Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineGreenScreen
bidibodi bidibu
 User Gallery

Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
innoculating lc while still hot
    #5942666 - 08/07/06 11:34 PM (17 years, 5 months ago)

how much will it hurt if i accidentally inoculated a liquid culture while it was still kinda hot. it wasnt too hot, like it wouldnt burn me if i picked it up, but it was still pretty warm. think itl be ok?


--------------------


Extras: Filter Print Post Top
OfflineYamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: innoculating lc while still hot [Re: GreenScreen]
    #5942688 - 08/07/06 11:41 PM (17 years, 5 months ago)

ehh possibly, probably not though. Why wouldn't you just wait till it was cooled all the way?


Extras: Filter Print Post Top
OfflineGreenScreen
bidibodi bidibu
 User Gallery

Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
Re: innoculating lc while still hot [Re: Yamidude]
    #5942696 - 08/07/06 11:43 PM (17 years, 5 months ago)

haha. ummmm yea... i forgot


--------------------


Extras: Filter Print Post Top
InvisibleOmnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
Trusted Cultivator
Re: innoculating lc while still hot [Re: GreenScreen]
    #5942734 - 08/07/06 11:53 PM (17 years, 5 months ago)

spores will survive pretty high temps, if you could hold it comfortably then you should be alright. maybe.


--------------------




xxx..Learn Something..xxx


Extras: Filter Print Post Top
InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: innoculating lc while still hot [Re: GreenScreen]
    #5942740 - 08/07/06 11:55 PM (17 years, 5 months ago)

Start OVER, ASAP.


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


Extras: Filter Print Post Top
OfflineGreenScreen
bidibodi bidibu
 User Gallery

Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
Re: innoculating lc while still hot [Re: Tippinthru]
    #5942748 - 08/07/06 11:57 PM (17 years, 5 months ago)

ill see how it looks tomorow and if its not lookin ok then ill start another. its such a pain in the ass everytime to boil them. damn i really need to get a pressure cooker


--------------------


Extras: Filter Print Post Top
InvisibleOmnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
Trusted Cultivator
Re: innoculating lc while still hot [Re: GreenScreen]
    #5942761 - 08/08/06 12:01 AM (17 years, 5 months ago)

problem with that is that it will take a few days before you will see growth from the spores. If you want to get your grow on the road, listen to Tip. or go get more jars and make new ones, along with old ones. then make 100 more, and then PC 300 mycobags and shoot those up.

LC is virtually free to make, if you have spores to spare, then spare away.


--------------------




xxx..Learn Something..xxx


Extras: Filter Print Post Top
OfflineGreenScreen
bidibodi bidibu
 User Gallery

Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
Re: innoculating lc while still hot [Re: Omnicracker]
    #5942782 - 08/08/06 12:10 AM (17 years, 5 months ago)

actually i was starting this culture off of another liquid culture so it should grow a little quicker


--------------------


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: innoculating lc while still hot [Re: GreenScreen]
    #5942795 - 08/08/06 12:13 AM (17 years, 5 months ago)

Mycelium can take up to 120F for several minutes. I routinely pour hot agar over cultures in petri dishes when isolating away from bacteria.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineGreenScreen
bidibodi bidibu
 User Gallery

Registered: 07/02/06
Posts: 914
Loc: ation
Last seen: 5 years, 18 days
Re: innoculating lc while still hot [Re: RogerRabbit]
    #5942810 - 08/08/06 12:16 AM (17 years, 5 months ago)

yea i stuck it in the freezer for a couple minutes to cool it down right after i realized what i did so i think it might be fine. thanks for all the help. im still tryin to get things right but hopefully everything will be goin smooth soon


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* random innoculation gray1 892 3 05/03/01 04:10 PM
by gray1
* syringe volume vs. innoculant volume? augiemolly 406 1 05/25/03 10:18 PM
by PDU
* Corn Starch Liquid Mycelium Culture AlienPrimate 8,364 7 09/09/17 11:22 PM
by ComebackKid
* 24 Days too long for innoculation dr4g0n 590 2 12/18/02 04:40 AM
by daussaulit
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,284 38 05/11/01 11:08 AM
by MAIA
* liquid myclelium culture fizzacyst 1,630 8 06/03/03 09:17 PM
by lemunhed
* Innoculation procedure? Dexter666 4,443 15 03/04/10 04:22 AM
by bardleyrichard
* Innoculated at midnight... concerns of mine...
( 1 2 all )
Sko 2,722 22 05/10/03 08:52 AM
by Prisoner#1

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
880 topic views. 18 members, 155 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.009 seconds on 14 queries.