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Cepheus
Balance



Registered: 04/19/06
Posts: 8,266
Loc: the space between reality...
Last seen: 1 year, 1 month
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Best spore printing tek
#5922899 - 08/01/06 07:27 PM (17 years, 5 months ago) |
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My casing has some very tasty looking mushrooms that veils have just torn.
What is the easiest way to take a sterile enough spore print to be used in a syringe at a later date? I possess no special equipment (like gloveboxes etc)
I have taken prints before but have been unsuccessful at taking a sterile one
-------------------- "I only ever hope to reach equilibrium, in Nature's matrix, in line with the meridian" ~ Jehst
"...and I know that I have to keep breathing, as tomorrow the sun will rise, who knows what the tide will bring?" Free Spore Ring Europe Send any spare spore prints you might have and help the distribution
Open Source. Freedom. GNU/Linux Addicting is not a word.
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: Best spore printing tek [Re: Cepheus]
#5923112 - 08/01/06 08:36 PM (17 years, 5 months ago) |
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small closed room, properly sterilized. wipe a sheet of foil with alcohol to sterlize it, place the caps on the foil, then cover each cap with a sterilized (with alcohol) shot glass (or drinking glass, w/e)
wait 24 hours, re-sterilize the small closed room, wear sterile gloves, pick up the shot glass, carefully remove cap, fold foil over the top of the print, fold the edges in so it's a sealed little square, then do that for every print you're taking.
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civility
Stranger

Registered: 01/17/14
Posts: 43
Last seen: 7 years, 11 months
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Re: Best spore printing tek *DELETED* [Re: liamtheloser]
#19954235 - 05/07/14 02:01 PM (9 years, 8 months ago) |
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Post deleted by civilityReason for deletion: N/A
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MushroomWizard420
mycological magician



Registered: 04/03/14
Posts: 307
Loc: space
Last seen: 1 year, 9 months
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Re: Best spore printing tek [Re: civility]
#19954434 - 05/07/14 02:42 PM (9 years, 8 months ago) |
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Quote:
civility said: do i need to "dry", in a sense, the print before i fold down the edges? or will the print be relatively dry enough?
the alcohol will evaporate off of the foil real quick, definitely before the mushroom is done printing itself, so it should need dried anymore unless you do it underwater. and don't do that^
-------------------- "And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good." -Shroomesis 4:20  MushroomWizard420 The spirit of the forest watches over his children as they grow...
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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That method is totally fucked.
First, use a SAB. You can't get away with making clean prints without one.
Second, you're not gonna be sterilizing a room without dropping thousands of dollars.
Third, wiping foil (which is already sterile) with alcohol is like washing your face then wiping shit on it. Alcohol doesn't sterilize anything, it only sanitizes. Big difference.
What you need to do is make a SAB. Take your roll of foil in there (after wiping the outside with alcohol) and rip off the first foot or so. Throw that away, it's touched open air and is dirty.
Take the next little bit of foil (however much you need depends on how many prints you want) and place it on the bottom of your SAB., shiny side up.
Take a paper clip and bend it so you have a little hook. Wipe this with alcohol and hook your cap from the top, for a little handle. Using alcohol-wiped scissors, cut the cap off as close to the gills as you can get without touching them. Then use your hook to transfer the cap onto your foil. Repeat until you're done.
Seal up your SAB and leave it to drop for 24 hours. After that time, pull the caps out and wait another 12-24 hours so it dries a bit. After they dry, take a brand new razor or scalpel in there and cut each print apart. From there I like to just bag them up open-faced in little zipper-top bags. Not sandwich bags, those are too big, look in the craft section at Wal-Mart. Your other option is to just carefully fold it so that the print is inside your sterile foil package, then fold the edges over a time or 2 to seal it up. A little piece of tape won't hurt either.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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MushroomWizard420
mycological magician



Registered: 04/03/14
Posts: 307
Loc: space
Last seen: 1 year, 9 months
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Re: Best spore printing tek [Re: 36fuckin5]
#19954577 - 05/07/14 03:26 PM (9 years, 8 months ago) |
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And in swoops 36fuckin5 with some excellent tips and a condescending tone, securing the win for Team Perfect! 
seriously though, good tips 365. i didn't know that bit about alcohol, and you summed up the important parts well. helpful as always..
-------------------- "And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good." -Shroomesis 4:20  MushroomWizard420 The spirit of the forest watches over his children as they grow...
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Quote:
MushroomWizard420 said: And in swoops 36fuckin5 with some excellent tips and a condescending tone, securing the win for Team Perfect! 
seriously though, good tips 365. i didn't know that bit about alcohol, and you summed up the important parts well. helpful as always.. 
Haha, I never claimed to not be an asshole. I just claim that if I open my mouth I know what I'm talking about.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 6 months
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Re: Best spore printing tek [Re: 36fuckin5]
#19954599 - 05/07/14 03:32 PM (9 years, 8 months ago) |
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I like that idea of using a whole sheet and just cutting up later. 36 mafia wut wut.
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Pineal Crust
Feeling psilly.



