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OfflineWiggly
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half pint not enough
    #5970720 - 08/16/06 07:40 PM (17 years, 5 months ago)

ok I got a ecuador syringe on the way. this is supposed to be enough to inoculate 12 half pint jars right?

Could I just double up my mixture and do 12 full pint jars with that one syringe? (from sporesource) A half pint is kinda small after looking around for supplies today imo.

also, whats the proper way to store a syringe? and how long do they last?

Where do you guys get vermiculite? I couldnt find it at 2 wallmarts OR target...

Is "all natural" brown rice ok? or does it have to say organic?

sorry Ive read all this before but am not 100 percent sure on some of these and just double checking. thanks -kris


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Just learnin to cultivate.


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InvisibleTippinthru
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Re: half pint not enough [Re: Wiggly]
    #5970739 - 08/16/06 07:46 PM (17 years, 5 months ago)

Think LC


Use 2 ml of spore syringe to make a 250 ml LC. :grin:

Save the rest of the spore solution for later.  :tongue:


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Perfection is attained by slow degrees; it requires the hand of time...
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OfflineWiggly
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Re: half pint not enough [Re: Tippinthru]
    #5970742 - 08/16/06 07:48 PM (17 years, 5 months ago)

this is gonna sound noobis but... LC?

Whats that pic of?  I'm just trying to make my first cakes to get some hands on experience... then casings... then experimenting with different casings, depths, strains, etc etc. :smile:


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Just learnin to cultivate.


Edited by Wiggly (08/16/06 07:50 PM)


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InvisibleRahz
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Re: half pint not enough [Re: Wiggly]
    #5970764 - 08/16/06 07:55 PM (17 years, 5 months ago)

Skimping on innoculant in BRF jars isn't a good idea.

IMO, using pint jars for BRF is a bad idea, they're more likely to stall out.

Store syringes in the fridge, preferably in an airtight container. They're good for at least a year, mabey 2-3.

For verm, check Lowes or Home Depot. If that fails, look for a garden supply store. If you've got storage space, the garden supply places usually carry 4 cubic foot bags for 10-15 bucks. It's like buying it for pennys on the dollar.

All natural should be the same as organic, though I don't think the rice needs to be certified organic.


--------------------
rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi


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OfflineA0999
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Re: half pint not enough [Re: Wiggly]
    #5970775 - 08/16/06 07:56 PM (17 years, 5 months ago)

Quote:

ok I got a ecuador syringe on the way. this is supposed to be enough to inoculate 12 half pint jars right?




there will prolly be 10ml in the syringe. most people reccomend usind 1ml per half pint. you could stretch it to 12 w/o any problems though.

Quote:

Could I just double up my mixture and do 12 full pint jars with that one syringe?




i suggest you not try this unless your syringe is like BLACK. otherwise pints jars will take too long to colonize and prolly cause problems.

seeing as you are receiving a vendor quality syringe i would think there would be a minimal spore count.


Quote:

A half pint is kinda small after looking around for supplies today imo.




12 half pints will produce plenty of fruit, especially for your first grow. optimal conditions provided.

Quote:

also, whats the proper way to store a syringe? and how long do they last?




you can keep them in the fridge, a drawer, storage container, or anwhere that is dark, dry, and not too hot. they will last a long while like that.

Quote:

Where do you guys get vermiculite? I couldnt find it at 2 wallmarts OR target...




try nurseries and garden stores.

Quote:

Is "all natural" brown rice ok? or does it have to say organic?




organic probably.


--------------------
Not necessarily stoned, but beautiful


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Invisiblecreamcorn
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Re: half pint not enough [Re: A0999]
    #5970854 - 08/16/06 08:14 PM (17 years, 5 months ago)

organic = scam. :smile:  people think organic means no pesticides/fungisides... not so... they just use different ones that the FDA says are ok to still be certified. :wink:  they also think it means higher quality... again, not so.  so whats the point?  to bilk money out of hippies :thumbdown:

any "no preservative" brown rice/brown rice flour, is plenty fine. 

and what does the darkness of a syringe have to do with anything?  12 jars worth of innoculations is 12 jars worth of innoculations, regardless the size of the jar...  there's enough spores to go around in a crystal clear syringe to do the job.  don't make it sound as if there's a problem with "vendor quality" syringes or that "black" syringes are best, because sorry, that's just not valid reasoning.

anyway.  stay away from pints.  they do work and you can use them.  they need to be prepared very well so that they colonize before other problems like moisture loss or contamination sets in.  they will take longer than half pints as well.  stick with what works, and whats likely to work best given your inexperience... the half pinters. 

don't get so hung up on yields in the beginning, you've got a long way to go before you're harvesting a damned thing, so be glad when you get something, then work on improving from there if you're not satisfied with the amount :smile:


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Offlinefirstcut
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Re: half pint not enough [Re: creamcorn]
    #5970920 - 08/16/06 08:27 PM (17 years, 5 months ago)

what happend to "eatyualives lazy mofo bag tek" i cant seem to get that link back up but it sounded gr8. U take a colonized jar and mix it with 3 other pc jars in a bag


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The are no stupid questions, only stupid people, now point and laugh every one.


