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GreenScreen
bidibodi bidibu


Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
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innoculating lc while still hot
#5942666 - 08/07/06 11:34 PM (17 years, 5 months ago) |
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how much will it hurt if i accidentally inoculated a liquid culture while it was still kinda hot. it wasnt too hot, like it wouldnt burn me if i picked it up, but it was still pretty warm. think itl be ok?
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Yamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
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Re: innoculating lc while still hot [Re: GreenScreen]
#5942688 - 08/07/06 11:41 PM (17 years, 5 months ago) |
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ehh possibly, probably not though. Why wouldn't you just wait till it was cooled all the way?
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GreenScreen
bidibodi bidibu


Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
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Re: innoculating lc while still hot [Re: Yamidude]
#5942696 - 08/07/06 11:43 PM (17 years, 5 months ago) |
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haha. ummmm yea... i forgot
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: innoculating lc while still hot [Re: GreenScreen]
#5942734 - 08/07/06 11:53 PM (17 years, 5 months ago) |
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spores will survive pretty high temps, if you could hold it comfortably then you should be alright. maybe.
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: innoculating lc while still hot [Re: GreenScreen]
#5942740 - 08/07/06 11:55 PM (17 years, 5 months ago) |
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Start OVER, ASAP.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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GreenScreen
bidibodi bidibu


Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
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Re: innoculating lc while still hot [Re: Tippinthru]
#5942748 - 08/07/06 11:57 PM (17 years, 5 months ago) |
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ill see how it looks tomorow and if its not lookin ok then ill start another. its such a pain in the ass everytime to boil them. damn i really need to get a pressure cooker
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: innoculating lc while still hot [Re: GreenScreen]
#5942761 - 08/08/06 12:01 AM (17 years, 5 months ago) |
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problem with that is that it will take a few days before you will see growth from the spores. If you want to get your grow on the road, listen to Tip. or go get more jars and make new ones, along with old ones. then make 100 more, and then PC 300 mycobags and shoot those up.
LC is virtually free to make, if you have spores to spare, then spare away.
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GreenScreen
bidibodi bidibu


Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
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Re: innoculating lc while still hot [Re: Omnicracker]
#5942782 - 08/08/06 12:10 AM (17 years, 5 months ago) |
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actually i was starting this culture off of another liquid culture so it should grow a little quicker
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: innoculating lc while still hot [Re: GreenScreen]
#5942795 - 08/08/06 12:13 AM (17 years, 5 months ago) |
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Mycelium can take up to 120F for several minutes. I routinely pour hot agar over cultures in petri dishes when isolating away from bacteria. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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GreenScreen
bidibodi bidibu


Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
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Re: innoculating lc while still hot [Re: RogerRabbit]
#5942810 - 08/08/06 12:16 AM (17 years, 5 months ago) |
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yea i stuck it in the freezer for a couple minutes to cool it down right after i realized what i did so i think it might be fine. thanks for all the help. im still tryin to get things right but hopefully everything will be goin smooth soon
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