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Offlinefromthemoon
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Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide?
    #5925262 - 08/02/06 12:25 PM (17 years, 5 months ago)

Hello,

I'm wondering which disinfectants are best and/or easiest to work with:

1) 10% bleach/water
2) Rubbing Alcohol
3) Hydrogen Peroxide?

Now I asked my local pharmacist which of the three was best. I said I have to kill parasites (which are way harder than bacterial - they are multi-celled with a hard protective coating, cysts). She said 10% Bleach Water.

I also asked how long the bleach water needs to be exposed to kill parasites. She didn't know, but said at least 1/2 hour. Asked the same for bacteria and she said just a few minutes max, it's almost instant.

So I feel 10% bleach water is easiest and also safest in a glove box w/ flame (bleach water is not flammable).

But... I'm wondering if a STRONG BLEACH SMELL within a glove box will harm the spores when making syringes (especially) or inoculating jars.

What do you guys use and why!

Thanks


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Offlinetonyperez420
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925290 - 08/02/06 12:36 PM (17 years, 5 months ago)

I would spray down the GB with your bleached water, wait 15 mins for things to settle down (cause its the strongest disinfectant)

I would have a rubbing alcohol spray bottle already in the GB before you spray bleached water... after the 15 mins, you could use that rubbing alcohol spray within your GB to disinfect anything else you need already in the glove box, needle, etc...
(optional, I would also try to keep a lighter in there, but caution is needed cause rubbing alcohol is flammable

I would use hydrogen peroxide ONLY in my water to prevent contams in my imagine tropic aire humidifiern OR a small amount to spray on your perlite if its in your FC

hope that helps

GL


Edited by tonyperez420 (08/02/06 12:39 PM)


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InvisibleTippinthru
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925338 - 08/02/06 12:48 PM (17 years, 5 months ago)

Read the 2 links below about BLEACH.
http://www.moldacrossamerica.org/notobleach.htm

http://www.epa.gov/pesticides/factsheets/chemicals/bleachfactsheet.htm

Bleach is NOT particularly effective against molds, and mold spores.

Lemon scent Lysol is my choice, for large area's.

You can buy it in 1, 2 & 4 quart bottles.
Then, pour in a trigger mist bottle.

For cleansing air, Oust, is the best.

For needles, small metal tools, glass, etc, as a quick sanitizer.
I use 70% alcohol.

Just my $0.02 cents


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Perfection is attained by slow degrees; it requires the hand of time...
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Offlinefromthemoon
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: Tippinthru]
    #5925382 - 08/02/06 01:07 PM (17 years, 5 months ago)

OK - I read the links, makes sense.

I bought Oust for the air. I'm assuming that is for when you are taking jars out of the pressure cooker etc. and into the box, i.e. it's used mainly for the air in the room - right?

I also got 70% alcohol for dipping metal tools before flaming.

I'm worried about Lysol being flammable which is why I was hoping bleach water would work (I already bough Lysol too).

How do you use Lysol in an air tight glove box without blowing yourself up?


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Invisiblefastfred
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: Tippinthru]
    #5925386 - 08/02/06 01:07 PM (17 years, 5 months ago)

From the EPA site...

"Registered liquid bleach products contain sodium hypochlorite, a compound used as a cleaner and to kill bacteria, fungi, and viruses."

"Because bleach is effective against a wide range of bacteria, fungi, and viruses, EPA has registered sodium hypochlorite for use in the sanitization and disinfection of household premises, food processing plants, and agricultural settings. Bleach is also used in animal facilities, hospitals, and human drinking water supplies."


Bleach is highly effective at killing all forms of life, but is also very safe to use for larger organisms such as ourselves.

10% bleach is what is always used in labs as a surface sterilizer. The order you have them ranked in your list is a good order of effectiveness. Bleach is far more effective than alcohol, which is also much more effective than hydrogen peroxide.


-FF


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Invisiblefastfred
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fastfred]
    #5925391 - 08/02/06 01:10 PM (17 years, 5 months ago)

> How do you use Lysol in an air tight glove box without blowing yourself up?

You don't. Or you avoid sparks and flame.

> I was hoping bleach water would work

It will. It's the most effective surface sterilizer you can use. Pay no mind to crackpot websites that discuss mold in drywall rather than surface sterilization.


-FF


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Offlinefromthemoon
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fastfred]
    #5925397 - 08/02/06 01:13 PM (17 years, 5 months ago)

Hmmm...