Registered: 11/27/13
Posts: 205
Last seen: 1 year, 10 months
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Re: Best spore printing tek [Re: Mdahmer]
#19954755 - 05/07/14 04:21 PM (9 years, 8 months ago) |
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I use a 6qt shoebox sized tub instead of a SAB, very good info!
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MushroomWizard420
mycological magician



Registered: 04/03/14
Posts: 307
Loc: space
Last seen: 1 year, 9 months
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Re: Best spore printing tek [Re: 36fuckin5]
#19954808 - 05/07/14 04:34 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said:
Quote:
MushroomWizard420 said: And in swoops 36fuckin5 with some excellent tips and a condescending tone, securing the win for Team Perfect! 
seriously though, good tips 365. i didn't know that bit about alcohol, and you summed up the important parts well. helpful as always.. 
Haha, I never claimed to not be an asshole. I just claim that if I open my mouth I know what I'm talking about.
I think that comment actually sums up your personality entirely. and i mean that as a compliment lol. you've helped me a lot with posts and shit, and i envy your ability to be as blunt as feel like being whenever you want. brutal honesty can hurt while it helps
-------------------- "And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good." -Shroomesis 4:20  MushroomWizard420 The spirit of the forest watches over his children as they grow...
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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The 6 qt box doesn't help anything. When you go to bag or fold them up they're gonna get fucked because they're exposed to open air. You NEED a SAB to make clean prints. No ways around it. I wouldn't even suggest a flowhood for this.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Pineal Crust
Feeling psilly.



Registered: 11/27/13
Posts: 205
Last seen: 1 year, 10 months
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Re: Best spore printing tek [Re: 36fuckin5]
#19954838 - 05/07/14 04:42 PM (9 years, 8 months ago) |
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I understand its importance now, thanks
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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I seen a tek that I've been trying out with a little variation. Ive been pre-cutting my foil pieces to a reasonable size. Then pre-folding them and unfolding them to get the creases where I want. I then place all these in a bigger piece of foil and bake in the oven at 350 for like an hour. I then take the whole package still hot straight to the SAB unfold and take them out and put them on the rack.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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That's a waste of time. Foil is already sterile.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19954991 - 05/07/14 05:18 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: Using alcohol-wiped scissors, cut the cap off as close to the gills as you can get without touching them.
Aren't you risking transferring bacteria from the scissors to the stem here? Flame sterilize a scalpel ?? They are cheap enough at a grow store $2.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: invitro]
#19955056 - 05/07/14 05:32 PM (9 years, 8 months ago) |
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Scalpels suck for cutting caps. And alcohol-wiped scissors are much cleaner than the open air you fruit in.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Beside the Garden


Registered: 06/03/13
Posts: 606
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Re: Best spore printing tek [Re: invitro]
#19955058 - 05/07/14 05:32 PM (9 years, 8 months ago) |
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Ok I read on here months ago...
Use your sterilization box with the arm holes cut in it. Have a few zip lock baggies in there, make sure every thing is a steril as posable. Put a fresh cap into the baggie and let it sit. After some time has passed and you get a good print remove the cap and seal it up. When you need the print inject your solution into the baggie, shake then suck it backup into the syringe. inoculate.
Anybody see any flaws in this or why it couldn't work. Never done it myself but it sounded pretty simple and clean.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19955070 - 05/07/14 05:34 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: Scalpels suck for cutting caps. And alcohol-wiped scissors are much cleaner than the open air you fruit in.
flame sterilize the scissors then? 1 bacteria in a print is one too many.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: invitro]
#19955083 - 05/07/14 05:36 PM (9 years, 8 months ago) |
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Quote:
Beside the Garden said: Ok I read on here months ago...
Use your sterilization box with the arm holes cut in it. Have a few zip lock baggies in there, make sure every thing is a steril as posable. Put a fresh cap into the baggie and let it sit. After some time has passed and you get a good print remove the cap and seal it up. When you need the print inject your solution into the baggie, shake then suck it backup into the syringe. inoculate.
Anybody see any flaws in this or why it couldn't work. Never done it myself but it sounded pretty simple and clean.
It doesn't allow you to use agar. Fuck making spore syringes.
Quote:
invitro said: flame sterilize the scissors then? 1 bacteria in a print is one too many.
You're not gonna ever get a 100% clean print no matter what you do. Maybe if you fruit invitro and keep it in front of a flowhood the entire time.
Again, the scissors are cleaner than your fruiting conditions.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19955098 - 05/07/14 05:38 PM (9 years, 8 months ago) |
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Op didn't ask for perfect tek he asked for best tek. Best tek is flame sterilizing the scissors.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: invitro]
#19955128 - 05/07/14 05:43 PM (9 years, 8 months ago) |
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No, it's fucking not.
You really need to stop spreading bad info. I'm tired of chasing you around and fixing it.
Flame sterilizing your scissors is gonna melt the fucking handle.
I posted the way the pros do it. I've done it many, many times myself. Nobody has ever complained about my prints.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19955144 - 05/07/14 05:47 PM (9 years, 8 months ago) |
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Metal scissors then, bacterial contams in a print is seriously bad. Put me on iggy if I'm too much for you to handle.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: invitro]
#19955158 - 05/07/14 05:50 PM (9 years, 8 months ago) |
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No, then you'll just spread your bullshit and I won't be able to correct it. You'll get a bunch of noobs with contaminated shit that will post and I'll have to answer them.
You know not what you speak of. Your print is 99% sure to ALREADY have some bacteria in it. Go get a vendor print or syringe and put it under a microscope.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19955183 - 05/07/14 05:54 PM (9 years, 8 months ago) |
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Your logic: because there are already some bacteria, it's ok to add more, even when there is a perfectly good alternative to not add more. And you should do it this way, "because the pros" do it this way. Did I get you right there?
Edited by invitro (05/07/14 05:54 PM)
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capti
Stranger


