Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Mix

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Cloning Mex Sclerotia? ( -> Bio, Workman, et al.)
    #597738 - 04/03/02 02:41 PM (21 years, 10 months ago)

OK -

I have several qt jars of "B" strain sclerotia. Before I harvest, I was planning to clone several of them onto agar.

Has anyone done this? I haven't seen any postings about it yet, so I thought I'd ask.

Thanks for the info, if anyone has any, and I'll be sure to post results as they arise.

********Sorry, that should read "A" strain, not "B".******

- AE



Edited by altarego (04/04/02 02:31 PM)


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 hours
Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: altarego]
    #597792 - 04/03/02 04:08 PM (21 years, 10 months ago)

Yes, it works just like cloning normal mushroom tissue.


Extras: Filter Print Post Top
Anonymous

Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: altarego]
    #598035 - 04/03/02 09:42 PM (21 years, 10 months ago)

Invitro B strain? Congrats!!!


Extras: Filter Print Post Top
Invisiblefeito
hillbillyhippykiller

Registered: 03/06/02
Posts: 75
Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: ]
    #598095 - 04/03/02 10:59 PM (21 years, 10 months ago)

How do I get flesh w/o getting the "goo"?!
If you have grown em, ya know what I mean.
And are chances better they will produce BETTER next round?(sclerotia wise).


Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 1 hour, 58 minutes
Trusted Cultivator
Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: feito]
    #598415 - 04/04/02 08:51 AM (21 years, 10 months ago)

I think you are beating a dead horse trying to get invitro sclerotia from the B strain. What you have is about all you will get despite your best efforts. Try casing your substrate and you will get a decent amount.

The "goo" is just metabolites trapped between the sclerotia and the glass. Try dropping the sclerotia in a some H2O2 then either cutting into it for some cloning tissue or just drop the whole thing in another jar. Small seedlike sclerotia found deep within the substrate are ideal for dropping into new jars after washing with H2O2.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds High THC Strains   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Mix


Similar ThreadsPosterViewsRepliesLast post
* Mexicana Strain A Scelortia Cloning Question. bent 3,588 9 06/08/04 02:19 AM
by RogerRabbit
* Any-one done this before ? Atlantis Cloning ;) MeneerCactus 1,921 10 04/06/03 12:17 AM
by MeneerCactus
* Re: Can this be Sclerotia on my PF casings? psylohassee 1,213 5 04/17/01 10:17 AM
by CLuB99
* Dry tissue cloning/spore extraction ??? Bi0TeK 2,474 2 06/25/03 10:45 AM
by Bi0TeK
* growing mex A-strain on rice B.I.O. 2,975 8 03/07/02 08:30 AM
by Workman
* Bacterie on sclerotia that is sold? porsches 1,168 5 11/24/02 01:40 PM
by porsches
* Mexicana Sclerotia Invitro
( 1 2 all )
WorkmanV 10,878 26 08/24/05 05:30 PM
by Juan_del_Pueblo
* Mex B strain Mr Wobblehead 818 1 01/07/03 02:48 AM
by Workman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
1,830 topic views. 0 members, 13 guests and 6 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 12 queries.