Home | Community | Message Board


Sporeworks
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Cloning Mex Sclerotia? ( -> Bio, Workman, et al.)
    #597738 - 04/03/02 04:41 PM (16 years, 1 month ago)

OK -

I have several qt jars of "B" strain sclerotia. Before I harvest, I was planning to clone several of them onto agar.

Has anyone done this? I haven't seen any postings about it yet, so I thought I'd ask.

Thanks for the info, if anyone has any, and I'll be sure to post results as they arise.

********Sorry, that should read "A" strain, not "B".******

- AE



Edited by altarego (04/04/02 04:31 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 8 days
Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: altarego]
    #597792 - 04/03/02 06:08 PM (16 years, 1 month ago)

Yes, it works just like cloning normal mushroom tissue.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: altarego]
    #598035 - 04/03/02 11:42 PM (16 years, 1 month ago)

Invitro B strain? Congrats!!!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefeito
hillbillyhippykiller

Registered: 03/06/02
Posts: 75
Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: ]
    #598095 - 04/04/02 12:59 AM (16 years, 1 month ago)

How do I get flesh w/o getting the "goo"?!
If you have grown em, ya know what I mean.
And are chances better they will produce BETTER next round?(sclerotia wise).


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,535
Loc: Washington, USA
Last seen: 14 minutes, 11 seconds
Trusted Cultivator
Re: Cloning Mex Sclerotia? ( -> Bio, Workman, et al.) [Re: feito]
    #598415 - 04/04/02 10:51 AM (16 years, 1 month ago)

I think you are beating a dead horse trying to get invitro sclerotia from the B strain. What you have is about all you will get despite your best efforts. Try casing your substrate and you will get a decent amount.

The "goo" is just metabolites trapped between the sclerotia and the glass. Try dropping the sclerotia in a some H2O2 then either cutting into it for some cloning tissue or just drop the whole thing in another jar. Small seedlike sclerotia found deep within the substrate are ideal for dropping into new jars after washing with H2O2.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Mexicana Strain A Scelortia Cloning Question. bent 2,916 9 06/08/04 04:19 AM
by RogerRabbit
* Any-one done this before ? Atlantis Cloning ;) MeneerCactus 1,483 10 04/06/03 03:17 AM
by MeneerCactus
* Re: Can this be Sclerotia on my PF casings? psylohassee 915 5 04/17/01 12:17 PM
by CLuB99
* Dry tissue cloning/spore extraction ??? Bi0TeK 1,675 2 06/25/03 12:45 PM
by Bi0TeK
* growing mex A-strain on rice B.I.O. 1,833 8 03/07/02 10:30 AM
by Workman
* Bacterie on sclerotia that is sold? porsches 1,013 5 11/24/02 03:40 PM
by porsches
* Mexicana Sclerotia Invitro
( 1 2 all )
WorkmanV 8,585 26 08/24/05 07:30 PM
by Juan_del_Pueblo
* Mex B strain Mr Wobblehead 714 1 01/07/03 04:48 AM
by Workman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
1,432 topic views. 1 members, 4 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Original Seeds Store - Cannabis Seeds
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.005 seconds on 19 queries.