Registered: 11/01/13
Posts: 81
Last seen: 15 days, 16 hours
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Re: Best spore printing tek [Re: invitro]
#19955237 - 05/07/14 06:03 PM (9 years, 8 months ago) |
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if you were going to sterilize scissors using a flame would be a bad idea you could cook it in the oven at a low temp or maybe pressure cook it .... if for some reason alcohol was not enuff for you
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Best spore printing tek [Re: capti]
#19955246 - 05/07/14 06:05 PM (9 years, 8 months ago) |
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Quote:
capti said: if you were going to sterilize scissors using a flame would be a bad idea you could cook it in the oven at a low temp or maybe pressure cook it .... if for some reason alcohol was not enuff for you
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: capti]
#19955248 - 05/07/14 06:05 PM (9 years, 8 months ago) |
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No, my logic is that flaming your scissors is just a waste of fucking time. Alcohol works plenty well enough.
Make shit harder on yourself if you want.
I really think you're just arguing to argue at this point.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5] 1
#19955286 - 05/07/14 06:13 PM (9 years, 8 months ago) |
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365 said at the beginning of the post:
"Third, wiping foil (which is already sterile) with alcohol is like washing your face then wiping shit on it. Alcohol doesn't sterilize anything, it only sanitizes. Big difference."
So wiping the foil with alcohol is like wiping shit on your face, but it's ok to wipe your scissors with alcohol and get that shit on the the stem and then onto the foil. Thanks for clearing that up. Your best move now would be to concede the point gracefully because everyone can see your wrong, sorry.
I'm not arguing to argue, I'm arguing to keep nubs from screwing up all their prints.
Edited by invitro (05/07/14 06:21 PM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: invitro]
#19955321 - 05/07/14 06:20 PM (9 years, 8 months ago) |
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The difference is that your fruit was never sterile to begin with. The foil was. What we can't flame, we wipe with alcohol. You can't flame scissors because the handle will melt, or you'll burn yourself on the handle. Scalpel blades are super thin and cool quickly.
I'm not gonna concede and say I'm wrong because I'm not. I'm just getting tired of being the only TC around besides when RR wants to pop in. You're obviously not gonna believe me, so I need somebody who actually knows what the fuck they're talking about to come help me shut you up.
Get off the forums and go read on microbiology from someone with a degree. scholar.google.com is your friend.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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capti
Stranger


Registered: 11/01/13
Posts: 81
Last seen: 15 days, 16 hours
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Re: Best spore printing tek [Re: invitro]
#19955325 - 05/07/14 06:21 PM (9 years, 8 months ago) |
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the foil is sterile its rolled at temps that keep it sterile unless you unroll it and are waving it around
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Dr.Dankhead
Uhh...doctor gonzo?



