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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Surprising Results from a Test!
    #592906 - 03/29/02 02:22 PM (22 years, 4 days ago)

The two jars on the left were innoc'd on 3-22.
The two jars on the right were innoc'd on 3-23
Today is 3-29

They are all  BRF and innoc'd from the same
hawaiian syringe.
Anyone want to guess what I did differently?
I think I'll tell you tomorrow. Unless this thread
hopelessly sinks. :peace:

Free Spore Ring

   


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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Invisiblesloluva
....

Registered: 12/26/01
Posts: 2,752
Loc: Ohio
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592911 - 03/29/02 02:29 PM (22 years, 4 days ago)

Why would you wait?

Polyfill?


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Offlinebadjessejames
G HEAD

Registered: 03/30/01
Posts: 545
Loc: :) Passed out on my co...
Last seen: 18 years, 6 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592912 - 03/29/02 02:29 PM (22 years, 4 days ago)

If it's the same substarte and same syringe........
I would guess that you kept the ones on the right @ higher temps.....
Did I win...?


--------------------

"My generation played a really mean trick on me. I thought we were all
goofing off together, but it turns out everybody else went and got rich
while I was sleeping." Mission Hill

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: sloluva]
    #592925 - 03/29/02 02:46 PM (22 years, 4 days ago)

Sloluva, Because you love suspense. :cool:
And to see if anybody cares.
No polyfill, metal lids taped. (I haven't took the tape off yet)
Jesse, nope, they sat side by side in the incubator.
Anybody else?
:peace:

Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

Edited by WakingUpLate (03/29/02 03:01 PM)

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OfflineSurf Bum
member
Registered: 01/30/01
Posts: 158
Last seen: 21 years, 10 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592929 - 03/29/02 02:56 PM (22 years, 4 days ago)

did you sing to the ones on the right? or maybe you sang to the ones on the left, well which is it?


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Offlineroomusygloomush
Stranger
Registered: 03/04/02
Posts: 12
Last seen: 21 years, 10 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592931 - 03/29/02 03:01 PM (22 years, 4 days ago)

looks like you mighta sucked up some dextrose water into your syringe after the first innoculation, or honey water , something along those lines


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Offlinehappygrins
addict

Registered: 12/29/01
Posts: 588
Loc: Shroomery
Last seen: 19 years, 1 month
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592934 - 03/29/02 03:02 PM (22 years, 4 days ago)

Did you use freshly ground/Chunky BRF ?....

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InvisiblePennywise
Watch out now!
Male
Registered: 03/21/02
Posts: 60
Loc: Maryland
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592957 - 03/29/02 03:44 PM (22 years, 4 days ago)

Did you inject more into the ones on the right? I know when I injected more than 1cc in one and less than .5cc in the other the one with more than 1cc took off like crazy, more than double the speed.

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InvisibleDreaMaTrix
Shaman I am

Folding@home Statistics
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592958 - 03/29/02 03:47 PM (22 years, 4 days ago)

filter/filterdisk


--------------------





"We are the one's we have been waiting for" - Hopi saying

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InvisibleDreaMaTrix
Shaman I am

Folding@home Statistics
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
Re: Surprising Results from a Test! [Re: DreaMaTrix]
    #592962 - 03/29/02 03:49 PM (22 years, 4 days ago)

I know I'm right, thats why u have 'em upsidedown :wink:



--------------------





"We are the one's we have been waiting for" - Hopi saying

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Offlineknurd
enthusiast

Registered: 11/16/01
Posts: 330
Last seen: 7 years, 8 months
Re: Surprising Results from a Test! [Re: DreaMaTrix]
    #592999 - 03/29/02 04:20 PM (22 years, 4 days ago)

less water on the left....... more water on the right?


--------------------
PLEASE DON'T PM ME FOR FREE SPORES I GAVE AT THE OFFICE

Now I will trade when I can

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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 3 months
Re: Surprising Results from a Test! [Re: knurd]
    #593002 - 03/29/02 04:23 PM (22 years, 4 days ago)

did you sit on the ones on the right?
are you a goose?


