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Shop: Mushroom-Hut Liquid Cultures, Substrate Mix   North Spore Bulk Substrate, Injection Grain Bag

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Offlinefaceyneck
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Liquid cultures and dunking
    #5961997 - 08/14/06 07:55 AM (17 years, 5 months ago)

I don't know from experience, but I read here that a fully colonized cake dunked in water for much more than 24 hours will die, yet a liquid culture will stay viable for months in the fridge. Why does the mycelium in the liquid culture live, and the dunked cake die?


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Invisiblecreamcorn
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Re: Liquid cultures and dunking [Re: faceyneck]
    #5962100 - 08/14/06 09:19 AM (17 years, 5 months ago)

A dunked cake won't really die after 24 hours. I've dunked for 4 days at one time and had absolutely no problem...

Excessive dunking can waterlog the cake, or damage it "structurally" making it a pile of mush if you dunk it too long.

More importantly, its more likely that bacteria will overrun the cake before the cake will "drown" - bacteria can swim in water and multiply very quickly. Even under refrigeration if left too long they can begin to accumulate in the dunk water. Bacteria shouldn't be a problem on the other hand within a culture jar, because the contents within should be sterile.

Mycelium cultures are stored on the long term in distilled water, and they certainly don't "die" in there either.


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Offlinebeatnicknick
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Re: Liquid cultures and dunking [Re: creamcorn]
    #5962163 - 08/14/06 09:50 AM (17 years, 5 months ago)

Hmm. That gives me an idea of a liquid culture method. First, a pot of water should be boiled to sterilize some water. Take some of the water and fill up a sterilized zip lock bag of something that can hold the water, and through in your most sterile cake. Pour in some peroxide for good measure- after all, your cake does have bacteria on it no matter what. You just want there numbers to be low. Let soak for a few day until its mushy, squish it around until its nice an liquid cultery, and stick your syringe in there and suck up as much mycelium and as little verm as possible.

The thing is, I don't think you could repeat this process due to the fact that the mycelium (I've heard) will just bum out and crap out little turd like flushes.


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Offlinefaceyneck
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Re: Liquid cultures and dunking [Re: beatnicknick]
    #5962328 - 08/14/06 10:50 AM (17 years, 5 months ago)

"The thing is, I don't think you could repeat this process due to the fact that the mycelium (I've heard) will just bum out and crap out little turd like flushes."

By "repeat this process," do you mean inoculating a substrate with the suishy LC, and then taking the new substrate and making another squishy LC for more substrate, etc.?


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Invisiblecreamcorn
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Re: Liquid cultures and dunking [Re: faceyneck]
    #5962340 - 08/14/06 10:53 AM (17 years, 5 months ago)

it could work... i think theres a tek around that involves tossing a cake into a blender w/sterile water and blending it for an instant LC. i know that was done "back in the day" with agar plates, the method is mentioned in TMC.

i'd just stick with "standard" LCs... they're easier, quicker, and less contam prone than any of those other ideas...


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Offlinefaceyneck
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Re: Liquid cultures and dunking [Re: creamcorn]
    #5962662 - 08/14/06 12:57 PM (17 years, 5 months ago)

Yes, however, I've had a lot of success by putting a chunk of mycelium into a jar of sterile water with a piece of glass, shaking the fuck out of it, and using that, however, I recently used a chunk from a cake that had been in the fruiting chamber for a while, and the jars I inoculated are taking a really long time to colonize. Usually, I get really good growth in about 48 hours.

...and now back onto the topic; thanks for clearing that issue up, CC.


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