|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
ty1er4499
Stranger
Registered: 07/20/06
Posts: 14
Last seen: 13 years, 11 months
|
Time between sterilization and inoculation?
#5938498 - 08/06/06 09:35 PM (17 years, 5 months ago) |
|
|
Will waiting a couple weeks between sterilization and inoculation create any problems? My original plan was to give the jars some time to watch for any contams and to have them all ready to go when the syringes get here (im anxious!). My half pint jars have aluminun foil over their lids and now im worried that maybe they will dry out, since the aluminum doesnt keep them 100% airtight. It's not really a problem for me to redo the jars if i need to. What do you guys think?
|
ninjaturtle
Stranger
Registered: 07/17/06
Posts: 22
Last seen: 10 years, 4 months
|
Re: Time between sterilization and inoculation? [Re: ty1er4499]
#5938622 - 08/06/06 10:06 PM (17 years, 5 months ago) |
|
|
a couple of weeks is a long time.
|
Lily_Morgan
I'm #1 !!

Registered: 07/05/06
Posts: 437
Loc: Eastern Shore
Last seen: 16 years, 3 months
|
Re: Time between sterilization and inoculation? [Re: ninjaturtle]
#5938630 - 08/06/06 10:07 PM (17 years, 5 months ago) |
|
|
I doubt any contams will show up in the jars before you actually inoculate.
|
Capless
Student


Registered: 06/25/06
Posts: 365
Last seen: 14 years, 10 months
|
Re: Time between sterilization and inoculation? [Re: Lily_Morgan]
#5938635 - 08/06/06 10:09 PM (17 years, 5 months ago) |
|
|
If its properly sterilized no contams should come about.
You may leave jars out for long periods of time to dry out, then inoculate them with massive amounts of LC for super fast growth.
-------------------- http://www.toolband.com/ "... This body holding me reminds me of my own mortality. Embrace this moment. Remember, We are eternal. all this pain is an illusion...."
|
RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
|
Re: Time between sterilization and inoculation? [Re: Capless]
#5939233 - 08/06/06 11:42 PM (17 years, 5 months ago) |
|
|
Your jars should be inoculated as soon as they cool down. 'Sterilization' is a relative term in this business and never complete. Make up a fresh batch of jars when you get your spores. Jars already made will let you test your procedure, and if they go green or get smelly, you know next time to do things differently. However, don't inoculate them. For best results, always use fresh substrate. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
Edgekrusher
God
Registered: 10/10/05
Posts: 674
Last seen: 17 years, 1 month
|
Re: Time between sterilization and inoculation? [Re: RogerRabbit]
#5939360 - 08/07/06 12:08 AM (17 years, 5 months ago) |
|
|
4-6 hours is all I allow
|
Velocity92C
Stranger
Registered: 07/22/06
Posts: 170
Last seen: 14 years, 9 months
|
Re: Time between sterilization and inoculation? [Re: Edgekrusher]
#5939478 - 08/07/06 12:45 AM (17 years, 5 months ago) |
|
|
I'm a rookie but I have a little experience with this. I ordered 6 premade jars from some company about 3 weeks ago. I just inoculated them 3 days ago and threw them in the incubator. I sterilized a nail, poked 4 holes in each, sterilized some foil with alcohol and covered each with alcohol for about 2 hours before I inoculated them. 3/6 jars show mycelium growth today, only 3 days after inoculation (with spores).
On the other hand, I made up 10 jars to sterilize today, with holes already on the lids and foil covering the lids. I opened up the cabinet they were sitting in today and one jar was 50% covered with what almost looked like mycelium even though they had no spores anywhere near them. A quick whiff of it quickly confirmed that it was contamed. No inoc, sealed up pretty good and already contamed.
The jars that were sent to me were sealed airtight however, because you could hear the air escaping as I punched the initial hole in each one. The ones in the cabinet were just sealed with aluminum covering the 4 holes on the lids. Still weird though, as the ingredients were baked ahead of time.
Anyway, it's your call. If your jars were sealed tight after sterilization then in my opinion you're fine. Contams are tricky though, already ran into 2 and I haven't even finished my first grow yet.
|
Capless
Student


