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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Surprising Results from a Test!
    #592906 - 03/29/02 02:22 PM (22 years, 6 months ago)

The two jars on the left were innoc'd on 3-22.
The two jars on the right were innoc'd on 3-23
Today is 3-29

They are all  BRF and innoc'd from the same
hawaiian syringe.
Anyone want to guess what I did differently?
I think I'll tell you tomorrow. Unless this thread
hopelessly sinks. :peace:

Free Spore Ring

   


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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Invisiblesloluva
....

Registered: 12/26/01
Posts: 2,752
Loc: Ohio
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592911 - 03/29/02 02:29 PM (22 years, 6 months ago)

Why would you wait?

Polyfill?


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Offlinebadjessejames
G HEAD

Registered: 03/30/01
Posts: 545
Loc: :) Passed out on my co...
Last seen: 19 years, 1 month
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592912 - 03/29/02 02:29 PM (22 years, 6 months ago)

If it's the same substarte and same syringe........
I would guess that you kept the ones on the right @ higher temps.....
Did I win...?


--------------------

"My generation played a really mean trick on me. I thought we were all
goofing off together, but it turns out everybody else went and got rich
while I was sleeping." Mission Hill

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: sloluva]
    #592925 - 03/29/02 02:46 PM (22 years, 6 months ago)

Sloluva, Because you love suspense. :cool:
And to see if anybody cares.
No polyfill, metal lids taped. (I haven't took the tape off yet)
Jesse, nope, they sat side by side in the incubator.
Anybody else?
:peace:

Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

Edited by WakingUpLate (03/29/02 03:01 PM)

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OfflineSurf Bum
member
Registered: 01/30/01
Posts: 158
Last seen: 22 years, 5 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592929 - 03/29/02 02:56 PM (22 years, 6 months ago)

did you sing to the ones on the right? or maybe you sang to the ones on the left, well which is it?


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Offlineroomusygloomush
Stranger
Registered: 03/04/02
Posts: 12
Last seen: 22 years, 5 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592931 - 03/29/02 03:01 PM (22 years, 6 months ago)

looks like you mighta sucked up some dextrose water into your syringe after the first innoculation, or honey water , something along those lines


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Offlinehappygrins
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Registered: 12/29/01
Posts: 588
Loc: Shroomery
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Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592934 - 03/29/02 03:02 PM (22 years, 6 months ago)

Did you use freshly ground/Chunky BRF ?....

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InvisiblePennywise
Watch out now!
Male
Registered: 03/21/02
Posts: 60
Loc: Maryland
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592957 - 03/29/02 03:44 PM (22 years, 6 months ago)

Did you inject more into the ones on the right? I know when I injected more than 1cc in one and less than .5cc in the other the one with more than 1cc took off like crazy, more than double the speed.

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InvisibleDreaMaTrix
Shaman I am

Folding@home Statistics
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #592958 - 03/29/02 03:47 PM (22 years, 6 months ago)

filter/filterdisk


--------------------





"We are the one's we have been waiting for" - Hopi saying

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InvisibleDreaMaTrix
Shaman I am

Folding@home Statistics
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
Re: Surprising Results from a Test! [Re: DreaMaTrix]
    #592962 - 03/29/02 03:49 PM (22 years, 6 months ago)

I know I'm right, thats why u have 'em upsidedown :wink:



--------------------





"We are the one's we have been waiting for" - Hopi saying

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Offlineknurd
enthusiast

Registered: 11/16/01
Posts: 330
Last seen: 8 years, 3 months
Re: Surprising Results from a Test! [Re: DreaMaTrix]
    #592999 - 03/29/02 04:20 PM (22 years, 6 months ago)

less water on the left....... more water on the right?


--------------------
PLEASE DON'T PM ME FOR FREE SPORES I GAVE AT THE OFFICE

Now I will trade when I can

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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 10 months
Re: Surprising Results from a Test! [Re: knurd]
    #593002 - 03/29/02 04:23 PM (22 years, 6 months ago)

did you sit on the ones on the right?
are you a goose?


--------------------
blasted holes into night until she bled sunshine

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Offlinebobobobob
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Registered: 02/27/02
Posts: 17
Last seen: 22 years, 6 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593013 - 03/29/02 04:31 PM (22 years, 6 months ago)

Ground up golf discs from hobbyxpress.com? Water absorbent polymer crystals? Come on, let us know. I'm innoculating some jars tonight and I want to try this.

Bob

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InvisiblePinhead
Oregano
Male

Registered: 05/13/00
Posts: 1,819
Loc: Hootersville
Re: Surprising Results from a Test! [Re: DreaMaTrix]
    #593019 - 03/29/02 04:34 PM (22 years, 6 months ago)

This is cruel,just tell us already!

