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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Grow Log - First Try (UPDATES w/ PICS - 9/4/06)
    #5976036 - 08/18/06 02:28 PM (17 years, 5 months ago)

A friend has started growing at his house, but I am the scientific mind doing the work.

OK - Pics will come soon (logs are no fun without pics).

3 Strains of Psilocybe Cubensis:
    *Blue Meanie
    *PES Hawaiian
    *Pink Buffalo
Faze One - Print to Syringe

Supplies:
    * 4 jars w/lids
    * 3 shot glasses
    * Distilled water
    * 9 sterile 10cc syringes
    * 3 sterile 3cc syringes
    * 70% Alcohol
    * Cotton balls
    * Lysol/Oust
    * 50/50 bleach water
    * Paper towels
    * Face Mask
    * Glove Box
    1) I washed my hands with antibacterial soap, follow with a rub down of pure bleach (I did this often.  I also cleaned all surfaces with the same soap, followed by 50/50 bleach water, and a good spray of Lysol.
    2) I took 3 jars, dropped a shot glass into each one and filled with water.
    3) I PCed those jars for 70 minutes at 15psi and let cool in the PC over night.
    4) I prepared my glove box by spraying it down with Lysol and placing wet paper towels, dipped in the bleach water, and lined the bottom of the box.
    5) with clean hand and a good spray of oust into the air, I opened the PC, places the 3 jars inside, along with another jar filled with cotton balls and 70% alcohol.
    6) I sprayed the box again with Lysol, closed the box and let it sit for 10 minutes.
    7) I opened the first jar and then opened 3 10cc and 1 3cc sterile syringes (one at a time) and filled them with the water in the jar and recapped them.
    8 ) The shot glasses were still covered in water; carefully I slid the shot glass out, making sure not to touch the top edge or inside.
    9) Then I opened the first spore print (Blue Meanie) - I carefully held the foil by the corner edge, took a syringe and took off the top, used the needle to scrape of 1/4 of the print into the shot glass, and expelled a little syringe water onto the foil to wash the spores into the glass.
    10) I took the syringe and pushed out and pulled in several times to break up the spores, then after pulling them all in, I wiped the needle with an alcohol cotton ball and placed the cap back on.
    11) I repeated with the remaining syringes and I made sure the last syringe for each print was the 3 cc.
    12) I did this again, at the same time, with the PES Hawaiian too - I waited several days and did the Pink Buffalo syringes separately (I had learned allot doing the first 2 prints, I though I should not risk doing all three at the same time due to contaminates and lack of experience - it was a good idea because when I did the last print, I felt like a pro).
Faze Two - Syringe to Inoculation

Supplies:
    * 30 wide mouth 1/2 pint jars
    * 3 wide mouth 1 pint jars
    * 6 1 quart jars
    * Hammer/nail
    * Star Sticker
    * 5/8 drill bit
    * 7/8 vinyl tubing
    * Polyfill
    * Silicon
    * Vermiculite
    * Organic BRF
    * Whole organic millet
    * Whole organic quinoa
    * Spring and Distilled water
    * Foil
    * Tyvek
    * Rubber Bands
    * Microspore tape
    * PC
    * Glove Box
    * Cotton balls
    * Lysol/Oust
    * 50/50 bleach water
    * Paper towels
    * Face Mask
Jar Preparation
    1) I placed a star sticker in the middle of one of the ½ lids, used a ruler and marked 5 holes, evenly spaced – Using the hammer and nails; I punched 5 holes into all 30 lids
    2) Using the drill bit, I drilled a 5/8 hole in the remaining pint and quart lids, plus one nail hole.  I pulled the tubing through, trimmed the bottom/inside, and left 3 inches above/outside.  I sealed both the inside and outside with silicon and covered the nail hole with silicon from the inside.  I let it cure 48 hours.
    3) I stuffed polyfill into the top 1 ½ inch of the tubing.
Substrate Preparation

