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Invisiblealtarego
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Cloning Mex Sclerotia? ( -> Bio, Workman, et al.)
    #597738 - 04/03/02 04:41 PM (15 years, 3 months ago)

OK -

I have several qt jars of "B" strain sclerotia. Before I harvest, I was planning to clone several of them onto agar.

Has anyone done this? I haven't seen any postings about it yet, so I thought I'd ask.

Thanks for the info, if anyone has any, and I'll be sure to post results as they arise.

********Sorry, that should read "A" strain, not "B".******

- AE



Edited by altarego (04/04/02 04:31 PM)


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OfflineAnnoA
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Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: altarego]
    #597792 - 04/03/02 06:08 PM (15 years, 3 months ago)

Yes, it works just like cloning normal mushroom tissue.


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Anonymous

Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: altarego]
    #598035 - 04/03/02 11:42 PM (15 years, 3 months ago)

Invitro B strain? Congrats!!!


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Invisiblefeito
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Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: ]
    #598095 - 04/04/02 12:59 AM (15 years, 3 months ago)

How do I get flesh w/o getting the "goo"?!
If you have grown em, ya know what I mean.
And are chances better they will produce BETTER next round?(sclerotia wise).


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OfflineWorkmanV
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Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: feito]
    #598415 - 04/04/02 10:51 AM (15 years, 3 months ago)

I think you are beating a dead horse trying to get invitro sclerotia from the B strain. What you have is about all you will get despite your best efforts. Try casing your substrate and you will get a decent amount.

The "goo" is just metabolites trapped between the sclerotia and the glass. Try dropping the sclerotia in a some H2O2 then either cutting into it for some cloning tissue or just drop the whole thing in another jar. Small seedlike sclerotia found deep within the substrate are ideal for dropping into new jars after washing with H2O2.


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