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TrippinTeddy
Lost Voyager


Registered: 06/07/04
Posts: 18,461
Loc: Returning Video Tapes
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WBS Confusion
#5930227 - 08/04/06 12:48 AM (17 years, 5 months ago) |
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What is the best way to prepare wbs for inoculation? I think I'm confused on this subject. From the way I have read it and understand it you should soak for 24 hours then simmer for 30 the strain and pressure cook?
I have also heard that simmering is not necessary if you soak for 24 hours.
I have tried twice now and failed both times.
This time around I'm thinking of simmering for 30 minutes prior to soaking. After the simmer I will soak for for 24 hours.
Any of you that are experience with wbs please help!
-------------------- ToiletDuk said: For the record, I would show you my butthole but you would fall down and worship it as a God and you would give up everything to roam the land converting the heathens by fire and sword. Millions would die. No, no the cost is too great. I cannot.
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mikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
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Well I use rye and what I do is soak for 24 and simmer for 5 and then after simmer roll them around on a towel and load into jars then take some tyvek cover then put lid and wring on works like a charm then I pc for 55 minutes and then COol over night then i inoculate and I do it in open air all the time with success and my house has anything but still air
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.
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Capless
Student


Registered: 06/25/06
Posts: 365
Last seen: 14 years, 10 months
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It's really up in the air. Pretty much whoever you talk to will give you a different story each time.
A respectable member gave me this advice.
1) Using a close-knit strainer rinse birdseed in warm water till the water runs clear. Then dump it into soaking vessel.
2) Soak for 12-24 hours in warm water, a cup of coffee, and a teaspoon of gypsum. You may soak it for +24 hours, but change out the solution if you do that. If your mixture contains sunflower seeds strain them off the top. Stir after you've strained the first layer, more will arise.
3) You'll know when the soaking is complete when the water gets dirty and it smells bad. That's your cue. Time to rinse again in warm water. This time rise till the water runs clear again and let the WBS drain out for 5-10 minutes each bit. Be sure to tilt the strainer side to side to make sure all the water gets out. Put in another pot for 'holding.'
4) Load up your jars/bags.
-------------------- http://www.toolband.com/ "... This body holding me reminds me of my own mortality. Embrace this moment. Remember, We are eternal. all this pain is an illusion...."
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Spacerace
Stranger
Registered: 08/03/06
Posts: 16
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I think you could simmer after the soak instead of before to get maximum moisture content in the seeds or whatever. Whenever I simmer I let the steam help strain it because thats an important step. Alot really evaporates from it. Before my jars wouldnt work when I would strain without the steam. This insured the step for me.
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TrippinTeddy
Lost Voyager


Registered: 06/07/04
Posts: 18,461
Loc: Returning Video Tapes
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Re: WBS Confusion [Re: mikeownow]
#5930260 - 08/04/06 12:58 AM (17 years, 5 months ago) |
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Quote:
mikeownow said: Well I use rye and what I do is soak for 24 and simmer for 5 and then after simmer roll them around on a towel and load into jars
Whats the purpose of rolling in the towel?
-------------------- ToiletDuk said: For the record, I would show you my butthole but you would fall down and worship it as a God and you would give up everything to roam the land converting the heathens by fire and sword. Millions would die. No, no the cost is too great. I cannot.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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A long soak isn't neccessary. I don't care who says it is. Check out the WBS link in my sig, then check out the results in my gallery.
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Quick WBS Prep
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Quote:
TrippinTeddy said:
Quote:
mikeownow said: Well I use rye and what I do is soak for 24 and simmer for 5 and then after simmer roll them around on a towel and load into jars
Whats the purpose of rolling in the towel?
It gets rid of the excess water.
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Quick WBS Prep
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Sinthetic
Stranger


Registered: 12/05/05
Posts: 812
Last seen: 15 years, 6 months
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Re: WBS Confusion [Re: FooMan]
#5930599 - 08/04/06 06:28 AM (17 years, 5 months ago) |
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I agree, just simmer for 30 minutes, rinse very well, and then either roll in a towel or let it drain for a good 30 minutes.
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The_Chariot
Stranger


Registered: 07/10/06
Posts: 127
Last seen: 13 years, 7 months
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Re: WBS Confusion [Re: Sinthetic]
#5931109 - 08/04/06 11:07 AM (17 years, 5 months ago) |
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Just read the wbs tek on this site...it worked for me, wbs came out fine
-------------------- My will be ashes and a casket for all I want. The apocalypse, this way comes. So come. Sing the song. Repent kingdom, the end is here and it won't remain silent. The devil is in Atlanta. The army is surrounded. The civilians panic.
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vintage_gonzo
Stranger

Registered: 04/08/06
Posts: 457
Last seen: 15 years, 11 months
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the way that i have done and achieved %100 sccess is to open the bag and put the wbs in a collander. Wash with very hot water very thoroughly. Then fill 2/3 of a quart jar with wbs and pour water into the quart jar until it is full. Now let it sit for 24 hours, dump back into the collander and rinse very well. Now instead of letting sit after i rinse i put the wet wbs into the quart jar and add vermiculite as need so there is no standing water. This means there is more water in the wbs/verm mix without and puddles.
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HarvestTheBrain
CultDeVader

Registered: 07/14/03
Posts: 1,749
Loc: Usa
Last seen: 4 months, 10 days
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lol, everyone is pretty much different eh? well, i rinse in collander, soak for 12 hrs, dry for 30 min in collander over sink....
oh yeah, the seed i get has sunflower seeds in it, i usually jsut skim off the floaters, the others i leave in, IME i never had any problems with them, but if you feel safe the other way around, get the majority of them out
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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http://www.shroomery.org/forums/showflat.php/Number/3982155#Post3982155
Read the post above by "agar".
He doesn't simmer his wbs.
I think the reason is:
You can under or over simmer wbs. Either would not work well.
Sort of like an under or over baked cake.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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yup, each person has their own way of doing wbs.
each for their own reasons apparantly.
you want to hydrate your grains and kill the bacterial endospores.
do it however it works best for you. just remember the moisture content is quite important too.
oh, and what about grain clumping?
just find a tek that you feel covers all the bases and works best for your style.
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TrippinTeddy
Lost Voyager


