmt of inoculating the sterilized substrate in a jar, i stored it in dark place and  have been observing it for a week. No signs of mycelium growth for 8 days! Not even a small white dot!

Then i made a second attempt with all necessary precautions (i.e. jars are cool enough, water in the syringe is cool, etc.). The jars were enoculated with a newly prepared spore syringe, though i used the same spore print (got only one from a Dutch shop). now again, it's been already 4 days, and i see no signs of mycelium.

I have undertaken a paranoid measures to sterilize everything around (i wiped everything with alcohol -  working area, syringe, lighter, scalpel, rubber gloves, etc.). So, i assume that contamination might still have a chance, but i see no signs of it so far, no signs of mycelium either..  :smile:)

Can it be a merely due to dead spores, or i just have to relax and wait for another 1-2 weeks? Note that spores were brought from Holland by air, they were packed in a bag, which was sitting for 3-4 hours in a cargo-trunk of aircraft. Perhaps, there was a temperature drop to well below 0'C? Questions, questions.....
Anybody ever experienced similar problems with the Golden Teacher strain?

Thanks a million for your replies..
Partysan 

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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: First signs of colonization [Re: Partysan]
    #605048 - 04/11/02 05:44 AM (22 years, 2 months ago)

What temps are you using to colonize the jars ?

4 days is a little early to see growth sometimes.

some haven't seen any growth in over 14 days time.

I usually see some growth in just a few days.

Try making a incubator...and setting it at 83 to 84 degrees. If you haven't already.

Good luck!



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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OfflineMrJingles
journeyman
Registered: 03/13/02
Posts: 53
Last seen: 22 years, 1 month
Re: First signs of colonization [Re: Roadkill]
    #605078 - 04/11/02 06:25 AM (22 years, 2 months ago)

I've had spores seamingly 'ruined' by being in the frindge(or not being in the fridge..)
I have no idea wat causes it tho..
I had a print that I successfuly spawned two different projects with.. I tried to use the same print about 1 month later......... NOTHING!
think mother nature can be a bitch sometimes..
Peace


--------------------
Giggidy giggidy boom boom boom

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OfflinePartysan
newbie
Registered: 04/11/02
Posts: 32
Last seen: 21 years, 10 months
Re: First signs of colonization [Re: Roadkill]
    #605162 - 04/11/02 08:32 AM (22 years, 2 months ago)

The jar s are kept in a dark place at roughly 75' F - room temperature. I monitor the temp with a thermometre..


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OfflinePartysan
newbie
Registered: 04/11/02
Posts: 32
Last seen: 21 years, 10 months
Re: First signs of colonization [Re: MrJingles]
    #605163 - 04/11/02 08:35 AM (22 years, 2 months ago)

the print was in a ziplock baggie, inside a Grower's kit, which was delivered from Amsterdam. The kit has been stored at room temperature.

Is it better to keep the spores in a fridge?

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OfflineAnnoA
Experimenter
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Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 month, 1 day
Re: First signs of colonization [Re: Partysan]
    #605175 - 04/11/02 08:51 AM (22 years, 2 months ago)

>The jar s are kept in a dark place at roughly 75' F

They should be kept at 80-85?F.

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: First signs of colonization [Re: Anno]
    #605216 - 04/11/02 09:46 AM (22 years, 2 months ago)

Well Anno....now we know why he isn't seeing any growth.



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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InvisibleUv1
enthusiast

Registered: 03/14/01
Posts: 149
Loc: Bloom County
Post deleted by users_request [Re: Partysan]
    #605340 - 04/11/02 11:37 AM (22 years, 2 months ago)


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OfflineLSD_4me
addict
Registered: 02/09/02
Posts: 416
Last seen: 22 years, 1 month
Re: First signs of colonization [Re: Uv1]
    #605383 - 04/11/02 12:31 PM (22 years, 2 months ago)

yea but they will still rehydrate in the jars if he didnt wait so this wouldnt be a cause for too much worry..

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OfflinePartysan
newbie
Registered: 04/11/02
Posts: 32
Last seen: 21 years, 10 months
Re: First signs of colonization [Re: Roadkill]
    #606058 - 04/12/02 02:56 AM (22 years, 2 months ago)

Aloha 2 all farmers!

Hey-hey-hey, i checked the jars today morning and guess what, the micelium is there... Hehehe....  5 out of 7 jars have bright white spots.

Interesting note - that old jar, which didn;t succeed last time, still shows no signs.
:frown:  Last time  I filled it the verm+brown rice powder, which was included in a Grower's kit from Holland. I re-sterilized it and tired this time, but still nothing.. This is a tall 250 ml glass, previously filled with French mustard. Of course i washed it thoroughly, but maybe the mustard smell, which is hard to kill, is a problem for fungus?  :wink: Or i assume that Dutch verm-rice is not good.

Note 2: one of the jars had about twice the amount of water in substrate mix, than recommended by PF tek. This jar has the most robust looking micelium. 

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