I have had mold in the bathroom and used 100% bleach, only to have it return months later.

But...

I'd rather use bleach so I don't blow-up.

The Pharm lady also said that order was strongest to weakest.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Offlinefromthemoon
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Posts: 353
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925409 - 08/02/06 01:16 PM (17 years, 5 months ago)

"Pay no mind to crackpot websites that discuss mold in drywall rather than surface sterilization."

I was thinking the same thing - Glass/Plasic/Metal are a far cry from "drywall".

What about bleach fumes?


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Invisiblefastfred
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925428 - 08/02/06 01:22 PM (17 years, 5 months ago)

> I have had mold in the bathroom and used 100% bleach, only to have it return months later.

Yeah, a light spraying of bleach isn't going to kill the mold deep in the drywall, or the mold on the studs behind it, so on and so forth. Bleach will kill anything that it comes in direct contact with.

> What about bleach fumes?

Avoid breathing them. 10% bleach fumes aren't that strong though, I highly doubt the fumes from a 10% solution will kill anything.


-FF


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OfflineCoolMojo
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fastfred]
    #5925629 - 08/02/06 02:30 PM (17 years, 5 months ago)

hmm, I always figured 10% was used in lab/general hospital cleaning due to cost. To bath a room in bleach is a hell of alot cheaper then doing it with alcohol. The other thing to consider is the rate of evaporation. I was under the impression that alcohol was more effective but only in smaller surface areas that you can keep bathed in it, where as bleach water will stay on the surface much longer so its better for practical reasons.
Maybe there is more to it then that, I don't know.


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OfflineRogerRabbitM
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: CoolMojo]
    #5925703 - 08/02/06 02:50 PM (17 years, 5 months ago)

They have different purposes. Bleach is not effective against fungi. I regularly dip cultures from the wild into ten percent bleach before transferring to agar to kill mold spores and bacteria, but it doesn't harm the live culture. That's why spraying bleach on the black mold around your bathtub or window sills does nothing.

Iodine is very effective against microbes. When I clone, I wash the whole mushroom with iodine before cutting out a section to transfer, which is then dipped into ten percent bleach.

Peroxide will help prevent mold in your humidifiers. Many experienced mycologists also use peroxide to dip cultures before transferring to agar. I prefer not to use it for that purpose except for when transferring dry tissue because of the damage it causes at the cellular level, which the fungi must then recover from.

Alcohol is great for cleaning tools, tabletops and gloves before doing sterile work, but you should still flame sterilize scalpels and needles, etc. A well equipped lab will have a multitude of cleaning agents, as well as at least two fire extinguishers at opposite ends of the working area. I don't know of any particular one cleaning agent that will do it all. I hope this helps.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinefromthemoon
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Registered: 08/02/06
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: CoolMojo]
    #5925710 - 08/02/06 02:52 PM (17 years, 5 months ago)

sounds like bleach is OK then.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Invisiblefastfred
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: CoolMojo]
    #5925719 - 08/02/06 02:54 PM (17 years, 5 months ago)

Bleach is more effective. It also kills a much wider array of organisms, including viri. It's not just used because it's cheap. Lysol is probably almost as effective, but it leaves a residue which may or may not be desirable in certain settings.

Bleach water is what they use to hose off bio-suits before the people inside come out of them. Believe me if there was something more effective than bleach the people stepping out of an ebola contaminated bio-suit would use it.

Alcohol is used at 70% to slow it's evaporation and because the water in it helps drive the alcohol into cells to kill them.


-FF


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Offlinefromthemoon
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925731 - 08/02/06 02:57 PM (17 years, 5 months ago)

OK - so some people use lysol because it has several chemicals and covers more bases?

If I'm flaming, I won't use lysol and alcohol only for wiping.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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Offlinefromthemoon
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Registered: 08/02/06
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925737 - 08/02/06 02:59 PM (17 years, 5 months ago)

Intuitively, I feel bleach is best and most natural for spraying down a glove box.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!


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OfflineRogerRabbitM
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fromthemoon]
    #5925753 - 08/02/06 03:05 PM (17 years, 5 months ago)

Quote:

fromthemoon said:
Intuitively, I feel bleach is best and most natural for spraying down a glove box.