½ pint BRF jars:
    1) For the ½ pint jars, I used 2 recipes.  The first 10 jars were filled with ½ vermiculite, ¼ BRF, and ¼ spring water.  The other 20 were filled with 2/3 vermiculite, ¼ BRF, and ¼ spring water (first recipe looked too moist).
    2) I filled all the jars with the BRF mix, up to ¾ of an inch from the top and used a dry cotton ball to clean the inside edge. 
    3) I then filled the jars to the top with dry vermiculite, cover w/ lids, and a layer of foil secured by rubber bands.
    4) I PCed the jars for 70 minutes at 15psi, some cooled on the counter top over night, some cooled in the PC.
1 pint LC jars:
    1) I added 4 ccs of organic honey to 400ml of distilled water.  I ran this through coffee filters 3 times.
    2) I filled each of the 3 quart jars with 400lm of this honey water mix, plus 2 marbles and PCed for 30 minutes at 15 psi (too long – I had some sediment, but used it anyway).
1 quart jars:
    1) I soaked the millet in 2 jars for 24 hours – I also soaked the quinoa for 24 hours
    2) Then I simmered both grains for 20 to 40 minutes.  The quinoa looked perfect, filled but not exploded. The millet totally exploded, but I used it anyway – I let it drain for 10 minutes.
    3) I added the millet to 3 of the quart jars and the quinoa to the other 3, added the lids, and covered with foil secured by a rubber band.
    4) I PCed all the jars for 70 minutes at 15 psi, I let them cool in the PC.
Substrate results:
    * 10 of the 30 ½ pint jars look a little too moist so I switched to the 2/3 vermiculate recipe
    * The 1 pint LC jars did caramelize with a good amount of sediment – I used anyway
    * Because the millet exploded during simmering, the starches turned it into a ball of dough that would not break apart – they when in the trash (except the jars).
    * 1 of the quinoa jars was water logged – mainly because I help the jar in the fridge for 48 hours after simmering and before PCing – I used anyway
    * 2 of the quinoa jars looked perfect after PC (and still do)
Jar Inoculation

½ pint jars:
    1) I did these on different days during the week.
    2) I followed similar cleaning and disinfecting (counter tops, glove box, and myself) as I did for the print to syringe.
    3) I inoculated each jar with between 1 cc to 2.5 ccs each – my needles were only 1 inch, so it barley got to the substrate on the first 10 jars (all Blue Meanie).  The next sets were the remaining 20 jars with Hawaiian and Pink Buffalo, being more careful with the syringe being below the vermiculite layer and against the glass.  I upped the amount to 2.5 ccs each
    4) The first 10 jars were covered with microspore tape for 3 days and were then removed.
    5) The next 10 jars had “tape tents”
    6) The last 10 jars were covered with Tyvek
1 pint LC jars:
    1) I was clean, but not as much so as the ½ pint.
    2) I filled the air with oust.
    3) I wiped the inoculation hole with 70% alcohol
    4) Opened a 3cc syringe, wiped down with 70% alcohol
    5) Quickly inserted the needle and expelled all 3 ccs
    6) I repeated for the remaining 2 jars
1 quart jars:
    1) Similar to the LC jars
    2) I added 2.5 ccs each
Inoculation results:
    * ½ pint jars are inoculated with 3 strains, 10 jars per strain – oldest jars are 5-6 days old and show no contaminates and barely visible mycelium growth (99% sure it is mycelium)
    * The 1 pint LC jars look good so far, despite being caramelized
    * It’s too soon to know about the 1 quart jars, but I’m hopeful about the quinoa
Faze Three – Incubation
    * Using a TiT at 84, covered for one week at a time (glad it’s not in my house so I won’t peek)
More later  :cool:


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Edited by fromthemoon (09/03/06 01:38 PM)


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InvisibleHank, FTW
Looking for the Answer

