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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Ok yeah i'm asking for some help.....
    #5944094 - 08/08/06 01:01 PM (17 years, 5 months ago)

OK i cooked up to much grain, couldn't find my bags so i had to use Jars. So i couldn't fit all the grain in one load. So then by the time i got to inoculating the jars from the first batch. The second batch i didn't get to autoclave started to spoil and ferment/mold over.

Now i would hate to let this grain go to waist. I don't mind chalking up a few jars in the name of science since they are already pretty much beat.

So my thoughts are that if i killed all the contam would it in theory acts like natural antibiotics for the substrate in some way? Would the mushrooms be OK growing on dead contam more so then just dead endospores? Is it a real waste of time?

Just some thoughts...... any ideas?


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Offlineshirley knott
not my real name
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Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 7 years, 28 days
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Re: Ok yeah i'm asking for some help..... [Re: Corporal Kielbasa]
    #5944376 - 08/08/06 02:52 PM (17 years, 5 months ago)

how come the second lot didn't get into the autoclave in time? was it :bongload: ?

you could re-sterilise the goo, but if it's already fermenting with something, you better give it an extra bit of time. trouble is, once there's loads of bugs in there, you're already fighting an uphill battle - sterilising only kills 99.999999999% of the bugs, and if you have 100000000000 bugs to start with, one may slip through.

give it a go, you have nothing to lose.


--------------------
buh


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Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: Ok yeah i'm asking for some help..... [Re: Corporal Kielbasa]
    #5944998 - 08/08/06 05:57 PM (17 years, 5 months ago)

Couldn't find your bags. It's maybe time to do a clean up?


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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Ok yeah i'm asking for some help..... [Re: micololo2]
    #5945004 - 08/08/06 05:59 PM (17 years, 5 months ago)

Nope just lost in space and time.  Was the last of them so maybe they got thrown out  :smile:


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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Ok yeah i'm asking for some help..... [Re: shirley knott]
    #5945014 - 08/08/06 06:03 PM (17 years, 5 months ago)

Yeah I will give it a go any ways.  Maybe twice. 
Oh and maybe that had a little to do with it :hehehe:


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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Ok yeah i'm asking for some help..... [Re: Corporal Kielbasa]
    #5953445 - 08/11/06 10:34 AM (17 years, 5 months ago)

and to answer the question........ no its the faulest smell ever cant even get near them to transfer grain.......


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: Ok yeah i'm asking for some help..... [Re: Corporal Kielbasa]
    #5964938 - 08/15/06 12:36 AM (17 years, 5 months ago)

It would be a waste of time if your plans include succes with those jars. But if you want to experiment a bit and see how your sterilization process works with no good results in mind, go ahead. Personally, I would toss the grain cause it´s cheap and readily available.
When moderate amounts of substrate/grain can´t fit inside my sterilizer, I cold storage the remaining stuff if it´s going to be used soon, if not I freeze it for prolonged storage and works good. I do this just with sawdust (not easy to find where I live) and organically grown grain (very expensive in my country).

Good luck.


--------------------
Sr_Setahongo


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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: Ok yeah i'm asking for some help..... [Re: Semilanceata]
    #5969028 - 08/16/06 10:10 AM (17 years, 5 months ago)

Good suggestions on the cold/freeze storage! I will try that next time.

Yeah it was just foul cooking those things. Never again.


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