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North Spore Shop: Cultivation Supplies

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Offlinejfoster
"That" guy

Registered: 05/14/06
Posts: 294
Last seen: 7 years, 10 months
Liquid culture
    #5934308 - 08/05/06 11:41 AM (17 years, 5 months ago)

when making a liquid culture can you use tap water or does it have to be distilled? also, when you inoculate mycobags with lc do you break up the bag afterwards?


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Invisibleitsbreck
Crunk
Male
Registered: 01/25/06
Posts: 447
Re: Liquid culture [Re: jfoster]
    #5934343 - 08/05/06 11:52 AM (17 years, 5 months ago)

i use tapwater all the time... distilled is better though, a gallon is like a buck and a half. Never used mycobags...


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OfflineYamidude
Stranger

Registered: 06/15/06
Posts: 957
Last seen: 15 years, 5 months
Re: Liquid culture [Re: itsbreck]
    #5934359 - 08/05/06 11:59 AM (17 years, 5 months ago)

i gently break up my home made mycobags then wait till about 30-40 percent them break up again.


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Liquid culture [Re: jfoster]
    #5934406 - 08/05/06 12:21 PM (17 years, 5 months ago)

Tap water works. I would suggest boiling it, and add whatever you are using as nutrient, while boiling. Then, filter through 4 or 5 stacked coffee filters. Then pour in a jar, and PC 15 minutes, at 15 psi.

If you are the impatient type.

I would suggest inoculation of a filter patch bag, all on top the content, and/or down the sides. That way, you "should" see visible germination ASAP, and very visible.

Once that colonization is around 10%, knead the bag, to spread the colonized content around in it. Then, let it alone until 100%.

Filter patch bags, should Not he kneaded, or handled to much.
Doing so, impedes growth. To much, and it will stall.


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Perfection is attained by slow degrees; it requires the hand of time...
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North Spore Shop: Cultivation Supplies


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