Registered: 06/07/04
Posts: 18,461
Loc: Returning Video Tapes
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Thanks for all your help everyone.
I'm going to attempt this once again. I need it to work this time. At the end of my spore print.
BTW is it possible for a print to be contaminated?
Sounds stupid but I'm trying to rule everything out before I go this time. Its my last shot. Don't have money for more prints for a while.
-------------------- ToiletDuk said: For the record, I would show you my butthole but you would fall down and worship it as a God and you would give up everything to roam the land converting the heathens by fire and sword. Millions would die. No, no the cost is too great. I cannot.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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yes, it is very possible for a print to be dirty.
this is where agar comes in real handy.
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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>>>>>>>BTW is it possible for a print to be contaminated?
Certainly.
It most often depends on the expertise of who took it.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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TrippinTeddy
Lost Voyager


Registered: 06/07/04
Posts: 18,461
Loc: Returning Video Tapes
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On that note... how should I go about storing a print or using a print in that case... I have received a new print in the mail from a very generous shroomery member and I'm going to attempt again.
I fear the other may have been bad.
This is a great community. thanks everyone for your help. Good to see people helping people in this world
-------------------- ToiletDuk said: For the record, I would show you my butthole but you would fall down and worship it as a God and you would give up everything to roam the land converting the heathens by fire and sword. Millions would die. No, no the cost is too great. I cannot.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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When I use prints from trades or the wild, I use agar to get a clean culture.
you may have to try several dishes to isolate a clean piece, but that's a far better option than ruining everything.
you could just give it a try and hope to trust the source, but that is foolishness in my mind. especially if you only have that one print.
you could always test part of it, and upon contamination (if it happens), go back and start over with the agar work.
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TrippinTeddy
Lost Voyager


Registered: 06/07/04
Posts: 18,461
Loc: Returning Video Tapes
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Quote:
monstermitch said: When I use prints from trades or the wild, I use agar to get a clean culture.
you may have to try several dishes to isolate a clean piece, but that's a far better option than ruining everything.
you could just give it a try and hope to trust the source, but that is foolishness in my mind. especially if you only have that one print.
you could always test part of it, and upon contamination (if it happens), go back and start over with the agar work.
Next question... where can I get cheap supplies for agar work. I would pre-fer to go this route anyways to isolate the myc. My only prob is im kinda broke I could afford the potato dextrose.. but not sure about the containers... but i havent looked yet
-------------------- ToiletDuk said: For the record, I would show you my butthole but you would fall down and worship it as a God and you would give up everything to roam the land converting the heathens by fire and sword. Millions would die. No, no the cost is too great. I cannot.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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I gather them from all over.
no thrift store finds for agar work though, well, tippi will come up with something...
but for petri's and a loop and MEA and all that stuff, you'll just have to look around.
can't post non-vendor links, so try Sporeworks.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Quote:
Tippinthru said: http://www.shroomery.org/forums/showflat.php/Number/3982155#Post3982155
Read the post above by "agar".
He doesn't simmer his wbs.
I think the reason is:
You can under or over simmer wbs. Either would not work well.
Sort of like an under or over baked cake.
"Well Agar doesn't do it like that...."
For those who actually read the way I do it and didn't just skim through it:
1- it's not simmering, but steeping 2- not many, if any bursted seeds- again, because this ISN'T simmering 4- there will be no clumping because you RINSE the grains well to get the sludge off
So, I'm not totally disagreeing with Agar. I'm just saying you can reduce your soak time by 23-23 1/2 hours. If you sleep better at night knowing that you soaked for 24 instead of steeping for 1 GO FOR IT! More power to you! I've shown my results MANY times, so I'm not going to argue about which method I think is better.
And just a suggestion- if you are still having problems with endospores, check your PC times. 90 minutes at 15psi in a moderately loaded PC will more than suffice for WBS prepared this way.
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Quick WBS Prep
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Cubenisseur
Mad Props


Registered: 12/04/05
Posts: 1,392
Loc: Indian Land
Last seen: 13 years, 10 months
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Re: WBS Confusion [Re: FooMan]
#5932808 - 08/04/06 09:18 PM (17 years, 5 months ago) |
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Foo Mans Tek is da-bomb.
Simple-Quick-Easy-Effective


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Spacerace
Stranger
Registered: 08/03/06
Posts: 16
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Really though if you wanted to simmer just because in my experience i had trouble with the WBS not soaking enough. It couldn't of been the sludge because i would rinse it a bunch after steeping or simmering.
What I do for an real easy way to simmer is to just bring a large pot to a boil and then turn the stove heat off. And then dump the wild bird seed in there for a half hour.
But if you seeds do looked steeped enough skip this and just continue.
Oh yeah, use vermiculate to make sure it isn't too wet. The moisture should be in the seed but theres a lot of excess wetness left from whatever. Or you could just use a towel. Verm helps ime.
Edited by Spacerace (08/05/06 01:13 AM)
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: WBS Confusion [Re: Spacerace]
#5933739 - 08/05/06 04:00 AM (17 years, 5 months ago) |
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Quote:
Spacerace said: What I do for an real easy way to simmer is to just bring a large pot to a boil and then turn the stove heat off. And then dump the wild bird seed in there for a half hour.
Wow, I should try that!
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Quick WBS Prep
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