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AndTheStoryEnds
Stranger
Registered: 08/02/06
Posts: 2
Last seen: 16 years, 10 months
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:( Second try and we gots nothin........
#5957446 - 08/12/06 07:47 PM (17 years, 5 months ago) |
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Alrighty, here we go. Thanks ahead to any who take the time to read this and offer some advice.
First try, my mixture was somewhat off. I followed a video that called for a near 3:1:1 mixture of brown rice flour/vermiculite/distilled water. The spores ordered were p azurescens in a print (only form they had available at the time). In short, the dry substrate proved insufficient to develop mycelium.
Second try, after doing more reading found a better mixture to be 2:1:1, so I followed that. In a very very clean room with a hepa filter, using a syringe purchased from sporeworks, I inoculated a new set of jars. They have been incubating at 86 for the last 4 or 5 days.
In short, Im worried because Im not seeing ANY mycelial growth around the inoculation points in the jars. I had read that sporeworks was a reliable place to purchase syringes. The strain was b+ (p cubes). The vermiculite is Hoffman, and the brown rice flour is hodgson mill brown rice flour.
any help would be much appreciated. Maybe Im just paranoid. Im not seeing contamination, but im not seeing anything else either.
Edited by AndTheStoryEnds (08/12/06 07:49 PM)
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Re: :( Second try and we gots nothin........ [Re: AndTheStoryEnds]
#5957591 - 08/12/06 09:01 PM (17 years, 5 months ago) |
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Must incubate longer, dont over heat it also, infact dont incubate at all just keep it in a dark place. Unless you have a tit incubator dont bother.
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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imtank
Self-Taught Fool


Registered: 07/05/06
Posts: 235
Loc: somewhere around Barstow,...
Last seen: 16 years, 2 months
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Re: :( Second try and we gots nothin........ [Re: mikeownow]
#5957716 - 08/12/06 09:51 PM (17 years, 5 months ago) |
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my grow of B+ took about a week and a half to show signs...and it took about a month+ to colonize...my stropharia,brazilian, and GT's are moving much quicker...maybe B+ is a slow colonizer
-------------------- 'To be positive at all times is to ignore all that is important, sacred or valuable. To be negative at all times is to be threatened by ridiculousness and instant discredibility.' -Kurt Kobain
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