Registered: 03/29/13
Posts: 5,187
Loc: Breathing down your neck
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Quote:
liamtheloser said: small closed room, properly sterilized. wipe a sheet of foil with alcohol to sterlize it, place the caps on the foil, then cover each cap with a sterilized (with alcohol) shot glass (or drinking glass, w/e)
wait 24 hours, re-sterilize the small closed room, wear sterile gloves, pick up the shot glass, carefully remove cap, fold foil over the top of the print, fold the edges in so it's a sealed little square, then do that for every print you're taking.
That's way overkill..
I print mine on sterile foil, open room, no hardcore sterilization Needed..
Never have i had a syringe not work..
The syringe tek is what's TRUELY important.. I'm lazy as shit with prints and it always works
--------------------
**need a check up?** **im a Doctor**
         i sometimes wish I was a wormy, wiggling all in the cold dirt...tickle tackle pickle dickle think a mackshift thought of broken words broken gears and words of conundrums..I'm not a weiner doctor so take that shit to dr. Gonz free boob inplant consultations.. Photo required
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Quote:
Dr.Dankhead said:
Quote:
liamtheloser said: small closed room, properly sterilized. wipe a sheet of foil with alcohol to sterlize it, place the caps on the foil, then cover each cap with a sterilized (with alcohol) shot glass (or drinking glass, w/e)
wait 24 hours, re-sterilize the small closed room, wear sterile gloves, pick up the shot glass, carefully remove cap, fold foil over the top of the print, fold the edges in so it's a sealed little square, then do that for every print you're taking.
That's way overkill..
I print mine on sterile foil, open room, no hardcore sterilization Needed..
Never have i had a syringe not work..
The syringe tek is what's TRUELY important.. I'm lazy as shit with prints and it always works
You're just lucky. That doesn't work for most people. It's all important to keep as clean as you can.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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MushroomWizard420
mycological magician



Registered: 04/03/14
Posts: 307
Loc: space
Last seen: 1 year, 9 months
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Re: Best spore printing tek [Re: 36fuckin5]
#19955358 - 05/07/14 06:28 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: I'm not gonna concede and say I'm wrong because I'm not. I'm just getting tired of being the only TC around besides when RR wants to pop in. You're obviously not gonna believe me, so I need somebody who actually knows what the fuck they're talking about to come help me shut you up.
I will be your Messiah and carry this burden.
-------------------- "And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good." -Shroomesis 4:20  MushroomWizard420 The spirit of the forest watches over his children as they grow...
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19955376 - 05/07/14 06:30 PM (9 years, 8 months ago) |
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Your logic is still essentially: because the stem is not sterile it's ok to add more contams to it. I'm saying that anyone with any sense can see this is not a smart move. Bring in all the extra people you need. Your aim shouldn't be to shut me up, but to find the truth and educate.
How can you say it's all important to keep it is as clean as you can and then not sterilize your scissors. You don't need to get metal scissors so hot it burns your hand, wear thick rubber gloves is one solution, with the capped hooked on a safety clip cutting with a scalpel should be no problem.
Does anyone else see what I'm saying here? One second he says cleanliness is all important the next he says it's ok to bypass the easy procedure of flame sterilizing the tool to keep the print clean. It's not that hard.
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Beside the Garden


Registered: 06/03/13
Posts: 606
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Re: Best spore printing tek [Re: 36fuckin5]
#19955398 - 05/07/14 06:34 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said:
Quote:
Beside the Garden said: Ok I read on here months ago...
Use your sterilization box with the arm holes cut in it. Have a few zip lock baggies in there, make sure every thing is a steril as posable. Put a fresh cap into the baggie and let it sit. After some time has passed and you get a good print remove the cap and seal it up. When you need the print inject your solution into the baggie, shake then suck it backup into the syringe. inoculate.
Anybody see any flaws in this or why it couldn't work. Never done it myself but it sounded pretty simple and clean.
It doesn't allow you to use agar. Fuck making spore syringes.
Ok so could using sterilized cotton swabs (the q-tip ones for lab use) to transfer the spore from the zip lock to agar work?
Doing this all in the glove box. use alcohol to clean the out side of the zip lock first cracking the edge of the bag open so minimal air gets in dip the cotton swab in immediately transfer.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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If you wanted to do that, an inoculation loop would be better. But just having it on regular foil is better than that.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: 36fuckin5]
#19955422 - 05/07/14 06:37 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: Get off the forums and go read on microbiology from someone with a degree. scholar.google.com is your friend.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Beside the Garden


Registered: 06/03/13
Posts: 606
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Re: Best spore printing tek [Re: 36fuckin5]
#19955439 - 05/07/14 06:39 PM (9 years, 8 months ago) |
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Cool, Thanks.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5]
#19955445 - 05/07/14 06:40 PM (9 years, 8 months ago) |
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I cannot imagine any kind of article that is going to back up your position, help me out with some keywords to search for or something!
I don't take much at face value, neither do you. I can't see why you find my questions so irritating, I'm basically you without as much experience. You can't just say stuff without backing up why and expect everyone to believe you then get all pissed off when they want some proof. As it stands your position looks completely unreasonable to the average person, you realize that right?
Edited by invitro (05/07/14 06:50 PM)
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MushroomWizard420
mycological magician



