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royer
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Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
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spore prints that are 4 years old???????
#5955631 - 08/12/06 03:35 AM (17 years, 5 months ago) |
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i have some spore prints that are 4 year old that i aquired. but i let them hydrate for a week and still nothing in the jars that i nocked up. is there a different way i should be going about this? maybe i should try it on my agar? thanks all
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 20 days, 17 hours
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Re: spore prints that are 4 years old??????? [Re: royer]
#5955648 - 08/12/06 03:41 AM (17 years, 5 months ago) |
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Give them some more time. As long there is no contamination, there is still chance they will germinate.
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saffr0n
Stranger

Registered: 07/15/06
Posts: 23
Last seen: 14 years, 6 months
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Re: spore prints that are 4 years old??????? [Re: Anno]
#5956517 - 08/12/06 01:13 PM (17 years, 5 months ago) |
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I just recently tried a 4 or more year old print I had with 4 WBS and 4 honey LC's and none of them did anything. I hope you have better luck, and let us know!
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: spore prints that are 4 years old??????? [Re: saffr0n]
#5956598 - 08/12/06 01:32 PM (17 years, 5 months ago) |
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With a sporeprint that is a few hours old, about one spore out of a hundred is viable. The other 99 are not going to germinate. Stretch that out to a one year old print, and less than one spore out of a thousand is viable, and it declines even more rapidly from there.
To play the numbers with a very old print, you'll want to do massive spore inoculation, but the problem is that the more mushroom spores you use, the more contaminant spores are going to be along for the ride, and they'll also be competing for the substrate.
Thus, fresh prints are always going to give the best results. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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thatiAM
Stranger

Registered: 06/14/06
Posts: 1,250
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Re: spore prints that are 4 years old??????? [Re: RogerRabbit]
#5956705 - 08/12/06 02:02 PM (17 years, 5 months ago) |
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I'd say try it at least. Use the agar to isolate the good myc, I guess. I recently used a roughly 2 year old print and it is working just fine.
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wiggles
Miffed a Milf


Registered: 11/09/05
Posts: 2,615
Last seen: 10 years, 5 months
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Re: spore prints that are 4 years old??????? [Re: thatiAM]
#5956802 - 08/12/06 02:58 PM (17 years, 5 months ago) |
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You should be fine. On top of normal germination time, you also have to add a few days for rehydration. You don't have to worry about the age so long as the prints were kept properly - there are members on here who've used spore prints that have been 30+ years old. Agar comes to mind.
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  You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye. Hunter S. Thompson
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