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InvisibleWakingUpLate
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Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: Pinhead]
    #593045 - 03/29/02 04:51 PM (22 years, 6 months ago)

The first thing you must learn in cultivating mushrooms is patience.
Ok let me find the thread that gave me the crazy idea, and I'll
bump it. It will take a few minutes, so be patient.
:peace: 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 10 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593062 - 03/29/02 05:14 PM (22 years, 6 months ago)

so let me get this straight.
you add your water to your brf after you steralize?
and you add this steralized water by injecting it through your 4 inoculation holes?
wouldn't this cause contamination problems...i mean, it would distribut the spores much better, but it wouldn't distribut the water content evenly???
is any of this making sense? did i read that right?


--------------------
blasted holes into night until she bled sunshine

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593067 - 03/29/02 05:20 PM (22 years, 6 months ago)

Pennywise wins, I injected more solution. All of the water as a
matter of fact. I mixed up my brf and verm dry. And PC'd them
dry. Then I took the water that I would have mixed, and mixed
it with the spores(after I boiled and cooled it of course), then
injected 12 cc's into each hole. Half toward the glass and half
toward the center.
So If you're innoculating tonight, it may be too late for you to try
this. Sorry.
It has also occured to me that it may work to bake the dry jars
to steralize. This could help those of you without a PC. Just
an idea.
I didn't expect this to work as well as it did. And I don't reccomend
straying from the PF Tek, unless you're willing to accept total
loss once in a while. But this time it seems to have worked.
Pennywise, I owe you a prize. I have a nice hawaiian print
for you if you want it.
Hope that helps you guys!
Never Stop Learning!
:peace:


Free Spore Ring
 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

Edited by WakingUpLate (03/30/02 10:04 AM)

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OfflineAlpheratus
member
Registered: 03/23/02
Posts: 171
Loc: neo berlin
Last seen: 17 years, 10 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593076 - 03/29/02 05:28 PM (22 years, 6 months ago)

what about the water consitancy in your jars tho...
you may have problems with fruiting...i hope not tho...its a great idea.
I was think about somthing along the lines of this the other day.
Instead of mixing spores with water, inoculate a mass amount of honey water and when you want to inoculate your jars (steralized dry, like you did) just take the lids off and pour a crap load of it in...you wouldn't need to worry about contamination because you would be putting so much mycel. into the jars there would be no compitiion for other bacteria.
just a thought
hey. you wanna send me a print to!


--------------------
blasted holes into night until she bled sunshine

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OfflineAnnoA
Experimenter
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Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593098 - 03/29/02 05:51 PM (22 years, 6 months ago)

Something similar is described in this method:

http://www.shroomery.org/findorgrowthem/neimad.html

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: Anno]
    #593125 - 03/29/02 06:26 PM (22 years, 6 months ago)

Thanks Anno, I had a feeling this would lead to baking. I guess
it already has. Man, you already know all this shit don't you
dude. So why isn't it mentioned much?  That to me is an
indicator that it might not be as failsafe as the PF Tek. I have
a feeling I'm going to find out in the coming weeks. A few points
I thought I should mention:
1)After you inject the water, you shake the jars.
2)The mycelia is not pulled back off the glass like it normally is
when a cake is covered.
3)I only risked 4 jars to try this. 2 with metal lids, 2 with tyvex.(exact same results)

I hope bringing this method up doesn't cause a bunch of
confusion in the future, But it probably will.
Just trying to help. :peace: 


Free Spore Ring

   


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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OfflineLSD_4me
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Registered: 02/09/02
Posts: 416
Last seen: 22 years, 5 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593158 - 03/29/02 08:06 PM (22 years, 6 months ago)

its water amount... that affects growth in a huge way

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Invisibleshaggymane
PHARMER

Registered: 03/11/02
Posts: 514
Loc: great white north
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593169 - 03/29/02 08:21 PM (22 years, 6 months ago)

nice test
handstand

hey! is that tyvex form the post office
hahahahaha

Edited by shaggymane (03/29/02 08:25 PM)

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InvisibleDreaMaTrix
Shaman I am

Folding@home Statistics
Registered: 02/11/02
Posts: 3,125
Loc: Falling into place
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593449 - 03/30/02 05:08 AM (22 years, 6 months ago)

Would this not leave the rice uncooked??? Rice needs to soak up water when its cooking, surely??



--------------------





"We are the one's we have been waiting for" - Hopi saying

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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #593743 - 03/30/02 02:07 PM (22 years, 6 months ago)

Very interesting bro.

