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Offlineinfinite420
truth seeker
Registered: 04/25/04
Posts: 53
Loc: Jersey Shore
Last seen: 14 years, 2 months
Sterile LC Transfers
    #6044392 - 09/09/06 04:03 PM (15 years, 1 month ago)

I want to prepare some LC jars but I am unsure of the method I want to use to transfer my LC to my substrate down the road. The syringe I have is only 1 1/2 inches long, therefore it won't even reach the bottom of a 1/2 pint jar. I was wondering if anyone had derived any alternative methods for transfering LC into a grain jar without using a syring, or a way to extract or pour LC into something smaller which i could then extract with a syringe. Very worse case scenario I know I can buy a longer needle but right now im looking for an alternative solution so I can use full pint or even quart jars to make my LC.

thanks for the help


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"all you toucn and all you see, is all your life will ever be" - you know who -


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Offlineseg_x
was here,chillin
Male User Gallery

Registered: 08/31/06
Posts: 368
Loc: Some place south.
Last seen: 6 years, 2 months
Re: Sterile LC Transfers [Re: infinite420]
    #6044425 - 09/09/06 04:16 PM (15 years, 1 month ago)

where did you get your syringe from?

don't add the .com or .org for respect of shroomery..
where you got the syringe is possible not a sponsor.
so with that in mind don't link, just us abbreviations.

and to answer your question, you could get a longer needle.

Or use a glove box to take the lid off (quickly),
then put your needle where you want it.
(try to do this quick as u can, don't forget to flame your needle)


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My Fall Mushrooms


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Offlineinfinite420
truth seeker
Registered: 04/25/04
Posts: 53
Loc: Jersey Shore
Last seen: 14 years, 2 months
Re: Sterile LC Transfers [Re: seg_x]
    #6044435 - 09/09/06 04:19 PM (15 years, 1 month ago)

ralph hooked me up

props for extremely fast shipping


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"all you toucn and all you see, is all your life will ever be" - you know who -


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OfflineSolidcell
tolerance++;
Male

Registered: 01/20/06
Posts: 754
Loc: Memphis & 53rd
Last seen: 13 years, 1 month
Re: Sterile LC Transfers [Re: infinite420]
    #6044478 - 09/09/06 04:39 PM (15 years, 1 month ago)

The needle will usually not reach the bottom unless you have a very long needle or really short jar. You should make the second hole on the lid for air intake far enough towards the edge and have the liquid level low enough that you can just tilt the jar on it's side to have the liquid come to the needle and yet not touch the polyfil in the other tube.

It also helps to have a stir plate to mix the solution first thus bringing the myc to all parts of the liquid, even the very top.

BTW, when you say your syringe is 1 1/2 inches, make sure to say needle instead. It didn't trip me up, but little things like that make it hard for newbs to understand what's going on when the vocabulary isn't consistent.


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Psychedelic Jar Project

"It is dangerous to be right when the government is wrong." -Voltaire

"Everything that I accepted as being true up to now I acquired from the senses or through the senses. However, I have occasionally found that they deceive me, and it is prudent never to trust those who have deceived us, even if only once." -Descartes


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Offlineindicaz
Lysol God
Male User Gallery

Registered: 04/13/02
Posts: 580
Loc: My Chair
Last seen: 12 years, 6 months
Re: Sterile LC Transfers [Re: Solidcell]
    #6045008 - 09/09/06 11:09 PM (15 years, 1 month ago)

can anyone suggest where to get a needle of the correct gauge 12g or 10g and atleast 5 or 6 inches long... Im currently using quart jars with 500ML of karo/water in them... and im faced with a hard time finding long aspiration needles or even hypoderic needles that are disposable and easily fit unto disposable syringes..

if anyone can spare one or knows where i can get it let me know..

peace
indi


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InvisiblePornoRob
He's not such abad guy
Male
Registered: 07/04/05
Posts: 175
Re: Sterile LC Transfers [Re: indicaz]
    #6045175 - 09/10/06 12:01 AM (15 years, 1 month ago)

The only needles I know of that are even close to that range are the stainless ones that you use with the auto repeater syringes. They are usually used for live stock and even those only go up to 3".


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Offlinesoulsizzle
nobody f**kswith The Jesus

Registered: 05/18/05
Posts: 632
Last seen: 10 years, 1 month
Re: Sterile LC Transfers [Re: PornoRob]
    #6045696 - 09/10/06 03:06 AM (15 years, 1 month ago)

When i create jars for liquid cultures, I create two holes in the lids. I then seal both holes with RTV silicon (found in the automotive section in just about any superstore). The silicon serves two purposes. First, it creates a self-healing injection port to reduce contamination. Second, even with the needle inserted through the silicon, you can tip the jars sideways or upside down without any leaking.


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OfflineMycomyth
Demented Avenger
Male User Gallery

Registered: 03/19/06
Posts: 341
Loc: At the crux of the matter...
Last seen: 5 years, 7 months
Re: Sterile LC Transfers [Re: soulsizzle]
    #6046233 - 09/10/06 11:32 AM (15 years, 1 month ago)

Type "inksupply" without the quotes into the address bar of your browser and hit ctrl + enter. Scroll halfway down that page and you'll find 4", 16ga, leur lock ink cartridge refill needles.

FWIW,
M


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Wave upon wave of demented avengers marched cheerfully out of obscurity into the dream.


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Offlinealpiner
goober

Registered: 08/27/03
Posts: 226
Loc: USA
Last seen: 12 years, 5 months
Re: Sterile LC Transfers [Re: Mycomyth]
    #6046573 - 09/10/06 02:37 PM (15 years, 1 month ago)

16 gauge is all you need, nothing bigger is needed
a inch and a half is enough, you just need to have a hole close to the rim of the jar, you could use gallon jars if you want to, it doesn't matter you just tip the jar so you can get LC to your needle


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