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rammbler
El primo de losmatador


Registered: 05/17/05
Posts: 82
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strain isolation the hardway
#5932802 - 08/04/06 09:18 PM (17 years, 5 months ago) |
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I started out with an amazon print that a friend made by a friend in not the most sterile conditions. so i made some MEA in 4 1/2 pint jars that were poured at 25ml and left to cool for a day before I did a multispore inoculation in each under a flowhood of which all but one became contamenated and even the one had a spot of somekind of black mold so then I made 6 more MEA jars, which I poured at 50ml this time because the first jars were very thin in the middle, in hopes of taking the best parts out and getting a clean strain out of all this mess. so I then I would cut out a square about 3/8s and placed upside down on the new jars. one at a time of coarse. I did 4 jars like this 2 of which I dipped in h2o2 and 2 of them I just did from jar to jar. and this is what I came out with Now the first jar there looks like it has something surrounding it, any takers on what it is? and thats one that got dipped.all the rest came out just fine. the other 2 jars I did another multispore inoculation with another amazon print the was made by the same person in the same conditions. I did one a streak and one I just touched it inthe center the one that i streaked has the same kind of stuff starting to surround it as well. I would like to thank all the nice folks on here that share thier knowledge and some dumbluck with all of us novice. Roadkill, agar, Hippechick have all really inspired me. thanks guys, and girls.
-------------------- "Fuck'em if they can't take a joke" BOB
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em_bre_O
shroomery'sEmbryoticAsshole


Registered: 05/27/02
Posts: 2,312
Loc: In the stages of develope...
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Re: strain isolation the hardway [Re: rammbler]
#5934998 - 08/05/06 04:06 PM (17 years, 5 months ago) |
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J.R. Bob Dobbs............the power of slack!
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rammbler
El primo de losmatador


Registered: 05/17/05
Posts: 82
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Re: strain isolation the hardway [Re: em_bre_O]
#5935067 - 08/05/06 04:51 PM (17 years, 5 months ago) |
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PRAZEBOB
-------------------- "Fuck'em if they can't take a joke" BOB
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: strain isolation the hardway [Re: rammbler]
#5936315 - 08/06/06 06:53 AM (17 years, 5 months ago) |
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ever thought about using petri dishes?
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