Registered: 04/03/14
Posts: 307
Loc: space
Last seen: 1 year, 9 months
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Re: Best spore printing tek [Re: invitro]
#19955502 - 05/07/14 06:51 PM (9 years, 8 months ago) |
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Quote:
invitro said: I cannot imagine any kind of article that is going to back up your position, help me out with some keywords to search for or something!
I don't take much at face value, neither do you. I can't see why you find my questions so irritating, I'm basically you without as much experience.
here are some keywords to get you started: "stop arguing i'm sure your way works but 365 has eons of experience, so even if you don't like his answers/explanations i'm sure he has reasons for them."
you can both be my mushroom princesses if you guys stop clogging this thread with butthurt
-------------------- "And on the last day, God purchased RogerRabbit's DVD set, and learned to grow mushrooms. And it was good." -Shroomesis 4:20  MushroomWizard420 The spirit of the forest watches over his children as they grow...
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Well there is not one shread of butthurt in my posts, and from where I stand he is giving seriously bad advice that will mess up prints for a lot of nubs.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: 36fuckin5]
#19955510 - 05/07/14 06:53 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: The difference is that your fruit was never sterile to begin with. The foil was. What we can't flame, we wipe with alcohol. You can't flame scissors because the handle will melt, or you'll burn yourself on the handle. Scalpel blades are super thin and cool quickly.
^^^This
We fruit in open air. Which means making syringes out of prints fruited in open air is a risky proposition. It can be done, does not mean it should be done. We also spray out hands down with iso before SAB work. Its not perfect but I personally don't feel like flame sterilizing my hands 
Unless your using a invitro tek like Violets, you should treat prints as if they are dirty. I have never picked up a contam on a plate I streaked with a print I took myself, but I would still always make at least one transfer before inoculation to ensure a clean culture.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: Pastywhyte]
#19955519 - 05/07/14 06:55 PM (9 years, 8 months ago) |
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Quote:
Pastywhyte said:
Quote:
36fuckin5 said: The difference is that your fruit was never sterile to begin with. The foil was. What we can't flame, we wipe with alcohol. You can't flame scissors because the handle will melt, or you'll burn yourself on the handle. Scalpel blades are super thin and cool quickly.
^^^This
We fruit in open air. Which means making syringes out of prints fruited in open air is a risky proposition. It can be done, does not mean it should be done. We also spray out hands down with iso before SAB work. Its not perfect but I personally don't feel like flame sterilizing my hands 
Unless your using a invitro tek like Violets, you should treat prints as if they are dirty. I have never picked up a contam on a plate I streaked with a print I took myself, but I would still always make at least one transfer before inoculation to ensure a clean culture.
So your saying, because it's not 100% clean it's ok to make the stem dirty whichs sit directly in the center of the print, risking the cleanliness of the print, risking the whole grow? You want to talk about extra work? How much extra work is it going to be to clean up those bacteria laden agar plates your about to make with that extra dirty print you just made??
Edited by invitro (05/07/14 06:57 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: invitro]
#19955530 - 05/07/14 06:58 PM (9 years, 8 months ago) |
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I'm saying that if you use spore prints you take yourself, to use agar Syringes are crap anyway. I did 12 cakes and said fuck it, I'm doing agar.
If you don't take your spores from the area where the stem was (shouldn't be any spores there anyway) and use a tiny amount, it will work out quite well 99% of the time.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5] 1
#19955540 - 05/07/14 06:59 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said:
Quote:
36fuckin5 said: Get off the forums and go read on microbiology from someone with a degree. scholar.google.com is your friend.
Done - found zero support for your position, anything else?
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: invitro]
#19955542 - 05/07/14 06:59 PM (9 years, 8 months ago) |
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I just bite the stipe off with my teeth, haven't had a single contam'd print yet
-------------------- LAGM v 2.024 - endo cabendo
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: Pastywhyte] 1
#19955560 - 05/07/14 07:03 PM (9 years, 8 months ago) |
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Quote:
Pastywhyte said: I'm saying that if you use spore prints you take yourself, to use agar Syringes are crap anyway. I did 12 cakes and said fuck it, I'm doing agar.
If you don't take your spores from the area where the stem was (shouldn't be any spores there anyway) and use a tiny amount, it will work out quite well 99% of the time.
I'm saying use agar with a print that has half the bacteria as the ones 365 makes, that way you waste less agar plates. Syringes are only as crappy as the vendor you get them from. taking spores from a certain area is your sure-fire solution? You don't think that bacteria in the middle of the print can waft over to any other part of the print with the slightest air-current or bump from the cultivator? How about when you fold the print, you don't that junk is getting everywhere? How about not being lazy and flaming the scalpel or what have you?
Edited by invitro (05/07/14 07:03 PM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Best spore printing tek [Re: invitro]
#19955612 - 05/07/14 07:12 PM (9 years, 8 months ago) |
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How about you go fucking try it yourself before shooting shit down. This thread will be hidden now.
Like I said, make shit harder on yourself if you want. Just stop touting it as fact when you've obviously never tried it. And stop telling all these noobs things as fact when you really don't know what you're talking about.
My way does NOT add bacteria. You just don't understand sterility as well as you think you do. I've made easily thousands of prints. When I do any prints, I generally fill my SAB to the point where I only have a tiny little space to work in. Easily 100 at a time. They're all pretty much clean.
What you think you know from reading and what happens in real-world environments are 2 different things. Why do you think NOBODY EVER says to sterilize scissors.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: invitro] 1
#19955638 - 05/07/14 07:16 PM (9 years, 8 months ago) |
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Bacteria is not airborne in most cases and if you allow your print to dry properly that will kill most bacteria. Like I said, I have not yet had a print I took contam once streaked to agar, but I still treat it carefully. Personally I don't relish the idea of flaming my scissors 25-50 times when making prints.
Besides it won't matter if you flame sterilize or not, when you cut the stipe you are pushing any contams on the stipe itself across the area you just cut
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: 36fuckin5] 1
#19955739 - 05/07/14 07:35 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: How about you go fucking try it yourself before shooting shit down. This thread will be hidden now.
Like I said, make shit harder on yourself if you want. Just stop touting it as fact when you've obviously never tried it. And stop telling all these noobs things as fact when you really don't know what you're talking about.
My way does NOT add bacteria. You just don't understand sterility as well as you think you do. I've made easily thousands of prints. When I do any prints, I generally fill my SAB to the point where I only have a tiny little space to work in. Easily 100 at a time. They're all pretty much clean.
What you think you know from reading and what happens in real-world environments are 2 different things. Why do you think NOBODY EVER says to sterilize scissors.
All you had to say was that experience has proven you right despite what it seems like. Getting all angry and throwing curse words around, that's something you might meditate on or get some counseling for cuz it didn't convince anyone of anything on this side and it's probably not helping you much either.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: Pastywhyte]
#19955781 - 05/07/14 07:45 PM (9 years, 8 months ago) |
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Quote:
Pastywhyte said: Bacteria is not airborne in most cases and if you allow your print to dry properly that will kill most bacteria. Like I said, I have not yet had a print I took contam once streaked to agar, but I still treat it carefully. Personally I don't relish the idea of flaming my scissors 25-50 times when making prints.
