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InvisibleBrillig
Prodigal Son

Registered: 02/16/02
Posts: 236
Stalled Jars
    #5967399 - 08/15/06 08:04 PM (17 years, 5 months ago)

Guys...I've been at this for several years and I've never had this happen before, but my Treasure Coast jars have completely stalled...No contams...but no growth for weeks...I just started them for fun a few months ago...I had some mycelium growing on agar and I had some brown rice in the house, so I made a few jars and inoculated them with the agar...At first the growth was rapid and encouraging, but after a few weeks it slowed and basically stopped...I've waited so long, I kinda hate to lose them...Any ideas what I can do???


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My father started me on this hobby over 30 years ago...I ask  beginner's questions whenever they occur to me...Usually to stir the pot and get people's thoughts...When I post a REPLY...IT SHOULD NOT BE ACCEPTED AS GOSPEL TRUTH...It's just what I THINK...

PEACE ALL!!!


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Offlineimtank
Self-Taught Fool
Male

Registered: 07/05/06
Posts: 235
Loc: somewhere around Barstow,...
Last seen: 16 years, 2 months
Re: Stalled Jars [Re: Brillig]
    #5967608 - 08/15/06 08:57 PM (17 years, 5 months ago)

they usually stall becuase of contam, you just might not be able to see it...how far along colonized are they??? what do they smell like?


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'To be positive at all times is to ignore all that is important, sacred or valuable. To be negative at all times is to be threatened by ridiculousness and instant discredibility.'
-Kurt Kobain


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InvisibleBrillig
Prodigal Son

Registered: 02/16/02
Posts: 236
Re: Stalled Jars [Re: imtank]
    #5968895 - 08/16/06 09:03 AM (17 years, 5 months ago)

Yeah...My sniffer is actually broken right now...can't really smell much, but it smells sweet, possibly fermented, but no obvious signs of contam...I'm guessing the sweet/ferment smell could be from the agar, but it's my first time experimenting with it...

If I were to birth, crumble and case (removing as much of the uncolonized, brown rice as possible) how much of a contam risk am I taking???


--------------------
My father started me on this hobby over 30 years ago...I ask  beginner's questions whenever they occur to me...Usually to stir the pot and get people's thoughts...When I post a REPLY...IT SHOULD NOT BE ACCEPTED AS GOSPEL TRUTH...It's just what I THINK...

PEACE ALL!!!


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Offlinetonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
Re: Stalled Jars [Re: Brillig]
    #5968912 - 08/16/06 09:09 AM (17 years, 5 months ago)

go for it, you got nothing to lose at this point IMO

Just try to use it as a learning experience if you figure out why and let us know  :wink:


Edited by tonyperez420 (08/16/06 09:10 AM)


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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Stalled Jars [Re: Brillig]
    #5968985 - 08/16/06 09:52 AM (17 years, 5 months ago)

you need to explain what tek or teks you are following...

and why are you using agar with brown rice?

what method of inoculation did you use?


we need more information to help you!~


tc


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflinePsiloptile
a legal alien
Male User Gallery

Registered: 06/15/06
Posts: 125
Last seen: 15 years, 8 months
Re: Stalled Jars [Re: Roadkill]
    #5969059 - 08/16/06 10:26 AM (17 years, 5 months ago)

One of my jars stalled out for about a week and then picked up again after a stint in the ICU, which is basically a towell-wrap.. Took about twice as long to fully colonize as the rest o them, but it happened in the end. What's your temp setting? What's your substrate Mix?


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Everything I write is false.. I made it up.. Or did I? Yep.. I did.



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Invisiblecreamcorn
mad scientist
Male User Gallery

Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: Stalled Jars [Re: Brillig]
    #5969145 - 08/16/06 11:03 AM (17 years, 5 months ago)

Quote:

Brillig said:
Yeah...My sniffer is actually broken right now...can't really smell much, but it smells sweet, possibly fermented, but no obvious signs of contam...I'm guessing the sweet/ferment smell could be from the agar, but it's my first time experimenting with it...

If I were to birth, crumble and case (removing as much of the uncolonized, brown rice as possible) how much of a contam risk am I taking???




like RK said, why is there agar in your BRF jars?  agar belongs in petri dishes when doing culture work.  there's not much purpose or benefit to try and use it for anything else.... its basically like jell-o, there's no function of having jell-o in a PF jar. :smile:  it will only screw up the texture and airiness of a substrate jar. 

anyway... its irrelevant, your jars are done for.  sweet/fermented means they've been infected with bacteria... that is an obvious sign you should look out for.  they won't look different really, they might appear a darker shade of brown... sometimes the edges of the mycelium that was colonizing will thicken up and take on a yellow/brownish color if left for a little while.  colonizing PF jars shouldn't really smell like anything.  when you open to birth, they smell like fresh mushrooms, but that smell isn't strong enough to permeate the verm layer and make it through your holes... they should be scentless basically when they're sealed up and colonizing.  agar doesn't have a strong enough odor that you'd notice it outside the jars either. a bacterial infection will stop colonization dead in its tracks.  considering your jars stalled, and they smell, i can promise you with near 100% certainty they're contaminated, and there's no saving it once bacteria hits.  if you crumble & case at this point, they're not going to go anywhere, and will only smell worse, and other contams will quickly move in because the mycelium is either weak or dead at this point.  sorry to be the bearer of bad news  :frown:


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