Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Boomr Bag   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinelurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
Extra uncolonized substrate, can I add it to my casing trays?
    #6038353 - 09/07/06 12:47 PM (17 years, 5 months ago)

My friend has a bunch of extra substrate (rye) that never colonized (the spores were weak maybe).

Could one theoretically mix in the (sterilized of course) extra uncolonized substrate with the colonized substrate in the casing trays to add bulk (I was thinking a 1:1 ratio of colonized to uncolonized)?

If so, should I add both (the uncolonized and colonized) to the trays and case immediately and incubate a few days as regular or should I leave uncased (and covered with foil of course) and incubate a couple days before casing and incubating a couple more days before fruiting?

Does introducing uncolonized (but sterilized) substrate to the casing procedure open up the window of contamination any wider? If so, would one suggest two fruiting chambers (this would be easy to do): one with purely colonized substrate and one with the uncolonized/colonized mix?

My friend mainly wants to use the uncolonized substrate and not have to throw it away or go through the inoculation process again, which he considers wasteful (of time or resources).

Peace,
Lurking


Extras: Filter Print Post Top
Offlinelurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
    #6038362 - 09/07/06 12:51 PM (17 years, 5 months ago)

Here's another option that was thought of. Tell me which is better, if you care to.

My friend creates a glove box and uses mycelium from a colonized jar to innoc the uncolonized jars. This would require opening the colonized jars and the uncolonized jars (or he remembers something about squirting some sterile water into colonized jars, shaking, then sucking the water back up and inoc'ing into uncolonized jars).

This would cause some delay but would possibly be less traumatic to the casings and less chance for contam?

Peace


Extras: Filter Print Post Top
InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
    #6038395 - 09/07/06 01:05 PM (17 years, 5 months ago)

2 words...

contam city!~


you don't want to add uncolonized (but sterilized) substrate to anything...
especially if the substrate is some kind of grains.

the exception of the rule...a bulk substrate...like horse poo, cow manure, worm castings, compost...etc.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Extras: Filter Print Post Top
Offlinelurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: Roadkill]
    #6038485 - 09/07/06 01:29 PM (17 years, 5 months ago)

hmm, OK. What about grain to grain as a method of "colonizing the uncolonized" (that sounds like the title of a strange short story)?

Peace :tongue2:


Extras: Filter Print Post Top
Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
    #6038502 - 09/07/06 01:33 PM (17 years, 5 months ago)

if you inoculated jars of grain and they didn't colonize...

throw them out.

empty the jars, clean them, and fill them with NEW grain.

once the NEW jars are complete and cool,

do a grain to grain transfer into them from a master colonized jar.


--------------------



Extras: Filter Print Post Top
Offlinelurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: monstermitch]
    #6038537 - 09/07/06 01:46 PM (17 years, 5 months ago)

Mitch, can you give me a reason for chucking the jars? I am hesitant to waste in that fashion without understanding why. The micro-climate inside the jars appears to me to be exactly the same (humidity wise). Is there a reason why they could be "bad" that is not visible to the human eye?

Thanks


Extras: Filter Print Post Top
OfflineMonChe
RevolutionaryMonkey
Male User Gallery

Registered: 08/16/06
Posts: 100
Loc: Canada
Last seen: 9 years, 10 months
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
    #6038630 - 09/07/06 02:26 PM (17 years, 5 months ago)

bacteria could be one really good reason to chuck them. you can't see, but could smell it. But then there is Some reason why the myc was stalled/killed off in those jars. It's just FAR safer to start fresh.


--------------------
MonChe, Leading the monkeys against the tyranny of the apes in the Gorilla wars!


Extras: Filter Print Post Top
Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
    #6038742 - 09/07/06 03:17 PM (17 years, 5 months ago)

Quote:

lurking said:
Mitch, can you give me a reason for chucking the jars? I am hesitant to waste in that fashion without understanding why. The micro-climate inside the jars appears to me to be exactly the same (humidity wise). Is there a reason why they could be "bad" that is not visible to the human eye?

Thanks




as stated, bacteria could be present.

moreover, the water content now is not the same.

don't get me wrong, it can be done.
I've doine it before.

when I did it, I shot 30cc of LC into each quart jar.
yup, you heard right.

they were very dry, and had been sitting out for about a month.


there could be contaminate spores in your jars or bacteria,
and it's just waiting for moisture to bloom.

your filter setup is not perfect I would wager.
a spore or two probably made it in there over the last however long it's been sitting around.
adding inoculant could allow that spore to spread or that bacteria to grow.


grain/spawn is cheap, and it only takes a day or two start to finish
to make it.

better safe than anything else.


--------------------



Extras: Filter Print Post Top
Offlinelurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: monstermitch]
    #6039008 - 09/07/06 04:47 PM (17 years, 5 months ago)

True, better safe... Thanks for the more thorough explanation.

Peace,
Lurking


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Boomr Bag   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Re: Mixing substrate with casing! The next big thing?
( 1 2 all )
LinkA 6,251 21 01/15/00 03:52 PM
by Anonymous
* Poo Substrate or Casing???? indicaz 985 4 05/20/02 03:45 PM
by bassplayer74
* Best bulk substrate and casing? mycologist 11,605 8 09/09/01 04:47 PM
by Shdwstr
* Quick Reference Substrates and Casings? maria420 648 2 07/09/01 05:29 AM
by Anno
* Earthworm Castings for bulk substrate and casing? BlueBoy 1,938 2 03/14/02 01:32 AM
by BlueBoy
* Substrate and Casing mixture blitz 1,234 4 06/15/02 05:28 AM
by blitz
* Re: Best Substrate for Casing? BoNeThuG 790 3 04/25/01 01:36 PM
by BrownPastures
* Re: Different substrates, same casing? Anonymous 462 2 07/29/00 12:41 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,711 topic views. 14 members, 112 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.046 seconds spending 0.007 seconds on 12 queries.