Besides it won't matter if you flame sterilize or not, when you cut the stipe you are pushing any contams on the stipe itself across the area you just cut 
Your still arguing by the logic that if it's a little dirty it's ok to make it more dirty. This is poor logic and you should abandon it completely. Bacteria is everywhere, and in the air in serious numbers, open a petri plate to the air and watch the bacteria grow. When that bacterial laden scissor blade hits the stem, and that all dries out, it's not too hard to see it dropping down right into the print, the center, the sides etc.
My point once again is, flaming the scalpel is easy, it definitely reduces contams of all kinds not just bacteria. It's not ok to make a print dirtier because it's dirty already. It's pretty cut and dry, if 365 gets away with it ok, but like muda said, why cut corners if you don't have to? Maybe after a bunch of prints I will realize it won't make a difference.
Edited by invitro (05/07/14 07:57 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: Pastywhyte]
#19955849 - 05/07/14 07:59 PM (9 years, 8 months ago) |
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Quote:
invitro said: Your still arguing by the logic that if it's a little dirty it's ok to make it more dirty.
Not at all. I'm arguing that the contams that you should be worried about are already present on the stipe and will be there whether you flame sterilize or not. The fact is that because the fruit was in open air, there are contams all around the stipe, falling down, regardless of what you cut it with. Short of suspending the cap over the print (and I've seen people do this) which would not be foolproof anyway, you are not going to prevent the odd contam from the side of the stipe from getting onto the print. So your flame sterilization is not contributing to a clean print in any meaningful way.
Quote:
Pastywhyte said: Besides it won't matter if you flame sterilize or not, when you cut the stipe you are pushing any contams on the stipe itself across the area you just cut 
I am not one to normally cut corners but try making a few hundred prints, then use them and you will understand what I am talking about. There is a reason that every decent cultivator when discussing spore inoculations stresses "small amounts" regardless of whether talking about spore syringes, inoculating pf jars, inoculating agar, etc. We know its a crapshoot. But this time its in our favor. Fact is that with my method, I have yet to see a contam show up on a plate inoculated with one of my prints. I have seen far more on prints received from sponsors.
You really want to have a challenge, try using wild prints. Then you will know what cleaning a dirty print is all about.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: Pastywhyte]
#19955874 - 05/07/14 08:05 PM (9 years, 8 months ago) |
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Quote:
Pastywhyte said:
Quote:
invitro said: Your still arguing by the logic that if it's a little dirty it's ok to make it more dirty.
Fact is that with my method, I have yet to see a contam show up on a plate inoculated with one of my prints. I have seen far more on prints received from sponsors.
That statement hit the nail right on the head, that's all 365 needed to say to me. I can accept that until such time as I test it out myself. Way to keep your cool Pasty and thanks for the advice.
I don't care about rank or title or bla bla, I just want to hear results, I'm a pretty easy going if I can get some real results.
Edited by invitro (05/07/14 08:10 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: invitro]
#19955897 - 05/07/14 08:10 PM (9 years, 8 months ago) |
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No worries man when you test it out be sure to post pics of the subsequent grow
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: invitro]
#19955901 - 05/07/14 08:10 PM (9 years, 8 months ago) |
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Pasty, if you keep responding like that, you'll never get your "Boss Ass Bitch" tag
-------------------- LAGM v 2.024 - endo cabendo
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Meh, as long as I can still be . . .
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: Pastywhyte]
#19955929 - 05/07/14 08:17 PM (9 years, 8 months ago) |
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nope, there's only 360 degrees in a one man circle-jerk...soooo 360 + 5... nope, no room for any more I'm afraid 
edit: SHUT YOUR WHORE MOUTH AND LISTEN TO MY WORDS!
-------------------- LAGM v 2.024 - endo cabendo
Edited by MonkeyJesusFresco (05/07/14 08:18 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: Pastywhyte]
#19955944 - 05/07/14 08:21 PM (9 years, 8 months ago) |
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-------------------- LAGM v 2.024 - endo cabendo
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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wtf is going on in this thread
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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as an aside, i always wondered how vendors do sterile spore syringes. that must be really hard, and i wonder if they test batches to any degree? and how often.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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I would think microscope, if i were a vendor, I would definetly microscopy a syringe or two at random...ya know...microscopy and all
-------------------- LAGM v 2.024 - endo cabendo
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Fuck I wanna know how the fuck they make APE syringes. Can't print em. Can't see if its sterile.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: Pastywhyte]
#19956046 - 05/07/14 08:39 PM (9 years, 8 months ago) |
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-------------------- LAGM v 2.024 - endo cabendo
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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and apparently there's such a thing as "spore swab"...(every-fuck'n-day, something new, I swear )
http://www.shroomery.org/forums/showflat.php/Number/15617015
-------------------- LAGM v 2.024 - endo cabendo
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 7 days, 20 hours
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I've just been using a new snack sized zip-lock baggie. Reach in my SGFC, place baggie over top of cap, then snip it off with flame sterilized scissors. I then seal the bag, shake the cap into the corner, let sit up to 24hrs, bottom of cap down. Open bag upside down in a SAB, reseal bag, but leave sides in bottom apart so the print dries. This may not be the best way by far. However, I've started spawn from several prints taken this way & have not had a problem so far. I might get flamed for posting this procedure. (It's also a tek on here) but hey, it's worked great so far. And super simple.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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seriously, compare that procedure and its results to the "standard" methods of spore printing and see...ya might be going through a lot of trouble for nothing 
the first method for spore printing I ever tried and still do to this day, is the "standard" still air box methods described further up in this thread, and was super impressed at the clean cultures that came from it (also, didn't use a still air box, so that might play a big roll in me being soooo impressed)
-------------------- LAGM v 2.024 - endo cabendo
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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believe it or not, even after 9 months of growing, i JUST did my first ever print a few days ago. I've not tested it yet. i usually just went back to the first couple prints/syringes i had if i was not doing clones.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Quote:
MonkeyJesusFresco said: and apparently there's such a thing as "spore swab"...(every-fuck'n-day, something new, I swear )
http://www.shroomery.org/forums/showflat.php/Number/15617015
Do you PC the q-tips??
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: Testertips]
#19956232 - 05/07/14 09:20 PM (9 years, 8 months ago) |
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yeah, I was hung up on tissue culturing for awhile myself, if you're using agar, you will not be disapoint!
Quote:
Testertips said:
Quote:
MonkeyJesusFresco said: and apparently there's such a thing as "spore swab"...(every-fuck'n-day, something new, I swear )
http://www.shroomery.org/forums/showflat.php/Number/15617015
Do you PC the q-tips?? 
nah, flame sterilize
-------------------- LAGM v 2.024 - endo cabendo
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
Pastywhyte said: Fuck I wanna know how the fuck they make APE syringes. Can't print em. Can't see if its sterile. 
i asked once, apperently they just shoot water at the gills lol
Quote:
MonkeyJesusFresco said: well, here's an idea 
http://www.shroomery.org/forums/showflat.php/Number/14085370
i'm sure pasty knows this lol, i'm guessing his question is in more regards to where do vendors get spore syringes from a mushroom that doesn't like to drop spores
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: cronicr]
#19956621 - 05/07/14 10:32 PM (9 years, 8 months ago) |
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Jot



