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OfflineBabo911
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Strand isolation with peroxide tek
    #5961917 - 08/14/06 06:12 AM (17 years, 6 months ago)

Since you can not put spores onto peroxide agar, could you make a small grain jar, knock that up and use a single colonized grain on your plate for making an isolated strand?


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Ronhero on the Growery is me


Edited by Babo911 (08/14/06 06:15 AM)


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Offlineroyer
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Re: Strand isolation with peroxide tek [Re: Babo911]
    #5961918 - 08/14/06 06:15 AM (17 years, 6 months ago)

yep i have done that with wbs. works great.


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OfflineBabo911
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Re: Strand isolation with peroxide tek [Re: royer]
    #5961919 - 08/14/06 06:16 AM (17 years, 6 months ago)

cool, I have not been able to get a good agar dish going since i blew up my glove box :smile:


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Offlineroyer
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Re: Strand isolation with peroxide tek [Re: Babo911]
    #5961934 - 08/14/06 06:43 AM (17 years, 6 months ago)

i dont use h2o2 tho after i am done putting my wbs grain on the dish i press it down lightly and then spray the top of the petri with lysol shake it then put the lid on and wrap it in cling wrap never had a contam yet :-)


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OfflineRogerRabbitM
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Re: Strand isolation with peroxide tek [Re: royer]
    #5962080 - 08/14/06 09:05 AM (17 years, 6 months ago)

What is an isolated strand? If you meant to say isolated strain, that isn't how it's done. You want to isolate many strains so you can fruit them independently to find the best performer. That means starting from spores, then isolating the various zones of separate growth that appear on the petri dish away from each other, so that each can grow out on its own dish. Don't use peroxide. Instead, use sterile procedure.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineroyer
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Re: Strand isolation with peroxide tek [Re: RogerRabbit]
    #5962087 - 08/14/06 09:11 AM (17 years, 6 months ago)

i think what he is talking about is starting a multispore grain on a petri then doing some strain isolation trasfers till he gets an isolated strain. then from there he is going to see if it is a fruiting strain and make an lc out of it. right

but he just wantd to know if he could use a piece of grain to start his dish..instead of spores.


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Offlinebeatnicknick
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Re: Strand isolation with peroxide tek [Re: royer]
    #5962131 - 08/14/06 09:35 AM (17 years, 6 months ago)

Hahah an isolated strand. This guy has to be like 2.


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OfflineBabo911
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Re: Strand isolation with peroxide tek [Re: royer]
    #5962173 - 08/14/06 09:54 AM (17 years, 6 months ago)

Quote:

royer9864 said:
i think what he is talking about is starting a multispore grain on a petri then doing some strain isolation transfers till he gets an isolated strain. then from there he is going to see if it is a fruiting strain and make an lc out of it.  right

but he just wantd to know if he could use a piece of grain to start his dish..instead of spores.




Right on!

The reason being is that on peroxide agar you can not put spores, only live tissue or such.

And I am not 2, just not the best speller that is all :smile:


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Offlinebeatnicknick
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Re: Strand isolation with peroxide tek [Re: Babo911]
    #5962178 - 08/14/06 09:55 AM (17 years, 6 months ago)

Just yanking your balls. When the opportunity is presented, I have a tendency to yank.


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I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.


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Invisiblefyouseeku
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Registered: 05/25/06
Posts: 79
Re: Strand isolation with peroxide tek [Re: beatnicknick]
    #5962182 - 08/14/06 09:58 AM (17 years, 6 months ago)

Quote:

beatnicknick said:
Hahah an isolated strand. This guy has to be like 2.




What a bitch! :ass2mouth:


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Offlinebeatnicknick
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Re: Strand isolation with peroxide tek [Re: fyouseeku]
    #5962199 - 08/14/06 10:05 AM (17 years, 6 months ago)

I'm not even a girl dog, that's just a cover up so the DEA arrests my girl when they hunt down all of the shroomerites.

Anyway this is way off topic sorry for interfering your discussion here.


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I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.


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Invisiblemonstermitch
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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Strand isolation with peroxide tek [Re: beatnicknick]
    #5962489 - 08/14/06 11:53 AM (17 years, 6 months ago)

Quote:

beatnicknick said:
I'm not even a girl dog, that's just a cover up so the DEA arrests my girl when they hunt down all of the shroomerites.





yeah, I see that working.

however, it does speak very clearly to your character.

:thumbdown:


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Offlinebeatnicknick
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Re: Strand isolation with peroxide tek [Re: monstermitch]
    #5962546 - 08/14/06 12:14 PM (17 years, 6 months ago)

That was a joke by the way, I live in a dorm alone.  :rolleyes:


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I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.


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OfflineFriendlyNeighbor
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Re: Strand isolation with peroxide tek [Re: monstermitch]
    #5962550 - 08/14/06 12:17 PM (17 years, 6 months ago)

Is there pure agar agar out there? The agar mix usually contain sugar in the mix. Is that alright to use for isolating strains in petri dishes?


Edited by FriendlyNeighbor (08/14/06 12:18 PM)


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