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lurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
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Extra uncolonized substrate, can I add it to my casing trays?
#6038353 - 09/07/06 12:47 PM (17 years, 5 months ago) |
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My friend has a bunch of extra substrate (rye) that never colonized (the spores were weak maybe).
Could one theoretically mix in the (sterilized of course) extra uncolonized substrate with the colonized substrate in the casing trays to add bulk (I was thinking a 1:1 ratio of colonized to uncolonized)?
If so, should I add both (the uncolonized and colonized) to the trays and case immediately and incubate a few days as regular or should I leave uncased (and covered with foil of course) and incubate a couple days before casing and incubating a couple more days before fruiting?
Does introducing uncolonized (but sterilized) substrate to the casing procedure open up the window of contamination any wider? If so, would one suggest two fruiting chambers (this would be easy to do): one with purely colonized substrate and one with the uncolonized/colonized mix?
My friend mainly wants to use the uncolonized substrate and not have to throw it away or go through the inoculation process again, which he considers wasteful (of time or resources).
Peace, Lurking
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lurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
#6038362 - 09/07/06 12:51 PM (17 years, 5 months ago) |
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Here's another option that was thought of. Tell me which is better, if you care to.
My friend creates a glove box and uses mycelium from a colonized jar to innoc the uncolonized jars. This would require opening the colonized jars and the uncolonized jars (or he remembers something about squirting some sterile water into colonized jars, shaking, then sucking the water back up and inoc'ing into uncolonized jars).
This would cause some delay but would possibly be less traumatic to the casings and less chance for contam?
Peace
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
#6038395 - 09/07/06 01:05 PM (17 years, 5 months ago) |
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2 words...
contam city!~
you don't want to add uncolonized (but sterilized) substrate to anything... especially if the substrate is some kind of grains.
the exception of the rule...a bulk substrate...like horse poo, cow manure, worm castings, compost...etc.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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lurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: Roadkill]
#6038485 - 09/07/06 01:29 PM (17 years, 5 months ago) |
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hmm, OK. What about grain to grain as a method of "colonizing the uncolonized" (that sounds like the title of a strange short story)?
Peace
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
#6038502 - 09/07/06 01:33 PM (17 years, 5 months ago) |
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if you inoculated jars of grain and they didn't colonize...
throw them out.
empty the jars, clean them, and fill them with NEW grain.
once the NEW jars are complete and cool,
do a grain to grain transfer into them from a master colonized jar.
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lurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: monstermitch]
#6038537 - 09/07/06 01:46 PM (17 years, 5 months ago) |
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Mitch, can you give me a reason for chucking the jars? I am hesitant to waste in that fashion without understanding why. The micro-climate inside the jars appears to me to be exactly the same (humidity wise). Is there a reason why they could be "bad" that is not visible to the human eye?
Thanks
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MonChe
RevolutionaryMonkey


Registered: 08/16/06
Posts: 100
Loc: Canada
Last seen: 9 years, 10 months
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
#6038630 - 09/07/06 02:26 PM (17 years, 5 months ago) |
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bacteria could be one really good reason to chuck them. you can't see, but could smell it. But then there is Some reason why the myc was stalled/killed off in those jars. It's just FAR safer to start fresh.
-------------------- MonChe, Leading the monkeys against the tyranny of the apes in the Gorilla wars!
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: lurking]
#6038742 - 09/07/06 03:17 PM (17 years, 5 months ago) |
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Quote:
lurking said: Mitch, can you give me a reason for chucking the jars? I am hesitant to waste in that fashion without understanding why. The micro-climate inside the jars appears to me to be exactly the same (humidity wise). Is there a reason why they could be "bad" that is not visible to the human eye?
Thanks
as stated, bacteria could be present.
moreover, the water content now is not the same.
don't get me wrong, it can be done. I've doine it before.
when I did it, I shot 30cc of LC into each quart jar. yup, you heard right.
they were very dry, and had been sitting out for about a month.
there could be contaminate spores in your jars or bacteria, and it's just waiting for moisture to bloom.
your filter setup is not perfect I would wager. a spore or two probably made it in there over the last however long it's been sitting around. adding inoculant could allow that spore to spread or that bacteria to grow.
grain/spawn is cheap, and it only takes a day or two start to finish to make it.
better safe than anything else.
--------------------
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lurking
Stranger
Registered: 08/27/06
Posts: 14
Last seen: 17 years, 2 months
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Re: Extra uncolonized substrate, can I add it to my casing trays? [Re: monstermitch]
#6039008 - 09/07/06 04:47 PM (17 years, 5 months ago) |
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True, better safe... Thanks for the more thorough explanation.
Peace, Lurking
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