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 ChrisL_NL
Stranger
Registered: 02/09/02
Posts: 19
Loc: The Hague, NL
Last seen: 21 years, 6 months
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 Using fresh mushrooms to start a cake? 
#600740 - 04/06/02 05:46 PM (21 years, 7 months ago) |
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Can this be done? If I have, say 15 grams of fresh shrooms, could I just cut them up and place them in the substrate?
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"We can't stop here, this is bat country!"
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Canisdormit
journeyman
Registered: 11/10/01
Posts: 84
Last seen: 20 years, 1 month
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 Re: Using fresh mushrooms to start a cake? [Re: ChrisL_NL]
#600751 - 04/06/02 06:06 PM (21 years, 7 months ago) |
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I think you'd have the best success at that if the tissue you use comes from the very bottom of the stem, but it still might not work.
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If humans are what they eat, I'm just a huuuuuuge mushroom.
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Alpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 3 days
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Re: Using fresh mushrooms to start a cake? [Re: ChrisL_NL]
#600759 - 04/06/02 06:17 PM (21 years, 7 months ago) |
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Take one of your bigger shrooms...
cut it up, with a food procesor, or by hand (food processor recomended)
mix with sterile water...
inoculate with this slurry of goodness.
note: ive heard your shouldn't use the cap if your doing this.
hope that helps.
alpherat
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blasted holes into night until she bled sunshine
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 DreaMaTrix
Shaman I am
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
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Re: Using fresh mushrooms to start a cake? [Re: Alpheratus]
#600778 - 04/06/02 06:31 PM (21 years, 7 months ago) |
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>Take one of your bigger shrooms...
cut it up, with a food procesor, or by hand (food processor recomended)
mix with sterile water...
inoculate with this slurry of goodness
Add some hydrogen peroxide too,after shredding, if youre worried about contams. Peroxide can be used with mycelium, which is what shrooms are, but kills spores.
Good luck
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"We are the one's we have been waiting for" - Hopi saying
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Zen Peddler
 
Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: Using fresh mushrooms to start a cake? [Re: ChrisL_NL]
#600794 - 04/06/02 06:47 PM (21 years, 7 months ago) |
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Usually cloning - which is what you are doing - works best with a bit of h202 - dilute it a bit with water if your worried.
cut the mushrooms in half and take a piece of tissue from the inside of the area where the cap meets the stem - dip this in h202 and then grind it through a clean coffee grinder - this will work wonders!
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clearblue
newbie
Registered: 03/04/02
Posts: 27
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Re: Using fresh mushrooms to start a cake? *DELETED* [Re: Zen Peddler]
#601286 - 04/07/02 11:12 AM (21 years, 7 months ago) |
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Post deleted by clearblue
Edited by clearblue (04/07/02 11:17 AM)
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LSD_4me
addict
Registered: 02/09/02
Posts: 416
Last seen: 21 years, 7 months
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Re: Using fresh mushrooms to start a cake? [Re: clearblue]
#601369 - 04/07/02 02:10 PM (21 years, 7 months ago) |
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you wouldnt want to use a mortar and pestle at all, that wouldnt be sterile.... id say leave the food processor on longer if the pieces arent small enough, a coffee grinder has been used neither i nor afoaf has done this so i cant give you ant more help than that... i just know a mortar and pestle would give contams a good chance of invading, also it wouldnt be good to smash the fresh shroom, if you some way had a Dry shroom that was dried in a totally sterile environment and then had a totally sterile pestle and mortar and a glove box then that MIGHT work but its still kinda unlikely
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DreaMaTrix
Shaman I am
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
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Re: Using fresh mushrooms to start a cake? [Re: LSD_4me]
#601378 - 04/07/02 02:26 PM (21 years, 7 months ago) |
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>if you some way had a Dry shroom that was dried in a totally sterile environment and then had a totally sterile pestle and mortar and a glove box then that MIGHT work but its still kinda unlikely
A dried shroom wouldn't work for innoculation.
Blending will create *some* pieces small enough to be sucked into a syringe
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"We are the one's we have been waiting for" - Hopi saying
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