But what worries me is.... that you don't have any water in the jars before you pc them.
How does the rice cook ?
I guess i am missing something....lolz
Woooshhhhh right over my head.....*blonde moment* maybe


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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InvisibleNighted
Ghost

Registered: 11/09/01
Posts: 698
Loc: Funkytown
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #594579 - 03/31/02 12:17 PM (22 years, 6 months ago)

Just use a cloned strain and inoculate via liquid culture. You'll see the same results and better.


--------------------

Freedom defined is freedom denied.

Nighted is better than Google. Please take time to rate 5/5. Thanks!

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OfflineBaby_Hitler
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Re: Surprising Results from a Test! [Re: WakingUpLate]
    #616980 - 04/24/02 01:05 AM (22 years, 5 months ago)

So how `bout an update on your progress with this drytek project?


--------------------
This space for rent

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: Baby_Hitler]
    #617122 - 04/24/02 06:43 AM (22 years, 5 months ago)

Four jars were taken and cased into a half gallon juice
box: 2" damp verm, 4"substrate, 1" damp verm on top.
This pic was taken on 4-19 (27 days after innoculation) 

Not bad huh?
:peace:
Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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Offlinepsychomyco
Stranger
Registered: 04/25/02
Posts: 4
Loc: MI
Last seen: 22 years, 5 months
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #618338 - 04/25/02 11:31 AM (22 years, 5 months ago)

did you just crumble the cakes over the 1st layer, let them colonize a bit, then cover with the third layer?


--------------------
I breed chick-a-loos

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: psychomyco]
    #618381 - 04/25/02 12:16 PM (22 years, 5 months ago)

"did you just crumble the cakes over the 1st layer, let them colonize a bit, then cover with the third layer? "
No, Since I wasn't spawning more substrate, it only had to
recover(grow back together), and did it relatively fast.
I added the damp verm, then crumbled the cakes, pressed
lightly to even it up, Then immediately covered loosely with
with damp verm (no packing at all!). Back to the incubator
for 2-3 days, About three spots the size of a dime were
spotted, so it was removed, and placed in a high humidity
terrarium (for cakes) until pinning occured. It was then placed
on the perlite (in the pic) with a clear cover similar to one of
those bottled water fountain bottles ( I don't know what
they're called) , and fanned three times a day.
The second flush is about an inch tall right now. I wish I had
of put it back in the other terrarium to induce a second pinset.
I think it would be further along now if I had.
Hope that helps.
:peace:
Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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InvisibleMr. Pink
Model Prisoner
Registered: 01/29/01
Posts: 142
Loc: Small town with a BIG pro...
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #619068 - 04/26/02 02:57 AM (22 years, 5 months ago)

Cool Experiment, but why did you mix your controls? That last pic is all four jars right, or am I missing something? Seems like a waste of an experiment to me. Besides for that it was a real cool idea, even if it has been thought of before. New spins on old ideas and simple shit that makes you go "why has no one thought of that before?" keep this hobby evolving....

You should have fruited the jars seperately to discover the effect of water content and distribution on pinset, flush potential, .etc. Maybe I'll get off my lazy ass and do it...or maybe not. Nice try though man! Keep up the cool ideas and make a breakthrough. A huge one is right around the corner.... I CAN FEEL IT :wink:


--------------------
_________________________________________

"Why do I get to be Mr. Pink?, I sound like a fucking pussy."

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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Surprising Results from a Test! [Re: WakingUpLate]
    #619158 - 04/26/02 06:23 AM (22 years, 5 months ago)

not to be a dick, but i think that 4 cakes fruited as individuals would yield alot more than what i see here....


--------------------
Admin @ mycotopia.net
Mycotopia

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InvisibleWakingUpLate
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Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Surprising Results from a Test! [Re: Mr. Pink]
    #619273 - 04/26/02 08:58 AM (22 years, 5 months ago)

You're right man, mixing the controls would have been a bad idea. Not that it wouldn't work, but it would have told me less. I didn't. I just didn't mention that I had tried this on four jars.
And Hippie, I wondered about that myself. So the next four, I birthed as cakes. You know how a lot of times The shrooms on a cake seems to concentrate in little areas? Well these didn't. They grew like tiny hairs all over. Then about the time the veils dropped(largest cap was about3/4"), 3 of 4 cakes simultaniously contaminated. So fruit and cakes were tossed. I'm still kinda wondering about that one. I think I might have birthed them too soon. And the more aggressive mycelia didn't have time to dominate the less aggressive due to such even spore distribution. But that's just my guess. Hell I'm still learning. Have been all my life. Thanks for everyone's input. I never thought this thread would live this long.
:peace:
Free Spore Ring
 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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