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TrippinOnAcid
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Registered: 08/03/06
Posts: 226
Last seen: 14 years, 1 month
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Sterilize Straw w/ out Boiling?
#5964134 - 08/14/06 08:46 PM (17 years, 5 months ago) |
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Edit: I will have access to a stove but only for a short period of time ... is there any way to store the pasterized straw until my WBS cakes are done (which will be started at the same time I have access to a stove)???? If not then I need an alterinitive method as stated below.
I want to follow ShroomGods Straw Tek, http://www.shroomery.org/index.php/par/7937
but there is one thing that I dont think I will be able to achieve.
"Cook at 160-180°F for 1 to 2 hours"
What other ways can I sterilize my straw but still be as effective as the reccomened method above?
I did find another tek that showed some other ways...
"Lime water treatment: Fill a clean, 45 gallon drum with fresh water and add 4 cups of Calcium hydroxide (hydrated lime) or Calcium oxide (quicklime) with stirring. Prepare straw as for hot water pasteurization above, then completely immerse in the lime water solution for about 2 hours. The water need not be hot, but very cold water does not seem to work well. Remove the bags to a clean place to drain, then spawn as for hot water pasteurized straw. This method is very economical as lime is cheap, but it is mild, and only good quality, clean straw should be used. Lime water will not kill grains, and sprouting grain may promote certain microbial pests. One batch of lime water will treat about 20 Kg. of straw.
Hypochlorite treatment: Add 4 cups of household bleach solution to 45 gals. water to prepare a disinfectant solution that works about as well as lime water and is very economical. Immersion time and limitations are as for lime water. All the straw must be immersed at once. Serial treatment of a number of batches does not work well as the hypochlorite becomes depleted.
Formaldehyde and maneb treatment: A recent article Mush.J.Tropics 7:67-75 (1987) describes an effective method for sanitizing wheat straw by immersion in dilute formaldehyde (500 ppm)/Maneb (75 ppm) solution. To prepare the solution, add 1 cup of 37% Formalin solution and 1/4 tsp. Maneb powder with stirring to 45 gals water. Immerse chopped straw in the above solution for 18 hours, drain and spawn as above. Zineb or Benomyl might substitute for Maneb in the same application.
Cold water immersion: Fill a tub with clean, cold water. Prepare straw as for hot water, and completely immerse in the water for 5-7 days. The water should have a large surface area and have some opportunity to circulate by convection, to avoid anaerobic conditions. Use good quality straw. Supplementation prior to immersion is not recommended. At the end of the immersion period, remove the straw from the water, drain and spawn as above."
What would be the most effectice way? Is boiling even the best way to do it? I want to have the best results and dont want my straw to be contaminated but I also dont think boiling it will be a possiblity.
Thanks
Edited by TrippinOnAcid (08/14/06 08:48 PM)
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Sterilize Straw w/ out Boiling? [Re: TrippinOnAcid]
#5964164 - 08/14/06 08:56 PM (17 years, 5 months ago) |
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If you sterilize straw, it will contaminate before you can get it colonized. If you don't have a way to pasteurize it, then I suggest you just stick to the brf cakes as you learn the ropes. I've never had much success with chemical pasteurization. I've had the best results by washing the straw with dish soap and water, then pasteurize for an hour at 140F to 150F with one cup of hydrated lime added for each ten gallons of pasteurization water. Proper pasteurization will give you a two week window of opportunity to get the straw colonized to the point where the mushroom mycelium can hold its own against the contaminants. Good luck. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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