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OfflineExplosiveMango
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Cloning question
    #5928686 - 08/03/06 02:10 PM (17 years, 5 months ago)

Just out of curiosity...
If I take a section of my favorite mushroom, and use it to start a new liquid culture-

Will this new mycelium mass be haploid or diploid?
(when during fruiting do the multiple nuclei merge?)


Also, if I have a genetic sample of a polykaryotic mycelium, is there risk of these genetic partners 'breaking up' at some juncture?




(should this be in advanced?)


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Know your self.
Know your substance.
Know your source.

The most distorted perspective possible is the perspective that yours is not distorted.


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OfflineRogerRabbitM
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Re: Cloning question [Re: ExplosiveMango]
    #5928723 - 08/03/06 02:23 PM (17 years, 5 months ago)

It will be dikaryotic. I haven't ever heard of dikaryons separating back into monokaryotic mycelium. Of course that doesn't prove it impossibe, but I currently know of none that do that. Perhaps workman will see this and add something from his experiences.


>(should this be in advanced?)

No, it belongs here in my opinion.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineExplosiveMango
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Re: Cloning question [Re: RogerRabbit]
    #5929410 - 08/03/06 06:39 PM (17 years, 5 months ago)

Alright.. well I'll noob it down a bit by also requesting this information:

What determines how many genetic components a cubensis mycelium mass will have?

Does it top out at two? Or some other number?
Or is it simply less likely to have above a certain number? (what number?)


--------------------
Know your self.
Know your substance.
Know your source.

The most distorted perspective possible is the perspective that yours is not distorted.


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OfflineExplosiveMango
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Re: Cloning question [Re: ExplosiveMango]
    #5930480 - 08/04/06 04:18 AM (17 years, 5 months ago)

...bump


--------------------
Know your self.
Know your substance.
Know your source.

The most distorted perspective possible is the perspective that yours is not distorted.


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OfflineRogerRabbitM
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Re: Cloning question [Re: ExplosiveMango]
    #5930600 - 08/04/06 06:28 AM (17 years, 5 months ago)

With multispore inoculation, often thousands of strains are created. By anastamosis, most merge into one strain by the time fruiting comes, but since some strains are not compatable, you often end up with several in the same tray. This is why sometimes we see a project with drastically different looking fruits at the same time. There's an excellent section on this in The Mushroom Cultivator.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflineWorkmanV
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Re: Cloning question [Re: RogerRabbit]
    #5931111 - 08/04/06 11:07 AM (17 years, 5 months ago)

There are methods haploidization using certain chemicals (benomyl). I haven't looked into it much but here is a relevant shroomery post.

Shroomery post


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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InvisibleRoadkillM
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Re: Cloning question [Re: Workman]
    #5931161 - 08/04/06 11:21 AM (17 years, 5 months ago)

bro,

that link isn't working for me!~

anyone else see it?


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisiblecreamcorn
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Re: Cloning question [Re: Roadkill]
    #5931170 - 08/04/06 11:24 AM (17 years, 5 months ago)



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InvisibleRoadkillM
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Re: Cloning question [Re: creamcorn]
    #5931177 - 08/04/06 11:27 AM (17 years, 5 months ago)

thanks cc!~

that one worked.

:smile:


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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