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Offlinefelixhigh
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help with paper chromatography - which paper and solvents?
    #5951479 - 08/10/06 06:19 PM (17 years, 5 months ago)

hello,
some days ago i caught myself having fun on making paper chromatography with various ethnobotanical samples (ok, i admit i did the pen/ink experiment too) and i got some pretty chromatograms on coffee filter (eck)with 92% alcohol.
with pure DCM (methylene chloride) as solvent i didn't get a clear separation of the compounds but with 3:2 alcohol (92%)/DCM it worked pretty fine. but i have some questions:

1) am i going to see all the components with the solvents i am using? i fear to miss compound spots due to a poor solvent choice.

2) what is the best kind of paper i can use? the guy at the chemical store asked me which kind and i didn't know what to answer so i decided to research more (i found nothing around the web on paper kinds)... so here is my research. :smile:
would common lab filter paper work well?

thanks for the attention,


FH:mushroom2:


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Offlinefelixhigh
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Re: help with paper chromatography - which paper and solvents? [Re: felixhigh]
    #5976262 - 08/18/06 03:52 PM (17 years, 5 months ago)

bump!


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Invisiblemoecat
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Registered: 02/19/05
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Re: help with paper chromatography - which paper and solvents? [Re: felixhigh]
    #5981660 - 08/20/06 03:11 PM (17 years, 5 months ago)

Skip using paper. Buy some silica gel TLC plates on Ebay.


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Offlinefelixhigh
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Re: help with paper chromatography - which paper and solvents? [Re: moecat]
    #5985386 - 08/21/06 11:37 PM (17 years, 5 months ago)

please give me a paper specification!
paper is easier and quicker and cheaper for me to obtain...


FH


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Offlinethe man
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Re: help with paper chromatography - which paper and solvents? [Re: felixhigh]
    #5985575 - 08/22/06 12:41 AM (17 years, 5 months ago)

hmm sounds interesting.. link to some more info??

peace felix


--------------------
And Moses Said "Let my mushrooms grow!"


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OfflinePsiloman
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Re: help with paper chromatography - which paper and solvents? [Re: felixhigh]
    #5986338 - 08/22/06 10:34 AM (17 years, 5 months ago)

I will try and help.Keep in mind that i will offer only basic information on the subject...

The wikipedia entry on chromatography : http://en.wikipedia.org/wiki/Paper_chromatography

I assume you know this already.

Here is a more practical guide although very limited on the paper :

http://www.sciencebuddies.org/mentoring/project_ideas/Chem_Chromatography_resources.shtml

100 sheets for 30 dollars? I bet you can afford it
http://www.sciencekit.com/category.asp_Q_c_E_431677 and the can become 200 if you cut them in half.I guess thats enough for starting off!

Now i didnt have time to present you with the "best of" this site but im sure your searching and sorting skills are fine so you can search this page : http://www.hplcweb.com/About_Chromatography/

From online books and guides to animations!

Also here is a nice handout : http://peer.tamu.edu/podium_poster_presentations/Paper%20Chromatography%20Handout.doc

Also search for Whatman chromatography papers and Schleicher & Schuellchromatography paper


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Offlinefelixhigh
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Re: help with paper chromatography - which paper and solvents? [Re: Psiloman]
    #5987038 - 08/22/06 04:08 PM (17 years, 5 months ago)

thank you psiloman, you have helped me a lot!


FH


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