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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflineGreenScreen
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LC jar question
    #5952620 - 08/10/06 11:47 PM (17 years, 5 months ago)

how do most of you make your liquid culture jars so that you can shake them and it wont get whatever youre using for air exchange (tyvek, polyfil) wet and harbor contaminates?


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OfflineMacBluntski
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Re: LC jar question [Re: GreenScreen]
    #5952647 - 08/10/06 11:56 PM (17 years, 5 months ago)

get a marble and leave it in the jar when you cook it. stired not shaken...


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OfflineGreenScreen
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Re: LC jar question [Re: MacBluntski]
    #5952661 - 08/11/06 12:01 AM (17 years, 5 months ago)

well im just wondering. for thoes of you who do shake your lc's, how do you make your jars


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Invisiblemonstermitch
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Re: LC jar question [Re: GreenScreen]
    #5952688 - 08/11/06 12:11 AM (17 years, 5 months ago)

stirred, not shaken.

for shaken, check out TippinThru's set-up.

Or you can do a lid with two RTV ports.
One on each side.

pressure cook with lid loose.
allow to cool in pc for several (over night) hours.
open pc and tighten lids.
remove foil.

to inoculate and while colonizing:
use an empty syringe stuffed with poly-fil as an air port.
Sterilize the needle before you stick it in there.

don't leave a needle in any port for longer than five days.
switch between ports if you want constant gas exchange.

to shake:

remove air port and shake like hell.

leave an airport in one side and tilt the jar so you can aspirate through the other port with no vacuum.


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OfflineGreenScreen
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Re: LC jar question [Re: monstermitch]
    #5952719 - 08/11/06 12:20 AM (17 years, 5 months ago)

yea ive seen tippinthru's jars but i dont get how the liquid doesnt go through the filter disk like on this one

http://www.shroomery.org/forums/files/06-28/272921663-lid_3.jpg


Edited by GreenScreen (08/11/06 12:21 AM)


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: LC jar question [Re: GreenScreen]
    #5952831 - 08/11/06 01:04 AM (17 years, 5 months ago)

she just has all of the toys.

I use a magnetic stir plate and a teflon stir bar in my jars.

no need to shake.
it breaks up very nicely:



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