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InvisibleStinkFist13
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colonization in 1 day?
    #5951211 - 08/10/06 05:07 PM (17 years, 5 months ago)

i put 5 cc of z strain spores in one 1/2 pint and it rapidly colonized half the jar in one day. i suspect bad spores. it is whiteish gray mycellum looking stuff. cow web mold maybe. i dont know


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2NO3- + 10e- + 12H+ → N2 + 6H2O
figure it out...i bet yall seen it once or twice


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Invisiblecreamcorn
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Re: colonization in 1 day? [Re: StinkFist13]
    #5951335 - 08/10/06 05:48 PM (17 years, 5 months ago)

well its definitely not mycelium.

how did you sterilize your jars?  its surprising that even a contaminate could spread that quickly, unless it was already under way before you inoculated.

sorry but juding from the fact you used 5cc in a single half pint and its contaminated, i suspect an inexperienced grower, not bad spores. :frown:


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Offlinewiggles
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Re: colonization in 1 day? [Re: creamcorn]
    #5951589 - 08/10/06 06:46 PM (17 years, 5 months ago)

I have to agree with creamcorn... 5cc is a lot to noc up a half pint jar. 1cc should be enough.

Did you PC them for a good solid hour at 15 PSI?


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You can turn your back on a person, but never turn your back on a drug, especially when its waving a razor sharp hunting knife in your eye.
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InvisibleStinkFist13
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Re: colonization in 1 day? [Re: wiggles]
    #5952413 - 08/10/06 11:05 PM (17 years, 5 months ago)

i suspected they were bad spores already judging by the fact they were clustered together by white stuff in the syringe. so i dumped it in there. no i havent got my pc yet but i boiled them for an hour and a 15 minutes, there brf.


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2NO3- + 10e- + 12H+ → N2 + 6H2O
figure it out...i bet yall seen it once or twice


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Invisibleint21h
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Re: colonization in 1 day? [Re: StinkFist13]
    #5953754 - 08/11/06 12:44 PM (17 years, 5 months ago)

It`s definately some kind of contam....nothing colonizes for one day...


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Invisiblecreamcorn
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Re: colonization in 1 day? [Re: int21h]
    #5954108 - 08/11/06 03:01 PM (17 years, 5 months ago)

the white stuff in the syringe was probably premature germination, caused by less than perfect syringe preparation.  its something to bring to a vendor's attention, because although spores are legal, once they germinate, they are not.  but it doesn't necessarily mean contamination.  if anything, it means they will grow even quicker once you've inoculated.

if we stop and consider you had germinated spores, and used so much solution, there's actually an off chance you do have some clean mycelium growing.  give it a few more days, if it thickens up and takes a more brilliant white appearance, your screwup combined with a screwed up syringe, might actually mean you did in fact get some quick colonization.  two wrongs could make a right here. :smile:

(is it nerdy of me or am i just showing my age that that i totally relate to int21h's nickname? :smile:)


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OfflineRogerRabbitM
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Re: colonization in 1 day? [Re: creamcorn]
    #5954196 - 08/11/06 03:41 PM (17 years, 5 months ago)

If you didn't PC, and it's colonized in one, or even three days, it's a contaminant, no ifs, ands or buts.

Even with a grain to grain transfer, it would take longer than that to colonize. It's mold, sorry.
RR


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Invisiblecreamcorn
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Re: colonization in 1 day? [Re: RogerRabbit]
    #5954329 - 08/11/06 04:23 PM (17 years, 5 months ago)

i beg to differ... i still say wait it out another few days and see.

RR - you've said yourself you've both never done a cake, and aren't a fan of LC.  well, spores that have germinated in a syringe, is basically an LC.  having personally shot as much as 5cc of LC in a half pint PF cake myself, i can tell you that that much liquid inoculate can cover 50% of the surface of a jar that small.  i can also promise you that with that much inoculate, you can see something happen in as little as 12 hours, as unbelivable as it sounds.  within 24 hours, it would appear to be very obvious greyish splotches covering a pretty large area.  a few days later, it thickens up and starts looking normal... so the first few days it won't expand outward much, but rather get denser where it is.

it sounds too good to be true, because in the long run it is, the extra moisture causes stalls towards the end of colonization, as moisture condenses in the jar, and sometimes gets sopped up by the uncolonized bits, making them very difficult to penetrate and causing the jar to take longer to finish in the long run.  so for anybody getting the light bulb over their heads that over-doing LC is a way to speed stuff up, think again.  you're in effect spreading a thin layer of freshly germinated live mycelium that's ready to go, all over the surface area of where you can see.  believe me, it doesnt take long to be able to observe growth.

ive seen it with my own eyes.  and even with the experience of seeing many colonized cakes, and seeing cobweb first hand, ill tell you its still hard to discern... but i promise i'm not making this stuff up and he very well might be ok.

the same can be said for a mold though, if it was germinated in the syringe, then you've got an LC of mold, which you spread around in mass quantity all over your substrate.  so certainly you'd see it take off quickly as well.

either way if he throws it out now without waiting a few days, we'll never know. :smile:  isolate it in case it is a contam so that it doesn't dirty up your growing area or jeopardize future projects, and let time tell us what happened...

ive no time this weekend, but if you'd like, i'll make a cake just for you on monday, inoculate it with an ungodly amount of good LC, and give you some day by day macro closeup shots so you can see it with your own eyes as well.  :thumbup:


Edited by creamcorn (08/11/06 04:35 PM)


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InvisibleCureCat
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Re: colonization in 1 day? [Re: creamcorn]
    #5955530 - 08/12/06 01:59 AM (17 years, 5 months ago)

Aaaaalrighty then.