Registered: 05/04/06
Posts: 3,912
Re: Grow Log - First Try [Re: fromthemoon]
    #5976054 - 08/18/06 02:33 PM (17 years, 5 months ago)

Sounds like you are doing this by the book. Good luck, and I hope I am as diligent for my first grow! :smile:


--------------------
Capliberty:

"I'll blow the hinges off your freakin doors with my trips, level 5 been there, I personally like x, bud, acid and shroom oj, altogether, do that combination, and you'll meet some morbid figures, lol
Hell yeah I push the limits and hell yeah thats fucking cool, dope, bad ass and all that, I'm not changing shit, I'm cutting to to the chase and giving u shroom experience report. Real trippers aren't afraid to go beyond there comfort zone "

:rofl:


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InvisibleSpookerShroom
Shroomerite
Registered: 06/12/06
Posts: 244
Re: Grow Log - First Try [Re: Hank, FTW]
    #5976742 - 08/18/06 06:47 PM (17 years, 5 months ago)

Quote:

alpharedecho said:
Sounds like you are doing this by the book. Good luck, and I hope I am as diligent for my first grow! :smile:




:thumbup:

Also, If your friend invested the same money and time into some rye/popcorn/WBS jars and some poo, he would get 10-100 times more out of his money.

But, starting with the PF is also a great way to get into this hobby!!!


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: SpookerShroom]
    #5976849 - 08/18/06 07:48 PM (17 years, 5 months ago)

I haven't checked the quinoa quart jars (and won't till Sunday), but they looked really good as far as moisture and airyness (is that a word). They are 3/4 full (a little more). I really liked the idea of trying sometime different while following the PF tek at the same time (plus the LC). Since quinoa is a grain of the Aztecs and the Aztecs had some of the greatest respect for the shroom. Plus the nuts!

I'm looking for h/poo (and found a near by horse ranch that I can try [fucking buses]) - but if that doesn't come through, I'll do the 40/60 coir/vermiculite casting with that.

9 (one of each species) of the PF jar will simply be dunked and rolled, 9 will be wrapped in Sphagnum Moss imported from New Zealand (I like working with exotic elements and get as organic as I can) for a step up play on the dunk and roll. 9 will be broken and casted (either the 40/60 or h/poo if I can get it)


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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InvisibleSpookerShroom
Shroomerite
Registered: 06/12/06
Posts: 244
Re: Grow Log - First Try [Re: fromthemoon]
    #5977217 - 08/18/06 10:12 PM (17 years, 5 months ago)

casing. and cased.  not casting and casted :naughty:


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #5983776 - 08/21/06 12:36 PM (17 years, 5 months ago)

OK - everything is looking GREAT!  :smile:  No Contams that I can see.

Picture Time!

This is everything in the incubation chamber:


Liquid Cultures

This is the Blue Meanie - there was sediment and I'm not sure about this LC:


This is the Pink Buffalo, look a lot like the Blue Meanie:
Before Shakeing:


This is the PES Hawaiian - this looks good to me
Before Shaking:


After Shaking:


My LC lids:


Here are my 1/2 pint jars

Blue Meanie (slowest growth - inoculated 6 day ago):


Pink Buffalo (medium growth - inoculated only 4 days ago):



PES Hawaiian (Fast growth - inoculated 4 days ago):



I do not have pics of the quart jars yet, there is growth in those three too, but wouldn't show in a pic.

What do you guys think?


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Grow Log - First Try [Re: fromthemoon]
    #5983783 - 08/21/06 12:38 PM (17 years, 5 months ago)

looking like that LC is contaminated with bacteria.


should never look cloudy like that when shaken.


