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Offlinecorey420
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Registered: 12/20/05
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contam. problems?
    #5932024 - 08/04/06 04:19 PM (17 years, 5 months ago)

hi my friend has made 100 pf jars and for the past 3 runs about 20-30 of them get contamed, and its usally black. we pressuse cook for an hour, let cool for 12hr then inoculate. is it ok to whipe needle with alcohol after heating it then inoculate?thanks all


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Offlineimtank
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Re: contam. problems? [Re: corey420]
    #5932038 - 08/04/06 04:24 PM (17 years, 5 months ago)

yea thats fine...there must be something else your doing wrong in your sterilization technique


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Invisiblemonstermitch
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Re: contam. problems? [Re: corey420]
    #5932166 - 08/04/06 05:11 PM (17 years, 5 months ago)

molds are easily killed in the pressure cooking process.

therefore your mold is coming from a mistake afterwards.

probably inoculation.

yes, you can wrap the needle in an alcohol soaked paper towel or whatever after heating.

RR suggests inoculating with the needle still hot and allowing the
first few drops of solution to cool the needle.

So I guess that would be flame sterilization for every hole.
With this way I would only use two holes.

agar has a great thread about open air inoculation

where the needle never touches open air and is always wrapped in alcohol.

If you improve your inoculation method, your numbers should improve greatly.


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OfflineRogerRabbitM
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Re: contam. problems? [Re: monstermitch]
    #5932192 - 08/04/06 05:23 PM (17 years, 5 months ago)

Even a simple pasteurization will kill molds, so if you're pressure cooking your jars, definitely examine your inoculation procedures.

One other thing. If you make your mix too wet, it causes the vermiculite barrier to become damp. A damp verm layer will allow the molds to spread from the outside air in. If the mold starts at the top of your jar, that's what is happening. If it starts from the inoculation sites, fix that procedure.
RR


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Offlinecorey420
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Last seen: 10 years, 8 months
Re: contam. problems? [Re: RogerRabbit]
    #5934287 - 08/05/06 11:31 AM (17 years, 5 months ago)

k thanks guys


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