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Registered: 03/26/02
Posts: 14
Last seen: 21 years, 5 months
5 days, nothing
    #597516 - 04/03/02 10:39 AM (21 years, 8 months ago)

I have eight innoculated jars that have been incubating for five days, and as of yet I have seen no evidence of mycelium growth. I am using a fish tank heater inside a large plastic cup for heat. With the thermostat on the heater turned up to ninety-two degrees, the highest temperature I can get is about seventy-six degrees. I know this is below optimal temperature, but I was wondering if this could be what is causing the delay.

Also, when mixing my substrate I used tap water, not distilled water, as the MMGG did not suggest one way or the other. I boiled the jars once, and forgot to put a lid on the pot I was using. So I boiled them again, this time with the lid in place. Did this dry them out too much? If so, is there a chance that the jars could fully colonize and I could case them to restore proper moisture?

I have been routinely checking the jars, and have noticed no contamination. Do you think they might be OK and I shouldn't worry yet? Have I made any grave errors and sabotaged my project before it even got off the ground? Any advice/questions/comments/rude remarks would be greatly appreciated.

"Fireflies bring the stars down to us" -Jonathan Greene
"Gravity is a myth- the world sucks" -Chad M. Horn

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Error: divide byzero

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Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 2 days
Re: 5 days, nothing [Re: monkeylove]
    #597521 - 04/03/02 10:45 AM (21 years, 8 months ago)

Give them up to two weeks before you start to worry. My friend has never had it take more than two weeks for germination to occur, but I have read that it can take up to a month with old spores.

Just another spore in the wind.

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Re: 5 days, nothing [Re: Seuss]
    #597636 - 04/03/02 12:47 PM (21 years, 8 months ago)

I had some hawaiins that took 10 days before I noticed anything at all. All my other strains were finished by the time they just started, but once they did they took off and were finished in a week.

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Registered: 03/20/02
Posts: 29
Last seen: 21 years, 6 months
Re: 5 days, nothing [Re: monkeylove]
    #597781 - 04/03/02 03:58 PM (21 years, 8 months ago)

Monkey, if you want to get the optimum temp, you could get a plastic rubbermaid container (or something like it) and put some water in it, and set the jars in with the heater in the water set on 85 or so. I did that, and the jars just took off :-) Good luck

"There are no passengers on spaceship earth. We are all crew."

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Brian Eno is mypersonal God FNORD

Registered: 02/18/02
Posts: 423
Loc: ACT, Australia
Last seen: 6 years, 1 month
Re: 5 days, nothing [Re: monkeylove]
    #597801 - 04/03/02 04:17 PM (21 years, 8 months ago)

Patience young grasshopper.  76?F is a bit cool for the spawn run (best 84-86?F.)  It may take a bit longer.  Also if the spores were old then it may take a bit longer.  The tap water shouldn't make a difference. Just keep your nose peeled for any odd smells, if there is any bacterial contamination, you'll smell it before you see it. 
:smile: Good Luck! :smile: 

MushyMay is a fictional character, as a result any information provided by MushyMay is also fictitious.

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