Registered: 10/25/13
Posts: 1,194
Loc: East of the Cascades, Wa
Last seen: 4 years, 3 months
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Re: Best spore printing tek [Re: invitro]
#19956768 - 05/07/14 11:10 PM (9 years, 8 months ago) |
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Quote:
invitro said: I'm basically you
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: Jot]
#19956801 - 05/07/14 11:22 PM (9 years, 8 months ago) |
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that comment was in response to an 11 year old Trusted Cultivator, who has little tact and an abundance of impatience for "noobs" and their ideas;
the idea behind the comment is that, for the most part, a good majority of us start at a relatively similar level of knowledge and work are way up;
invitro was insinuating that 36fuckin5 (the 11 year old) is just like him but older, as the search function will verify;
thus, it was a plea of compassion.
hope that helps!
-------------------- LAGM v 2.024 - endo cabendo
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Jot



Registered: 10/25/13
Posts: 1,194
Loc: East of the Cascades, Wa
Last seen: 4 years, 3 months
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I can't find anything saying that 36fuckin5 is 11YO Do you mean he was a member for 11 years?
-------------------- The goal of spiritual life is not altered states, but altered traits
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Best spore printing tek [Re: Jot]
#19958485 - 05/08/14 11:18 AM (9 years, 8 months ago) |
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yes a member for 11 years
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Jot