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Invisiblemagicbastard
I'm a teapot

Registered: 03/18/05
Posts: 791
Re: Grow Log - First Try [Re: monstermitch]
    #5983798 - 08/21/06 12:45 PM (17 years, 5 months ago)

the jars are looking alittle wispy they might be contaminated but wait till they turn green before tossing them


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OfflineOatman2000
-=Outa Space=-
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Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
Trusted Cultivator
Re: Grow Log - First Try [Re: magicbastard]
    #5983877 - 08/21/06 01:34 PM (17 years, 5 months ago)

after spinning my LC... it's cloudy. (plum full of Myc)

Tons of little hyphae broken up into ity bity pieces. :wink:


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Grow Log - First Try [Re: Oatman2000]
    #5983891 - 08/21/06 01:42 PM (17 years, 5 months ago)

yeah, but his isn't cloudy with mycelium.

in between the mycelium the liquid should still be crystal clear.
not in color, in clarity.

that to me looks like classic bacillus in an LC jar.

good cloudy:


bad cloudy:


--------------------



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OfflineOatman2000
-=Outa Space=-
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Loc: Planetary Nebula
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Trusted Cultivator
Re: Grow Log - First Try [Re: monstermitch]
    #5984326 - 08/21/06 04:52 PM (17 years, 5 months ago)

very good point indeed... only 1 way to find out for sure. Test it.

I know that these are bad and look at em .



not cloudy, but still bad. Everything sneeks up on ya in this hobby...


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Grow Log - First Try [Re: Oatman2000]
    #5984425 - 08/21/06 05:41 PM (17 years, 5 months ago)

LC's are stealthy.

it's very easy to contam one and not even know it.

all LC's should be tested on agar before use.


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: monstermitch]
    #5984484 - 08/21/06 06:00 PM (17 years, 5 months ago)

The LC's don't really look right to me either, but it's hard to tell with the honey water. I'm pretty sure the hawaiian is OK though.

Although the jars look like they have a ton of growth for just 4 to 6 days, I did grow a jar of cobweb before hand so I could see what it looked like. The growth in the jar doesn't look like cobweb. It's bright white and getting denser.

But...

These are multispore injections and the jars with the most growth got shot up with 2.5 to 3 ccs each. I'm waiting to see the think ropes form.

The Blue Meanies have the slowest growth, but showes the thicker ropes. I think the other jars are having a multispore issue with each other and I bet the thick ropes will then form.

This is a pic of my cobweb growth - This was early on and I let it grow much more:


My current jars don't look anything like the cobweb did.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #5984533 - 08/21/06 06:17 PM (17 years, 5 months ago)

Well now I'm not sure.

I didn't want to be a newb think spore growth was cobweb. But it reall doesn't look like cobweb - it's too thick and dark, even though there is some fluff. I looked at one of the Hawaiian jars, and the growth is cotteny, but bright white and there are tiny little ropes forming.

Honestly, I think the issue is multispore and once the different spore work things out, the ropes will get thicker, but the ropes are there.

Also, the LCs caramalized and had a ton of sedemit too.

Any more ideas?


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Extras: Filter Print Post Top
Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #5998849 - 08/26/06 11:54 AM (17 years, 5 months ago)

Update

Pics will come on mon/tue

While I'm waiting for my jars to colonize, I got bored and started a few new projects.

Project # 1

New glove box - the first one I build was kind of crappy (I didn't fully understand the concept), so I trashed it. I went to the container store and bought a clear plastic storage bin. Then I when to the hardware store and bought some rubber gaskets, clamps, gloves, and 2 part epoxy.

I cut two holes in the box, epoxyed the rubber gaskets to the holes and let it set. Then I attached the rubber gloves and epoxyed that and held with a clamp.

Worked great and much better than the other. The box is the top and the top lid is the bottom. With this glove box, I can spray down the box/gloves, apply a layer of bleach paper towels to the bottom, place my supplies in, and spray it down one more time with 50/50 bleach water before closing. Set aside for 10 minutes to overnight before working.

I'm going to look at adding a small hole so I can flame outside the box, but I want to take a little time before cutting into the best glove box I currently have.