Good choice, but a damp paper towell will do just as well unless your last project was contaminated. Remember, you don't dump your spawn out on the floor of the glovebox. All you're looking for is a very still air environment so the air currents don't blow dust and other nasties inside your jars or petri dishes. It also helps to have the floor of the glovebox damp, as any particles that land there will stay put.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblefastfred
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: RogerRabbit]
    #5925761 - 08/02/06 03:07 PM (17 years, 5 months ago)

Quote:

RogerRabbit says:
Bleach is not effective against fungi. I regularly dip cultures from the wild into ten percent bleach before transferring to agar to kill mold spores and bacteria, but it doesn't harm the live culture.




Quote:

The EPA says:
"Registered liquid bleach products contain sodium hypochlorite, a compound used as a cleaner and to kill bacteria, fungi, and viruses."
[...]
"Because bleach is effective against a wide range of bacteria, fungi, and viruses, EPA has registered sodium hypochlorite for use in the sanitization and disinfection of household premises, food processing plants, and agricultural settings. Bleach is also used in animal facilities, hospitals, and human drinking water supplies."
[...]
"You wouldn't think that ordinary chlorine bleach is a pesticide - but it is. Because it kills bacteria- and viruses it is called a disinfectant or an antimicorbial pesticide. And because it kills fungi and molds it is also known as a fungicide."






Quote:

The CDC says:
How can people decrease mold exposure?

Sensitive individuals should avoid areas that are likely to have mold, such as compost piles, cut grass, and wooded areas. Inside homes, mold growth can be slowed by keeping humidity levels between 40% and 60%, and ventilating showers and cooking areas. If there is mold growth in your home, you should clean up the mold and fix the water problem. Mold growth can be removed from hard surfaces with commercial products, soap and water, or a bleach solution of no more than 1 cup of bleach in 1 gallon of water.






Maybe RR got his bleach from the same place he got those bunk shiitake spores?

Note: I'll stop giving you shit about your claim that shiitake spores can't grow mushrooms as soon as you admit that you were wrong and I was right, and that I won your "challenge" to prove that shiitake spores are viable.


-FF


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InvisibleBlutjager
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fastfred]
    #5926774 - 08/02/06 08:34 PM (17 years, 5 months ago)

Unless you like the idea of having all the skin on your hands go up in flames do yourself a favor and DON'T spray oust in a glove box if you plan on flame sterilizing anything in there.Yes I am speaking from experience and it is no fun whatsoever,I have since than only misted everything down with alcohol and than just wait a little while for it all to evaporate,Oust lights up like a bomb


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InvisibleHairyPotter
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: fastfred]
    #27962442 - 09/22/22 12:39 PM (1 year, 4 months ago)

IS the 10% bleach just for the GB or can it be used to prep the monotub too? Or should 70% RA be used for that?

My only concern is if the bleach leaves behind a residue (where RA evaporates fairly quickly) that could harm the grow.


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OfflineKROM
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: HairyPotter] * 1
    #27962450 - 09/22/22 12:42 PM (1 year, 4 months ago)



Also 70% isopropyl alcohol for sanitation.


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Offlinehazyhorse
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: HairyPotter] * 2
    #27962466 - 09/22/22 12:55 PM (1 year, 4 months ago)

KROM said it best, 70% iso is best. bursts cel walls better bc of the water content, it’s actually better than 91% at sanitizing for our purposes. you don’t need to do anything more than washing the tub with soap & water but you can rub it down with ISO if it makes you feel better. spawning to tubs is inherently dirty, there are trillions of contaminate spores in the air at any given time, unless you spawn in a sterile HEPA filtered room & sterilize every single thing you’re using to spawn, you’re gonna have mold spores in there. your spawn being clean is what determines if you get contamination, not if there was some random trich spore in the air

this thread is old as fuck, try to check the post dates when looking through threads & posting. necroing isn’t horrible if it’s a relevant post, but a lot of info that’s older than like 8 years is super outdated. when using the search function, limit your search to threads newer than 7 years. a lot has changed in mush cult since then.


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OnlineThe Tao
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: KROM] * 1
    #27962591 - 09/22/22 02:14 PM (1 year, 4 months ago)

Quote:

KROM said:


Also 70% isopropyl alcohol for sanitation.



Happy 666th post, KROM!


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OfflineHappinessStan
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Re: Which disinfectant? 10% bleach OR Rubbing Alcohol OR Hydrogen Peroxide? [Re: HairyPotter]
    #27962615 - 09/22/22 02:36 PM (1 year, 4 months ago)

It wouldn't hurt to look at how old threads were before you decide to revive them...


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