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Offlinetommythehort
will eat yourbeef slow
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Registered: 07/04/06
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Loc: Christmas
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Recycling PF jars that never germinated?
    #5936644 - 08/06/06 11:07 AM (17 years, 7 months ago)

I have about 15 PF jars that never germinated, never got contammed.... nothing. Other jars from this batch are doing great, some I had to slow down in fact because my poop isn't here yet. Would it be a terrible idea to throw these jars back in the pc for 60-90 mins or so and re-inoculate them from a liquid culture?

http://www.geocities.com/ghettoyhetti2000/p8050011.jpg

^That is my setup... everything in there is incubating. The white box is an air purifier/ionizer running at the lowest setting. The top jars are 1 pt. B+, the 2nd tier is millet & brf+fine vermiculite both hawaiian, next one is 2 jars of LC hawaiian and a 1qt WBS Puerto Rican and a 1 qt wheat berry hawaiian, below is a bunch of puerto rican/hawaiian.... the group to the left has not shown any sign of life in 2 weeks.

Throwing them in in a few minutes

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OfflineCantiSama

Registered: 10/21/04
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936665 - 08/06/06 11:18 AM (17 years, 7 months ago)

dude, put those things in the dark. when you leave them in the light like that it will cause them to pin in the jars and that isn't what you want. you don't need an air purifier in the incubator, either. as for reusing jars, i say don't do it. the water content may be off since you inoculated them before and it really isn't that hard to just make new substrate.


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Tiger got to sleep, bird got to land;
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InvisibleFooMan
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936670 - 08/06/06 11:19 AM (17 years, 7 months ago)

If they didn't germinate the first time, there may have been something wrong. Possibly the moisture content.


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Quick WBS Prep

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Offline2FiNiTe
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Registered: 06/12/06
Posts: 1,635
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936671 - 08/06/06 11:19 AM (17 years, 7 months ago)

As long as they didnt show signs of contamination you should be ok.  You might want to resoak/boil it just to be sure your still holding the right moisture level.  But that is depending on how much time they sat while you were waiting on germination.

I recycle birdseed all the time.  It about the only thing I work with.  I was going to try WBS and BRF and I see you've already got that going.  You should let us know how that goes, I'd be interested to see.  Mine should be starting soon but I'm in the middle of moving 200 miles away so I have a little down time in which im spending colonizing about 20 qt. jars with wbs. I'll use all the gigantor storage bins and rubbermade containers I bought for storage during the move.  Ahhh I cant wait.  :smirk:


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InvisibleFooMan
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Re: Recycling PF jars that never germinated? [Re: 2FiNiTe]
    #5936674 - 08/06/06 11:23 AM (17 years, 7 months ago)

Quote:

2FiNiTe said:
As long as they didnt show signs of contamination you should be ok.  You might want to resoak/boil it just to be sure your still holding the right moisture level.  But that is depending on how much time they sat while you were waiting on germination.

I recycle birdseed all the time.  It about the only thing I work with.  I was going to try WBS and BRF and I see you've already got that going.  You should let us know how that goes, I'd be interested to see.  Mine should be starting soon but I'm in the middle of moving 200 miles away so I have a little down time in which im spending colonizing about 20 qt. jars with wbs. I'll use all the gigantor storage bins and rubbermade containers I bought for storage during the move.  Ahhh I cant wait.  :smirk:




He's talking about PF cakes. How can he guess how much moisture is in them? Sure, WBS can be rehydrated, but verm/BRF is much different. I say either wait it out to see if they're late bloomers or toss the cakes and start over.


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InvisibleFooMan
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936685 - 08/06/06 11:27 AM (17 years, 7 months ago)

Screw it, if you have enough LC going already and you don't care about possibly wasting it, go for it! It may just work. That's the good thing about LC's.


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Offlinetommythehort
will eat yourbeef slow
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Registered: 07/04/06
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936686 - 08/06/06 11:27 AM (17 years, 7 months ago)

They are in the dark, obviously I only turned the lights on to take the picture. They are also at a constant 83 degrees and 60% humidity just in case you were wondering.

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InvisibleFooMan
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936698 - 08/06/06 11:33 AM (17 years, 7 months ago)

Light or dark won't hurt anything. If the cakes pin invitro just birth them. No big deal.

What concerns me is quote under your name:

"will eat your beef slow"

WTF does that mean? I mean, if you were a chick I'd be somewhat aroused, but it doesn't look like you are. Basically I'm hoping that's some kind of a joke and not a reference to love of man meat! :crazy:

Help me understand :confused:


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Offlinetommythehort
will eat yourbeef slow
Male

Registered: 07/04/06
Posts: 75
Loc: Christmas
Last seen: 16 years, 5 months
Re: Recycling PF jars that never germinated? [Re: FooMan]
    #5936715 - 08/06/06 11:43 AM (17 years, 7 months ago)

Lol nobody has ever taken it that way before... it is a quote from this retarded "D12" song. One of those guys says in some song "I'll eat your beef slow" referencing beef as like a conflict between he and another... I just always thought it was ridiculous, that's why I used it.

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Offlinetommythehort
will eat yourbeef slow
Male

Registered: 07/04/06
Posts: 75
Loc: Christmas
Last seen: 16 years, 5 months
Re: Recycling PF jars that never germinated? [Re: FooMan]
    #5936721 - 08/06/06 11:46 AM (17 years, 7 months ago)

I am going to give it a shot, the only thing that worries me, at each point of inoculation there is a wet spot.... been there since inoculation, i took the tops off and sniffed and it just smells like brf... I'm not too worried about bacteria since it has looked like that for so long but if it is, will the PC take care of it?

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InvisibleFooMan
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936744 - 08/06/06 11:54 AM (17 years, 7 months ago)

Quote:

tommythehort said:
I am going to give it a shot, the only thing that worries me, at each point of inoculation there is a wet spot.... been there since inoculation, i took the tops off and sniffed and it just smells like brf... I'm not too worried about bacteria since it has looked like that for so long but if it is, will the PC take care of it?




I would just start over. I didn't know you had opened the jars already. PC'ing them again will just cause them to lose more moisture. you can try, but it may not be worth the time.


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InvisibleFooMan
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Re: Recycling PF jars that never germinated? [Re: tommythehort]
    #5936756 - 08/06/06 11:58 AM (17 years, 7 months ago)

Quote:

tommythehort said:
Lol nobody has ever taken it that way before... it is a quote from this retarded "D12" song. One of those guys says in some song "I'll eat your beef slow" referencing beef as like a conflict between he and another... I just always thought it was ridiculous, that's why I used it.




Alright then! Keep eating your beef! :grin:


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OfflineRogerRabbitM
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Re: Recycling PF jars that never germinated? [Re: FooMan]
    #5936816 - 08/06/06 12:14 PM (17 years, 7 months ago)

Don't recycle jars. 'Sterilization' is relative and never complete. It gives us a 'window of opportunity' for our mycelium to capture the substrate. Toss out the contents of old jars and make up fresh ones for each batch.

Dark is overrated. Normal room lighting has little to no effect on colonizing mycelium. Light only becomes a pinning trigger when it's in conjunction with full colonization and an increase in air exchange with a corresponding decrease in CO2 levels. I've allowed jars to colonize on an open shelf at normal indoor room temperature and normal indoor room lighting for years with no adverse results.
RR


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