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ty1er4499
Stranger
Registered: 07/20/06
Posts: 14
Last seen: 14 years, 1 month
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Stalled colonization.
#6009902 - 08/29/06 08:59 PM (17 years, 6 months ago) |
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Out of 6 jars 4 began colonization and 2 contamed. 1 looks really good, while the other 3 started out pretty good, but slowed or stopped colonization. The stalled ones have mycellium circles around the injection points...but stopped spreading. My temps are good. I think maybe i used too little or too much water. What do you guys think is the likely cause? and what is the ideal ratio of vermiculite/water/brf? Im using fine vermiculite if that makes a diference.
-------------------- Play Plasma Pong!
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Stalled colonization. [Re: ty1er4499]
#6010904 - 08/30/06 06:32 AM (17 years, 6 months ago) |
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any tape on jar lid holes ? remove if so... the ratio is 2:1:1 verm:brf:water
-------------------- Admin @ mycotopia.net Mycotopia
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creamcorn
mad scientist
Registered: 03/13/06
Posts: 2,962
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Re: Stalled colonization. [Re: Hippie3]
#6010949 - 08/30/06 07:46 AM (17 years, 6 months ago) |
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any odors to the jars?
bacterial contamination is a common yet often overlooked cause for stalling... it stands to reason if 2 contamed you might have other contams... if there's a scent to your jars that wasn't there when you started, they're toast.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Stalled colonization. [Re: ty1er4499]
#6011012 - 08/30/06 08:35 AM (17 years, 6 months ago) |
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Quote:
Im using fine vermiculite if that makes a diference.
your substrate might be too wet...
since you are using fine verm.
this can cause stalling and contams in the jars.
also listen to the advice given by the other 2 growers above me.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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ty1er4499
Stranger
Registered: 07/20/06
Posts: 14
Last seen: 14 years, 1 month
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Re: Stalled colonization. [Re: Roadkill]
#6011226 - 08/30/06 10:30 AM (17 years, 6 months ago) |
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No tape over holes...just loose foil lids. I think I probably overdid the water because I added a little extra water to the mix. I figured mycelium can grow in 100% water so id better have it on the wetter end of the spectrum. Is the amount of water a very sensitive factor? Oh well...I'm learning alot from my mistakes. I don't think those jars have any bacterial contams, is there anything i can do to get them going?
On the upside my LC was a success, so ill probably be making some new jars in the next couple days. Hopefully this time ill get it right.
-------------------- Play Plasma Pong!
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creamcorn
mad scientist
Registered: 03/13/06
Posts: 2,962
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Re: Stalled colonization. [Re: ty1er4499]
#6011258 - 08/30/06 10:38 AM (17 years, 6 months ago) |
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mycelium growing in 100% water is a totally different story
mycelium digests food by excreting enzymes to break down the food into materials they can then absorb
if your substrate is too wet, those excreted enzymes are diluted and rendered less effective or ineffective
in an LC, they're surrounded at every angle by ready to absorb nutrition - that's why simple sugars are used to prepare LCs, no "digestion" is necessary.
brown rice isn't a simple sugar though and needs to be broken down into it.
if too wet is really the problem, they will likely eventually go. it will just take longer... perhaps much longer.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Stalled colonization. [Re: creamcorn]
#6011305 - 08/30/06 10:55 AM (17 years, 6 months ago) |
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Quote:
creamcorn said:
if your substrate is too wet, those excreted enzymes are diluted and rendered less effective or ineffective
if too wet is really the problem, they will likely eventually go. it will just take longer... perhaps much longer.
or they might never colonize!~
I've accidently made some really wet pf jars(brf/verm)...
and it took forever to colonize.
I'd guess that this is one of the main reasons that some people's jars take so long to fully colonize!~ The ones that take 3 to 4 weeks or longer...that is!~
compared to 7 to 14 days.
If you are using fine verm...use less water in your ratio. Just add enough water to get your Verm wet...without a pool of water in the mixing bowl. Then add the brf to the damp verm.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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