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OfflineWiggly
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Re: half pint not enough [Re: firstcut]
    #5970948 - 08/16/06 08:32 PM (17 years, 5 months ago)

wow thanks guys for all the speedy replies, MUCH appreciated. I'll take everything you guys said into consideration! -kris

ps. I'd be passing out ratings if I could, but I dont have 25 posts yet lol


--------------------
Just learnin to cultivate.


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OfflineWiggly
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Re: half pint not enough [Re: Wiggly]
    #5970992 - 08/16/06 08:41 PM (17 years, 5 months ago)

whats FAE... search shows things like when is best to do FAE etc etc... but what IS it?


--------------------
Just learnin to cultivate.


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OfflineCubenisseur
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Re: half pint not enough [Re: Wiggly]
    #5971017 - 08/16/06 08:45 PM (17 years, 5 months ago)

Fresh Air Exchange. Very important for promoting fruiting.

Also, don't ignore the LC(liquid Culture) that Tippi pointed out. Your one syringe could innocualte hunders of jars if you wanted....Read up on it!


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OfflineA0999
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Re: half pint not enough [Re: creamcorn]
    #5971106 - 08/16/06 09:06 PM (17 years, 5 months ago)

Quote:

creamcorn said:
and what does the darkness of a syringe have to do with anything? 12 jars worth of innoculations is 12 jars worth of innoculations, regardless the size of the jar... there's enough spores to go around in a crystal clear syringe to do the job. don't make it sound as if there's a problem with "vendor quality" syringes or that "black" syringes are best, because sorry, that's just not valid reasoning.




1ml of a "black" syringe would containt many more spores than 1ml of a clear syringe.

can you not see how it would be more benificial to inject a jar with 1ml of the darker syringe?


--------------------
Not necessarily stoned, but beautiful


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Offlineghostofbmarley
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Re: half pint not enough [Re: A0999]
    #5972124 - 08/17/06 05:18 AM (17 years, 5 months ago)

Sporesource usually sends out 12cc syringes (BTW).

Verm can be a bit tough to find. Ace Hardware / True Value / Lowes. I have seen it regularly at all 3.

I would seriously consider using the entire syringe to get your 12 cakes going and focus on LC during your incubation. If you can get away with having 1CC left over to work on LC, great, but I would concentrate on the success of your first cakes for now. During incubation you'll have lots of time for add'l research. GL!

-ghost


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~OM MANI PADME HUM~


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InvisibleFooMan
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Re: half pint not enough [Re: Wiggly]
    #5972129 - 08/17/06 05:28 AM (17 years, 5 months ago)



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OfflineCosmicFunGuy
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Re: half pint not enough [Re: FooMan]
    #5972365 - 08/17/06 09:39 AM (17 years, 5 months ago)

I think you should use 2 cc's in some karo or honey water to make an LC, i would advise reading up on them (liquid cultures). You could repeat this grow a hundred times with an LC, all off your original 2 cc's of multi spore solution. (think about it if all your jars get contaminated you'll have all the solution you need to knock up a jar again AND this is a bonus, a Jar knocked up with LC will colonize faster than one knocked up with multispore solution. This doesn't mean better mycelium just faster because in an LC the spores germinate so when you knock up the jars you don't have to wait for germination just the mycelium to grow. If you do go with an LC, and might I add it is really easy to do, use the first two cc's off your syringe this leaves less room for it to get contaminated a lot of first timers have issues with sterility and don't realize it until too late. the first use of a sterile syringe is almost sure to be clean unless you take the cap off the needle and set it on the counter or something stupid. every time after the first jar that you go to inoculate there are more chances of contamination if your sterility procedures aren't dead on. My first indoor grow the first 5 out of 18 jars that i knocked up took off and never looked back but the other 13 all contamed. WHY? because i wasn't using a proper sterility technique. If you use your first 2 cc's for the LC and you do everything properly you won't have a thing to fret about if you get contamination in a BRF jar, just have to make new jars and knock it up with LC.