Registered: 06/25/06
Posts: 365
Last seen: 14 years, 10 months
|
Re: Time between sterilization and inoculation? [Re: Velocity92C]
#5939493 - 08/07/06 12:50 AM (17 years, 5 months ago) |
|
|
Use tyvek. I made your the same mistake as you with my very first attempt at growing. IDK if we can link to the USPS website, but I can get you a link to free envelopes. Tyvek creates a nice seal when PCing to keep contams out.
-------------------- http://www.toolband.com/ "... This body holding me reminds me of my own mortality. Embrace this moment. Remember, We are eternal. all this pain is an illusion...."
|
Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
|
Re: Time between sterilization and inoculation? [Re: ty1er4499]
#5939513 - 08/07/06 12:56 AM (17 years, 5 months ago) |
|
|
If possible, you should inoculate PC'ed jars, ASAP, after the internal jar content has cooled to equal an average household room temperature where the jars are stored.
If you REFRIGERATE the INOCULATE, you should also take them out, and allow their temperature to equalize to room temperature, also.
Generally, if the content of both are in the area of 70, to 78F.
They are good to go.
Grain spawn, PF jars have a gas exchange provision. Rh of the jar storage area in the average household, is usually far lower that inside the jars. Consequently, the Rh of the two will attempt to become equal, through the gas exchange filter.
Which causes considerable moisture loss, from inside the jars.
Over time, that moisture loss can throw the jar content far outside the optimal range, it was 10, or 12 hours after PC'ing.
I would wager, you don't open canned food, then place that open can in an closet, shelf, filing cabinet, or whatever for 2 weeks, before you eat the content. Because eating it, would most probably expose you to mild, to severe food poisoning.
Certainly, well prepared grain spawn, and PF jars have more protection than an open can of moist food, stored unrefrigerated.
If you want a visual example of what is possible, sanitize an unopened metal can of soup, spaggetios, baked beans, or whatever is on hand. Also sanitize a sharp instrument, that you can cleanly pierce the metal can with. Have a piece or 2 of alcohol saturated micropore tape, next to the can. Taking all the sanitary precautions possible, pierce the can, and instantly cover the hole with micropore tape..
Then, mark the can with something big, bold, bright, and vivid (POISON). So that you, or anyone cannot mistake that can for any other, and place that can somewhere at normal room temperatures, for 2 weeks.
Then, inspect that can. It is highly probable, some weird nasty bubbling stuff from inside the can, is trying to push itself through the micropre tape. If you care to, open the can, and visually inspect the content. What you see, will not be appetizing.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
|
tonyperez420
Shaman Rasta

Registered: 11/03/04
Posts: 1,234
Last seen: 10 years, 11 months
|
Re: Time between sterilization and inoculation? [Re: ty1er4499]
#5939793 - 08/07/06 03:01 AM (17 years, 5 months ago) |
|
|
Quote:
ty1er4499 said: Will waiting a couple weeks between sterilization and inoculation create any problems? My original plan was to give the jars some time to watch for any contams and to have them all ready to go when the syringes get here (im anxious!). My half pint jars have aluminun foil over their lids and now im worried that maybe they will dry out, since the aluminum doesnt keep them 100% airtight. It's not really a problem for me to redo the jars if i need to. What do you guys think?
dude, I left jars out on top of my frige in my kitchen for up to a month, and there were days when it was 100 +++ in my kitchen
ralphstersspores + month old jars = nothing but pearly white spawn
it was of course tightly wrapped with alum foil on top
|
ty1er4499
Stranger
Registered: 07/20/06
Posts: 14
Last seen: 13 years, 11 months
|
Re: Time between sterilization and inoculation? [Re: tonyperez420]
#5940415 - 08/07/06 10:58 AM (17 years, 5 months ago) |
|
|
Thx for your responses! Ive decided that im going to buy another set of jars and make up some fresh ones when my spores get here. Ill directly inoculate the new jars with a syringe, however im going to make an LC and use that to knock up the jars i already have.
-------------------- Play Plasma Pong!
|
|