My guess:

You had a germinated spore syringe.
You had an un-PC'ed substrate.
There was growth within 1 day.

To me this says that it was likely not the syringe, but the jar was contaminated with perhaps Cob web mold.  You said white/gray... That could be a thin layer of mycellium, but I'd say based on the info provided, it is cob web.

CreamCorn- I agree with you on everything, EXCEPT the likelihood of the occurrence.  I think that given the short time, it is hiiiiiighly unlikely that the substrate is colonized with mycellium.

I have seen germination in as short as 36hrs.  But I have never seen 50% colonization in 24hrs.

Lets hope he keeps the jar around a bit longer, and updates!  :smile:


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Invisibleint21h
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Re: colonization in 1 day? [Re: creamcorn]
    #5955862 - 08/12/06 08:12 AM (17 years, 5 months ago)

Quote:

creamcorn said:
the white stuff in the syringe was probably premature germination, caused by less than perfect syringe preparation.  its something to bring to a vendor's attention, because although spores are legal, once they germinate, they are not.  but it doesn't necessarily mean contamination.  if anything, it means they will grow even quicker once you've inoculated.

if we stop and consider you had germinated spores, and used so much solution, there's actually an off chance you do have some clean mycelium growing.  give it a few more days, if it thickens up and takes a more brilliant white appearance, your screwup combined with a screwed up syringe, might actually mean you did in fact get some quick colonization.  two wrongs could make a right here. :smile:

(is it nerdy of me or am i just showing my age that that i totally relate to int21h's nickname? :smile:)





No man, my nickname comes from the days I used to code in assembly...  :smile: :smile: :smile:


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Invisiblecreamcorn
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Re: colonization in 1 day? [Re: int21h]
    #5956019 - 08/12/06 10:24 AM (17 years, 5 months ago)

Quote:

int21h said:
No man, my nickname comes from the days I used to code in assembly...  :smile: :smile: :smile:




yeah thats what i was referring to. :wink:  used to do motorola 680x asm... but i know a famous dos interrupt when i see one :wink:


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InvisibleStinkFist13
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Re: colonization in 1 day? [Re: creamcorn]
    #5956778 - 08/12/06 02:37 PM (17 years, 5 months ago)

well i threw it out already,not saying this is creamcorns fault by any means but i asked others before creamcorn could reply to my message and they told me to toss it.so i tossed it. i still havesome 2 syringes and they all share the same kinda spores clumped by white stuff. so when i get my pc i will do it again.also i knocked up 4 1/2 pint lcs yesterday.so im really gonna watch my shit as it grows and see whats going on with all my spores.damm i should have waited on tossing that cake out, cus i only incubated for like 29 hours or somthing. only one thing to do TRY IT AGAIN!!!! and again and again...............


--------------------
2NO3- + 10e- + 12H+ → N2 + 6H2O
figure it out...i bet yall seen it once or twice


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InvisibleStinkFist13
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Registered: 05/30/06
Posts: 127
Re: colonization in 1 day? [Re: StinkFist13]
    #5956786 - 08/12/06 02:45 PM (17 years, 5 months ago)

try it creamcorn it may be too late for me to tell you what was really taking over my cake, but i certainly understand the concept you just explained. that is exactly what i was thinking before i knocked up that jar. im gonna go look in my trash can and see if the smashed cake is fruiting or somthing


--------------------
2NO3- + 10e- + 12H+ → N2 + 6H2O
figure it out...i bet yall seen it once or twice


Edited by StinkFist13 (08/12/06 02:46 PM)


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OfflineRogerRabbitM
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Re: colonization in 1 day? [Re: StinkFist13]
    #5956923 - 08/12/06 04:06 PM (17 years, 5 months ago)

It won't be. Even liquid or grains from a grain to grain transfer need 24 hours to recover from the concussion. Molds do not. Anything that colonized a cake in 24 hours, regardless of inoculation method or amount, is a mold. Period.
RR


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InvisibleStinkFist13
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Re: colonization in 1 day? [Re: RogerRabbit]
    #5957638 - 08/12/06 09:20 PM (17 years, 5 months ago)

i dug th cake out from the trash and it is fully colonized and bright white, it doesnt apper to be cob web.the texture is like a large large spit wad of paper or somthing it dont smell pleasant tho i think it might be a cantamed i dont know what ever it is it has colonized the cake whole just like mycellum and looks just like it also. it smells like rotting apples kinda or wine or somthing


--------------------
2NO3- + 10e- + 12H+ → N2 + 6H2O
figure it out...i bet yall seen it once or twice


Extras: Filter Print Post Top
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