I've never tried pint's but some really experienced people have suggested against it and said stick to half pints I agree if you are going to do the BRF cakes stick to half pint, they take long enough to colonize i wouldn't want to wait for a pint. All that time seems like asking for contamination and stalled jars.


As for people talking about black syringes better than the crystal clear ones... Well... IT'S CRAP! Let's put that idea into the ground right now. hope I could be of help...


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♫ ♪ In the garden of many fields, there is no me or you. There is no right or wrong. There is no up or down. There is no black or white. Everyone is the plow man. Everyone is the seed. Everyone harvests and everyone yields. In the garden of many fields, everyone is one with one love and one love is one with everyone. ♪ ♫
lyrics from: Transglobal Underground - Eyeway Souljah (from Psychic Karaoke)


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InvisibleRoadkillM
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Re: half pint not enough [Re: A0999]
    #5972422 - 08/17/06 10:19 AM (17 years, 5 months ago)

Quote:

anarchist0999 said:

Quote:

creamcorn said:

and what does the darkness of a syringe have to do with anything?  12 jars worth of innoculations is 12 jars worth of innoculations, regardless the size of the jar...  there's enough spores to go around in a crystal clear syringe to do the job.  don't make it sound as if there's a problem with "vendor quality" syringes or that "black" syringes are best, because sorry, that's just not valid reasoning.




1ml of a "black" syringe would containt many more spores than 1ml of a clear syringe.

can you not see how it would be more benificial to inject a jar with 1ml of the darker syringe?




A dark syringe is not better than a clear or light colored one...
spores are microscopic and cannot be seen by the human eye...
unless there is a crap load or clumping of spores in the syringe.

Using a dark syringe will cause too many spores to germinate...
which will might give you quicker colonization times in some cases...
but will lead to other problems.

Too many substrains...and they are all competing for the substrate.

RR can explain it better than me.
:smile:

I like using a spore syringe with just a tad bit of color to it...
I can get full colonization in 7 to 10 days from a multi-spore inoculation.
Even faster from an LC.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineA0999
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Re: half pint not enough [Re: Roadkill]
    #5972439 - 08/17/06 10:27 AM (17 years, 5 months ago)

just speaking from experience. i have known it to work better MANY times.

never had any problems, always good results


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OfflineDnBMiXer
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Re: half pint not enough [Re: A0999]
    #5972461 - 08/17/06 10:34 AM (17 years, 5 months ago)

i had the same problem finding verm

dont let the assholes at home depot tell you that perlite is the same as verm. I mean it is as far as they're concerned but they don't know what your intentions are and even if they did they would still tell you its the same thing.

I finally found my verm. at southern states. but I had to go to the big main store with the warehouse in the back. it was 4 cu. ft. bag for $17


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Invisiblecreamcorn
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Re: half pint not enough [Re: A0999]
    #5972483 - 08/17/06 10:45 AM (17 years, 5 months ago)

Quote:

anarchist0999 said:
1ml of a "black" syringe would containt many more spores than 1ml of a clear syringe.

can you not see how it would be more benificial to inject a jar with 1ml of the darker syringe?




no, i don't... in fact, its wasteful. in theory it only takes 2 spores to colonize a jar and produce fruits. in practice, injections from even the clearest of syringes will result in hundreds of spores being introduced into the jar. its been my experience consistently that fewer spores result in more agressive growth, because there's fewer substrains vying for the same resources. in my opinion, the only thing a dark syringe is good for, is diluting it into several lighter ones.


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OfflineA0999
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Re: half pint not enough [Re: creamcorn]
    #5973681 - 08/17/06 05:42 PM (17 years, 5 months ago)

Quote:

creamcorn said:
its been my experience consistently that fewer spores result in more agressive growth






the only reson i even mentioned using dark syringes is because my resuslts have been the complete opposite of yous apparently.


i will agree with the competing substrains. however, allthough i have not touched BRF, or used a spore syringe for anything other than LC in over 2 years i can recall that my cakes did much better than almost all others i have seen around here.

i guess il take you guys' word for it though.


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OfflineWiggly
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Re: half pint not enough [Re: A0999]
    #5974546 - 08/17/06 10:02 PM (17 years, 5 months ago)

Thank all you guys SO damn much for all the info.  I'll will be on my first attempt sometime next week.  I know I've only been reading for a few weeks, but I'm confident that I will be successfull. Expect a complete grow log a few months from now :smile: .  I think I will try to make a LC jar when my syringe gets here next week for sure.  I can spare 4 jars to make many many for if I do it correctly.  I'm looking over a couple of LC making teks as we speak.  Again, thanks for your wealth of knowledge! -kris


--------------------
Just learnin to cultivate.


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