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Hotsauce72
ShapeShifter


Registered: 07/29/06
Posts: 58
Last seen: 15 years, 10 months
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Tight or loose?
#5935132 - 08/05/06 05:43 PM (17 years, 5 months ago) |
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Long time reader first time poster. I'm incubating wbs in pint jars with a layer of tyvek under the lid and polyfil in the inoculation point of the lid. The lids are screwed on firmly. I was wondering if there is enough gas exchange. Thanks for your help.
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The_Chariot
Stranger


Registered: 07/10/06
Posts: 127
Last seen: 13 years, 7 months
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Re: Tight or loose? [Re: Hotsauce72]
#5935139 - 08/05/06 05:49 PM (17 years, 5 months ago) |
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Yeah should have no promblem with that. Just make sure to Wrap the jar with a coffe filter and aluminum foil, after you inculate. It helps keep more contamination out.
Check out www.fungifun.org/English/Pftek
Thats would I do to a "T" with some minor additions.
-------------------- My will be ashes and a casket for all I want. The apocalypse, this way comes. So come. Sing the song. Repent kingdom, the end is here and it won't remain silent. The devil is in Atlanta. The army is surrounded. The civilians panic.
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Edgekrusher
God
Registered: 10/10/05
Posts: 674
Last seen: 17 years, 1 month
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if it ain't tight, it ain't right.
That's my motto on things good.
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Hotsauce72
ShapeShifter


Registered: 07/29/06
Posts: 58
Last seen: 15 years, 10 months
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Thanks edge and chariot!
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Quote:
The_Chariot said: Yeah should have no promblem with that. Just make sure to Wrap the jar with a coffe filter and aluminum foil
A coffee filter won't do diddily for filtering and the foil may block gas exchange. A coffee filter is used in place of aluminum foil during PC'ing. The reason for this is that it will catch drops of water in the PC and keep them off of the lid. It also easily evaporates the moisture once remove from the PC unlike foil that traps it to keep the filter underneath dry. It IS NOT a sufficient filter for mycological work.
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Quick WBS Prep
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The_Chariot
Stranger


Registered: 07/10/06
Posts: 127
Last seen: 13 years, 7 months
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Re: Tight or loose? [Re: FooMan]
#5936950 - 08/06/06 12:55 PM (17 years, 5 months ago) |
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ah i c...I just used it for safe measures. Really never had a promblem with it.
-------------------- My will be ashes and a casket for all I want. The apocalypse, this way comes. So come. Sing the song. Repent kingdom, the end is here and it won't remain silent. The devil is in Atlanta. The army is surrounded. The civilians panic.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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I'm not saying not to use it, just not to count on it as an actual filter. You have to be very clear with most people or next thing you know, you'll have people using coffee filters in place of Tyvek and wonder why they have contams.
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Quick WBS Prep
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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many people use the poly-fil without the tyvek.
many people use the tyvek without the poly-fil.
you should be fine.
however, having the tyvek under the lid means it could get wet during shaking.
the moisture could wick to the poly-fil. contams could than wick into your jars. bad news.
I now put the tyvek over the lid and make very small holes in the lid. Enough for gas exchange, but not to allow grain through the lid to get to the tyvek.
no wet filter material means no wicking of contams in.
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jmg5
deadicated


Registered: 11/23/05
Posts: 635
Loc: miles above you
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Quote:
monstermitch said: I now put the tyvek over the lid and make very small holes in the lid. Enough for gas exchange, but not to allow grain through the lid to get to the tyvek.
no wet filter material means no wicking of contams in.
This is the right way to do it. I take a hammer and a nail and put two small holes in the lid and put the tyvek over it. I push a needle through one of the holes and seal the jar, then I pull the needle out and put a peice of tape over it, now you can see a little hole and know exactly where to inject.
The filter stays bone dry when it's above the lid, this justs eliminates another source of contamination.
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76degrees
Apprentice

Registered: 06/08/06
Posts: 350
Loc: Middle Earth
Last seen: 17 years, 4 months
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Re: Tight or loose? [Re: jmg5]
#5937111 - 08/06/06 01:56 PM (17 years, 5 months ago) |
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whoops... I put the tyvek under the lid. Damn. I'll know more in a week. We'll see what happens.
-------------------- The world is changed. I feel it in the water. I feel it in the earth. I smell it in the air. Much that once was is lost, for none now live who remember it.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Tight or loose? [Re: 76degrees]
#5937152 - 08/06/06 02:12 PM (17 years, 5 months ago) |
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Quote:
76degrees said: whoops... I put the tyvek under the lid. Damn. I'll know more in a week. We'll see what happens.
Don't sweat it. Although I wouldn't rule it out as a possibility, I've never experience the contam wicking phenomenon myself.
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Quick WBS Prep
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Tight or loose? [Re: 76degrees]
#5937161 - 08/06/06 02:16 PM (17 years, 5 months ago) |
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Quote:
76degrees said: I put the tyvek under the lid.
it shouldnt be a problem, thats what I always did
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: Tight or loose? [Re: Prisoner#1]
#5937798 - 08/06/06 05:45 PM (17 years, 5 months ago) |
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Quote:
Prisoner#1 said:
Quote:
76degrees said: I put the tyvek under the lid.
it shouldnt be a problem, thats what I always did
Here as well it helps keep metal lids from rusting as quick also.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Tight or loose? [Re: hyphae]
#5937817 - 08/06/06 05:52 PM (17 years, 5 months ago) |
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and when they do, it doesnt stain the jars
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