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OfflineKindnug
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Unsterile wild pans prints, how to use?
    #595211 - 04/01/02 05:58 AM (14 years, 8 months ago)

So I would like to use these wild prints I'm getting of copelandia cyenscens on agar plates; I was wondering the best way to do this? Is it possible by some freaky strange technique to sterilize the print to make syringes, or even to drop onto some PDA or BRF paste? Please help, I only am getting a couple prints!!! Thanks


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OfflineMacey Howard
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Post deleted by Moe Howard [Re: Kindnug]
    #595411 - 04/01/02 12:45 PM (14 years, 8 months ago)



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OfflineKindnug
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Re: Unsterile wild pans prints, how to use? [Re: Macey Howard]
    #595599 - 04/01/02 04:04 PM (14 years, 8 months ago)

Yeah unfortunately these are coming from AU in print form. Thanks for the tip though, I will order that bacteria resistent agar right now. Didn't know there was such a thing. Thanks!! Any other takers on this question? What if I made some very thin syringes to innoculate about 20 petris, will I get at least a half dozen or so sectors of good clean growth?


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InvisibleDreaMaTrix
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Re: Unsterile wild pans prints, how to use? [Re: Kindnug]
    #595631 - 04/01/02 04:45 PM (14 years, 8 months ago)

>What if I made some very thin syringes to innoculate about 20 petris, will I get at least a half dozen or so sectors of good clean growth?

Yes or no, who knows. You could just 'spread' the spores from the print straight onto the agar. If the print is contaminated it will show on the plate, next thing to do is isolate the mycelium away from the contam, onto new agar. You may have to do this a few times to get a 'clean' strain, depending on the contam.


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OfflineKindnug
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Re: Unsterile wild pans prints, how to use? [Re: DreaMaTrix]
    #595701 - 04/01/02 06:22 PM (14 years, 8 months ago)

Sweet; thanks. Just wanted to make sure that this was possible. As long as I can get a few sectors of good growth to trasfer, I should be set. Thanks.


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Re: Unsterile wild pans prints, how to use? [Re: Kindnug]
    #595739 - 04/01/02 07:24 PM (14 years, 8 months ago)

You may be suprised at the prints sterility. I have traded some azure prints that were printed from wild specimens. The contam rate was very low. When there was some contam the cultivator was able to isolate a clean strain from the plate.

Good luck,
Joshua


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InvisibleZen Peddler
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Re: Unsterile wild pans prints, how to use? [Re: DreaMaTrix]
    #596112 - 04/02/02 03:48 AM (14 years, 8 months ago)

In reality many prints out there even of domesticated cubensis may not be 100% sterile - wild prints are particularly bad at being contaminated - although i agree with Joshua in that ive received wild prints that were 100% sterile.
Youll need to streak (with an innoculation loop) some spores from one of those prints onto five petris or so of antibioticed agar. Then its a matter of sectoring or cutting off the areas of agar that are colonised without nasty molds, and placing these in new petris and then isolating rhizomorphic (or just linear with pan cyans) sector of mycelia and your ready to rumble.


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Re: Unsterile wild pans prints, how to use? [Re: Zen Peddler]
    #596126 - 04/02/02 04:02 AM (14 years, 8 months ago)

...but even still, you may never know what yer gonna get as far as fruiting & yeilds go when sectoring from multi-spore..
the best bet is cloning...


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Re: Unsterile wild pans prints, how to use? [Re: 40oz]
    #596131 - 04/02/02 04:06 AM (14 years, 8 months ago)

Unless your really unlucky - my first clone was from a large mazatapec fruit that i got from a casing - after i cloned it the casing yield was low. My clone is now cased and its performance has been the same as the original casing and not just that individual fruit. I cloned a fucked individual


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OfflineKindnug
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Re: Unsterile wild pans prints, how to use? [Re: Zen Peddler]
    #596175 - 04/02/02 06:12 AM (14 years, 8 months ago)

I have to do a lot of culture work before I can even consider taking a clone. Someone said that pan myc is more linear, is this true? Or is the best pan myco rhizo? I got the prints today, they are small and very sparse (he told me they would be); but there are 6 individual prints on 1 card. So I have to innoc with spores, but there should be plenty of substrain variety when taken as a whole. 1 question, can I use an exacto knife to streak spores or should I throw down and get an inoc loop? I have to buy that contam resistant agar still so hopefully they sell those too. I should have that by the end of the week so I'll be starting on this very soon. Kudos to Bluemeanie for this design

and here was the actual harvest

I will be so happy if I somehow can grow these over here in the US, they look delicious!!


Edited by Kindnug (04/02/02 06:15 AM)


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InvisibleZen Peddler
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Re: Unsterile wild pans prints, how to use? [Re: Kindnug]
    #597285 - 04/03/02 04:23 AM (14 years, 8 months ago)

i think pan cyan myc. is linear to cottony - or so it says in stamets


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Offlinezhukov
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Re: Unsterile wild pans prints, how to use? [Re: Kindnug]
    #597287 - 04/03/02 04:28 AM (14 years, 8 months ago)

Best of luck with the experiment, kindnug!
.....i'll be keeping my fingers crossed for ya :wink: 


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OfflineAnnoA
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Re: Unsterile wild pans prints, how to use? [Re: Kindnug]
    #597309 - 04/03/02 05:45 AM (14 years, 8 months ago)

>Or is the best pan myco rhizo?
Never.


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OfflineKindnug
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Re: Unsterile wild pans prints, how to use? [Re: Anno]
    #597615 - 04/03/02 02:26 PM (14 years, 8 months ago)

Thanks Bluemeanie and Anno, I knew I had read somewhere that pans do not really produce Rhizo growth. So what is best to sector and isolate then, tortmose or linear growth? Anything specific I should look for in the cultures? Thanks again everyone


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OfflineAzure
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Re: Unsterile wild pans prints, how to use? [Re: Kindnug]
    #597932 - 04/03/02 09:26 PM (14 years, 8 months ago)

Since pan mycelium doesn't differentiate into rhizomorphs, it's hard to say what's the best. I personally just isolate the thickest and fastest looking growth. Since the mycelium grows radially, whichever sector extends farthest from the center(where the spores germinated) tend to be the fastest. If that sector is also thick relative to the rest of the mycelium, then you have a winner.


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OfflineKindnug
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Re: Unsterile wild pans prints, how to use? [Re: Azure]
    #597940 - 04/03/02 09:32 PM (14 years, 8 months ago)

Sweet, that was perfect Azure. I think I am set, got my bacteria resistant agar on the way from Fungi Perfecti (man is he up there on some items!!!) as well as an inoc loop; I should be totally set now. Thanks again everyone!!


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