Project # 2

New LCs - My first batch of LC was honey water and it caramelized pretty quickly and due to the observations of those jar (contaminated), I decided to make some new batches.

This time I got rid of my LC lid and went for the 2 holes covered with silicon and the polyfill syringe filter. Just seems easier to shake and handle without contams.

I used a different mix too. 1 tablespoon of light malt extract and 1 tablespoon corn sugar to 1 quart distilled water. I PCed for 15 min at 15psi.

They came out great with a little tiny amount of sediment. I inoculated them and it's beet between 1 and 2 days. I can actually see the little spores with white fibers coming out - germination.

They are incubating at 86.

Project # 3

Agar cultures - I wanted to test each syringe (and be able to test my LCs in the future), so I thought I would experiment with agar.

I could not find Petri dishes, so I used 10 small canning jars instead.

I made the agar with 9g agar agar (food grade) 10g light malt extract, and 500ml distilled water (I think that was how much water, but I followed the directions either way). I mixed that all up in a quart jar and double boiled it for 5 to 10 minutes until it was all dissolved. Then I poured it into each jar, sealed, and PCed for 1 hour at 15psi

I pulled them out of the PC hot and places in the glove box to sit overnight. The next day I took all of my syringes and labeled them. I added a few drops from each syringe to the agar and labeled the jars with the syringes.

There are 9 inoculated jars, 3 jars of each strain, 3 strains total. There is one un-inoculated jar as a control. They are also incubating at 86.

I'm going to keep an eye out for contams and good fast growth and try to isolate the best of the best and transfer to another agar jar and continue until I get a good fast growing strain.

I guess I'll have to also grow some out to know which agar plate fruits the best, but I'll cross that path when I get to it.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #5998856 - 08/26/06 11:56 AM (17 years, 5 months ago)

PS - my PK Jars so far have no contaims (that's out of 30 jars), but I'll know more on Monday and have pics.

My first LC's though, I don't know. I think contaminated, but I'll also know more on Monday


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Extras: Filter Print Post Top
Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #6024774 - 09/03/06 12:06 PM (17 years, 4 months ago)

Pics coming today.

There is so much to update, I'll have to split it up.

My first project was making syringes from 3 spore prints.  At this point, I can say that all three prints-to-syringes were NOT contaminated and completely vaible :smile: - not bad for a newb.

The next project were my LCs - in the first attempt, only one LC was healthy and viable (PES Hawaiian).  I felt this had to do with my original polyfill/tube/selfseal lid so I changed my lid to a 2 hole self-sealing lid w/ polyfill syringe filter.  I also changed from honey water LC to Malt Extract/Corn Sugar LC (much better IMHO).  I then inoculated those 3 new LCs - 2 grew successfully (Blue Meanie and Pink Buffalo).  The Hawaiian showed zero growth, but at least no contaims and it was the first PES Hawaiian LC that was successfull.  So now I have 3 successful LC, one of each strain.  I have inoculated a few jars and agar plates to test then as well.

New LC Pics:
Blue Meanie


Pink Buffalo


PES Hawaiian - no growth


PS - Very bad lighting, make the LCs look dark, but the myc is white)

The thrid project, and final from my original post, were my 30 1/2 pint jars inoculated with my multi-spore syringes.

5 of the Blue Meanie jars show zero growth - no myc and no contaims after 2-3 weeks.  The remaining 5 Blue Meanies are growing very slowly, but very ropey and healthy.

The PES Hawaiians grew very fast, 5 jars finished and are ready for birth today.  4 more need another week or 2.  1 jar developed a contaim (I remember watching a water droplet drain into one of the inoculation holes) which was kind of interesting since there was healthy myc on one side and cobweb on the other.  I wasn't sure at first, but after 2 days, the cobweb turned dark gray and there was a clear line between to the creatures.

The Pink Buffalos have been colonizing a little slow at first, but are now catching up to the hawaiians.  There are a few jars ready to birth, and a few that need another week or 2. 