--------------------
blasted holes into night until she bled sunshine

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Offlinebobobobob
Stranger
Registered: 02/27/02
Posts: 17
Last seen: 22 years, 4 days
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593013 - 03/29/02 04:31 PM (22 years, 4 days ago)

Ground up golf discs from hobbyxpress.com? Water absorbent polymer crystals? Come on, let us know. I'm innoculating some jars tonight and I want to try this.

Bob

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InvisiblePinhead
Oregano
Male

Registered: 05/13/00
Posts: 1,819
Loc: Hootersville
Re: Surprising Results from a Test! [Re: DreaMaTrix]
    #593019 - 03/29/02 04:34 PM (22 years, 4 days ago)

This is cruel,just tell us already!

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: Pinhead]
    #593045 - 03/29/02 04:51 PM (22 years, 4 days ago)

The first thing you must learn in cultivating mushrooms is patience.
Ok let me find the thread that gave me the crazy idea, and I'll
bump it. It will take a few minutes, so be patient.
:peace: 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 3 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593062 - 03/29/02 05:14 PM (22 years, 4 days ago)

so let me get this straight.
you add your water to your brf after you steralize?
and you add this steralized water by injecting it through your 4 inoculation holes?
wouldn't this cause contamination problems...i mean, it would distribut the spores much better, but it wouldn't distribut the water content evenly???
is any of this making sense? did i read that right?


--------------------
blasted holes into night until she bled sunshine

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593067 - 03/29/02 05:20 PM (22 years, 4 days ago)

Pennywise wins, I injected more solution. All of the water as a
matter of fact. I mixed up my brf and verm dry. And PC'd them
dry. Then I took the water that I would have mixed, and mixed
it with the spores(after I boiled and cooled it of course), then
injected 12 cc's into each hole. Half toward the glass and half
toward the center.
So If you're innoculating tonight, it may be too late for you to try
this. Sorry.
It has also occured to me that it may work to bake the dry jars
to steralize. This could help those of you without a PC. Just
an idea.
I didn't expect this to work as well as it did. And I don't reccomend
straying from the PF Tek, unless you're willing to accept total
loss once in a while. But this time it seems to have worked.
Pennywise, I owe you a prize. I have a nice hawaiian print
for you if you want it.
Hope that helps you guys!
Never Stop Learning!
:peace:


Free Spore Ring
 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

Edited by WakingUpLate (03/30/02 10:04 AM)

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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 3 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593076 - 03/29/02 05:28 PM (22 years, 4 days ago)

what about the water consitancy in your jars tho...
you may have problems with fruiting...i hope not tho...its a great idea.
I was think about somthing along the lines of this the other day.
Instead of mixing spores with water, inoculate a mass amount of honey water and when you want to inoculate your jars (steralized dry, like you did) just take the lids off and pour a crap load of it in...you wouldn't need to worry about contamination because you would be putting so much mycel. into the jars there would be no compitiion for other bacteria.
just a thought
hey. you wanna send me a print to!


--------------------
blasted holes into night until she bled sunshine

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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 18 days, 15 hours
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593098 - 03/29/02 05:51 PM (22 years, 4 days ago)

Something similar is described in this method:

http://www.shroomery.org/findorgrowthem/neimad.html

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: Anno]
    #593125 - 03/29/02 06:26 PM (22 years, 4 days ago)

Thanks Anno, I had a feeling this would lead to baking. I guess
it already has. Man, you already know all this shit don't you
dude. So why isn't it mentioned much?  That to me is an
indicator that it might not be as failsafe as the PF Tek. I have
a feeling I'm going to find out in the coming weeks. A few points
I thought I should mention:
1)After you inject the water, you shake the jars.
2)The mycelia is not pulled back off the glass like it normally is
when a cake is covered.
3)I only risked 4 jars to try this. 2 with metal lids, 2 with tyvex.(exact same results)

I hope bringing this method up doesn't cause a bunch of
confusion in the future, But it probably will.
Just trying to help. :peace: 


Free Spore Ring

   


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

Extras: Filter Print Post Top
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