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the spiral
Neuroscientist



Registered: 05/13/02
Posts: 1,769
Last seen: 8 years, 9 months
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Agar plates & ciprofloxacin/penicillin
#5970409 - 08/16/06 06:22 PM (17 years, 9 months ago) |
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Hey, and thanks for reading my post.
A friend of a friend is going to be culturing cubensis on agar plates, and wants to add antibiotic to his malt agar solution in order to prevent bacterial contamination. He currently has in his posession about a dozen ciprofloxacin pills, and he thinks he might be able to dig up penicillin somewhere.
My friend of a friend was wondering if it would work to simply use some powderized cipro pills to add to his agar solution (before sterilizing), and how much to use (milligrams of cipro per liter of malt agar solution, for example)
Thanks for your input! My friend much appreciates your help
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  "A celibate clergy is an especially good idea, because it tends to suppress any hereditary propensity toward fanaticism." - Carl Sagan
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curenado
73rd Man


Registered: 04/01/03
Posts: 2,662
Loc: North Central Arkansas
Last seen: 1 day, 55 minutes
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Re: Agar plates & ciprofloxacin/penicillin [Re: the spiral]
#5973102 - 08/17/06 02:33 PM (17 years, 9 months ago) |
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Typically gentamycin is used in agars and in very small amounts.
Suntzu has said: <<Ampicillin is cheap; I use 50 ug/mL. Tetracycline, I don't know what is optimal, but 30 mg/mL works. [both post-sterilization, of course].>>
Best wishes!
-------------------- Yours in the Natural State Land of Enchantment! "The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...." "When psychotomimetics become cultural, so does cultural psychosis"
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Agar plates & ciprofloxacin/penicillin [Re: curenado]
#5973302 - 08/17/06 03:47 PM (17 years, 9 months ago) |
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Gentamycin sulphate is the only antibiotic I know of that works well with agar. It is added BEFORE sterilization at the rate of 1/15 gram per liter. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Workman
1999 Spore War Veteran


Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
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Re: Agar plates & ciprofloxacin/penicillin [Re: the spiral]
#5974159 - 08/17/06 08:15 PM (17 years, 9 months ago) |
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Most antibiotics are heat sensitive and are inactivated by sterilization. Gentamycin is the exception and can be added before cooking. If you wish to use most other antibiotics, they have to be added after sterilization and after the agar has cooled siginificantly (but still liquid). Since the pills are not likely sterile, you would probably be adding more contamination than you would be preventing.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
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Re: Agar plates & ciprofloxacin/penicillin [Re: Workman]
#5975287 - 08/18/06 07:03 AM (17 years, 8 months ago) |
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Some other antibiotics that are not rendered completely useless due to sterilization are penicillin and/or stroptomycin.
See this post from 2003: http://www.shroomery.org/forums/showflat.php/Number/1223042
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Pinback
Stranger


Registered: 07/20/02
Posts: 836
Loc: Europe
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Re: Agar plates & ciprofloxacin/penicillin [Re: Anno]
#5975298 - 08/18/06 07:14 AM (17 years, 8 months ago) |
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Chloramphenicol is also stable to high temperatures, as indicated here. Note however that it is bacteriostatic, and not bacteriocidal.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Agar plates & ciprofloxacin/penicillin [Re: Pinback]
#5988732 - 08/23/06 04:32 AM (17 years, 8 months ago) |
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I've had very mixed results adding random antibiotics in random amounts to liquid cultures. I don't really recommend it unless you're just experimenting.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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mycogirl
goddamn



Registered: 07/03/05
Posts: 1,135
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Re: Agar plates & ciprofloxacin/penicillin [Re: fastfred]
#5992506 - 08/24/06 12:10 PM (17 years, 8 months ago) |
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Unless the antibiotic is specifically culture grade (which oral antibiotics are not), you will have variable results. The easiest way to avoid contamination is good technique.
In addition, tsk for not taking your antibiotic until it was gone. This is bad for more reasons than I have time to list.
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curenado
73rd Man


Registered: 04/01/03
Posts: 2,662
Loc: North Central Arkansas
Last seen: 1 day, 55 minutes
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Re: Agar plates & ciprofloxacin/penicillin [Re: mycogirl]
#6038498 - 09/07/06 01:32 PM (17 years, 8 months ago) |
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<<The easiest way to avoid contamination is good technique.>>
That's all we do and we do pretty good! Your tech and your clean box are your best defenses.
-------------------- Yours in the Natural State Land of Enchantment! "The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...." "When psychotomimetics become cultural, so does cultural psychosis"
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