Oh... I had three 100% quonia grain jars - 2 never colonized and the one that did is water logged - in the trash they went.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Edited by fromthemoon (09/03/06 01:28 PM)


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #6024797 - 09/03/06 12:15 PM (17 years, 4 months ago)

I did start other project while waiting for the first 30 jars to colonize.

I played with the alien tek, followed the directions (minus one cup of water - plus seaweed extract and 50% coffee water too), and filled several 1/2 pint jars and several quart jars and inocluated with my LCs to test.

I really like the alien tek so far.  It's a fun mix and so far is colonizing very fast (part from using LC, but also from the tek too).  Seems like the quart jars are colonizing just as fast as the 1/2 pints :smile:.

New Inoculation Jar Pics:
Quart Jar


1/2 pint jar




I also made the alien tek casing (spelled it right :wink:) and PC for 2 hours and have saved in there sealed jars for when ready.

I also PCed some New Zealand Moss (2 hours, dry and sealed) for the sphagnem moss tek.  Those are ready if and when I deside to try them.

Casing Mix Pic:


So all my casing materials are ready to go.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Edited by fromthemoon (09/03/06 01:33 PM)


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Offlinefromthemoon
ESP Major

Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #6024837 - 09/03/06 12:32 PM (17 years, 4 months ago)

And, I decided to play with Agar - tones of fun.

My first set of 9 agar agar/ malt extract jars germinated and have grown healthy white with no contaims. Again, PES Hawaiians germinated and covered the surface the fastest, followed by the Pink Buffalo. The Blue Meanies barely show germination and I might have to inoculate a new plate with the LC.

I went ahead and ordered 20 pre-poured malt agar plates, 40 grams of PDA, and glass slants for future storage.

I took my PESH and PB and cut a square from each jar, taking the best looking myc, and transfered it to a new agar plate. I'm not sure if this is right, but I placed 3 cut pieces from one original arag jar and place all three in one agar plate, each peice was placed equally apart. I did that because all the growth was fuzzy and I though I might be able to have better myc selection by seeing 3 growing next to each other. When I do the next transfere, I'll only place one per plate.

I am having a problem with condensation in the plates. Part of it has to do with the fact that it's too cold to leave them at room temp so they have to be incubated (tried room temp and growth stopped). The other reason is that the plates were sealed with micropore tape and that comes loose. I'll have to find some parafin tape ASAP. I think if the plates can rest on each other, the condensation with correct itself.

Agar Pics:
Bottoms:




Top:


But, so far so good - no contaims yet (but I better get that parafin quickly).


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Edited by fromthemoon (09/03/06 01:21 PM)


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Offlinefromthemoon
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Registered: 08/02/06
Posts: 353
Last seen: 16 years, 7 months
Re: Grow Log - First Try [Re: fromthemoon]
    #6024857 - 09/03/06 12:38 PM (17 years, 4 months ago)

Fruiting chambers

I build 3 fuirting chambers.

The first was from this tek - http://www.shroomery.org/index.php/par/3372 - Method 3: Using an Ultra Sonic Humidifier

I followed the tek exactly, but I think I will need to and an air bubbler for more FAE. I turned it on yesterday, so I'll be able to check the RH later today. It's a nice clean unit.

The second was inspired by the poor man's pod. It was a simple small bucket with a hole covered with thick plactic for light and perilite on the bottom. I think it worked well, but I didn't like having to fan all the time so I build a 1/2 martha.

Poor Man's Pod Pics:




I didn't have room for a full martha, so I bought a plactic storage container for hanging cloths. It's 25" X 25" x 55". I placed 4 shelves inside. I wired-up a cool mist and an ultrasonic and turned it all on. Look good, but I had soggy martha syndrom really quick and put the ultrusonic on a timer for 30 minutes on every 3 hours. It's not soggy anymore.

Martha Pics:




--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


Edited by fromthemoon (09/03/06 01:18 PM)


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