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Offlinenugjug
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Registered: 11/07/05
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Trying to figure out where the contamination came in
    #5953472 - 08/11/06 10:52 AM (17 years, 5 months ago)

I recently shot up 10 wbs/rye jars with multi-spore and all of them contaminated right where mycelium started to grow. I am assuming that this means it was a dirty syringe and not my inoculation technique. Does this assumption sound right?


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Invisiblemonstermitch
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Registered: 02/10/06
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Loc: Arizona Bay Flag
Re: Trying to figure out where the contamination came in [Re: nugjug]
    #5953482 - 08/11/06 10:57 AM (17 years, 5 months ago)

depends.

what was the contamination?


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Offlinenugjug
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Re: Trying to figure out where the contamination came in [Re: monstermitch]
    #5953494 - 08/11/06 11:04 AM (17 years, 5 months ago)

Woops. It was green mold. Or so I'm assuming. It was green.


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InvisibleTippinthru
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Registered: 04/07/05
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Re: Trying to figure out where the contamination came in [Re: nugjug]
    #5953543 - 08/11/06 11:20 AM (17 years, 5 months ago)

Assuming the jar content was sterile.

Contaminates can be from inside the syringe, or attach during the inoculation procedure. Or both.


Keeping an unsheathed needle wrapped in a alcohol saturated gauze pad, or paper towel at all times, during the inoculation procedure, is a good safety precaution, to preclude a needle getting funky..


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InvisibleCureCat
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Registered: 04/19/06
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Re: Trying to figure out where the contamination came in [Re: Tippinthru]
    #5955513 - 08/12/06 01:47 AM (17 years, 5 months ago)

Quote:

Tippinthru said:
Keeping an unsheathed needle wrapped in a alcohol saturated gauze pad, or paper towel at all times, during the inoculation procedure, is a good safety precaution, to preclude a needle getting funky..




Solid advice  :thumbup:


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Offlinenugjug
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Registered: 11/07/05
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Last seen: 5 years, 11 months
Re: Trying to figure out where the contamination came in [Re: Tippinthru]
    #5958013 - 08/12/06 11:51 PM (17 years, 5 months ago)

Quote:

Tippinthru said:

Keeping an unsheathed needle wrapped in a alcohol saturated gauze pad, or paper towel at all times, during the inoculation procedure, is a good safety precaution, to preclude a needle getting funky..




Yeah, that's what I did. The reason I was thinking that it was due to a dirty syringe and not due to lack of sterile jars is that the green mold hit exactly and only where the mycelium started to grow. That and the jars were PCed at 15psi for 2 and a half hours.


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OfflineRogerRabbitM
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Re: Trying to figure out where the contamination came in [Re: nugjug]
    #5958614 - 08/13/06 08:56 AM (17 years, 5 months ago)

Then you can be sure it was your inoculation process. Either dirty needle, print, or poor syringe making procedure.
RR


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