Registered: 10/25/13
Posts: 1,194
Loc: East of the Cascades, Wa
Last seen: 4 years, 3 months
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Re: Best spore printing tek [Re: cronicr]
#19959115 - 05/08/14 01:39 PM (9 years, 8 months ago) |
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Impressive!
-------------------- The goal of spiritual life is not altered states, but altered traits
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: Jot]
#19965447 - 05/09/14 09:24 PM (9 years, 8 months ago) |
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365 I can see how you might think I am just being argumentative, but here is a quick explanation just incase this issue is still bothering you. When I knew nothing I tried other people's teks without really thinking about it too much. I ran into some very serious setbacks, taking even the most trusted advice from even the most trusted cultivators. I live in a different part of the world (the tropics) than most people and that is one reason why some thing just don't work for me so everything has to go though hell and high water now before I can accept it. I give zero credit for teks that are uber popular before I test them because some of them seriously will fail me and I'm not about to let that happen again.
I think that if you want the best you've got to put it all to the test, it's my take on things right now and it's not likely to change. Hope that helps.
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trophycase
Dickface

Registered: 03/23/11
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Re: Best spore printing tek [Re: 36fuckin5] 1
#19965641 - 05/09/14 10:29 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: You NEED a SAB to make clean prints. No ways around it. I wouldn't even suggest a flowhood for this.
Sorry but this just isn't true. You can print them in individual jars. Take a rectangle of foil, fold it to a 90 degree angle, put inside a jar; repeat for as many prints as you want to take. Then sterilize your jars. After your jars cool you pretty much do the process you explained... I used thumb tacks to stick into the caps, transfer to the foil, close the jar. Then when your print is finished just clean your area well, fold the foil over the print (that's why rectangle of foil. Cutting a big sheet of prints just always seemed unnecessary to me. Takes a lot more time.) and bag it up. Essentially the jars are individual SABs for each cap. But that's just one way to do it without the SAB. I have done it tons of times with fantastic results.
-------------------- All of the cultivation photos uploaded by this account were taken in an area of the globe where such practices are legal and uninhibited.
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bahamutdoom
Vulcan


Registered: 03/10/14
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Re: Best spore printing tek [Re: trophycase] 1
#19965708 - 05/09/14 10:53 PM (9 years, 8 months ago) |
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Why couldn't you sterilize the jars, and print directly onto the bottom of the jar? This way you can simply remove the cap, fill jar with water, and pull syringes from that? This would be less steps, thus creating less chances of contamination. As long as it was done properly goal achieved right?
Would that work anyone? Ive never made a print but seem logical to me lol
-------------------- Common sense is not so common...
Edited by bahamutdoom (05/09/14 10:59 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
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it works, your gonna wanna do some on foil though if ya ever wanna trade people
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Making syringes from prints is risky but at least you can scrape from parts of the print that are going to have a better shot of being clean. Printing in a jar where you inject water will ensure that any contams that were present make it inside the syringe.
Fact is that when working with prints your best bet is to use agar.
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Inocuole
Scalpel of Evil's Bane




Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Best spore printing tek [Re: Pastywhyte]
#19965871 - 05/09/14 11:56 PM (9 years, 8 months ago) |
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Tell me this, fellow douche-nozzles.
Amidst all this talk of putting prints to agar, if you have a mushroom, and some agar, and a scalpel, why are you not just taking tissue clones?
I can only see spores being preferable if you have senescence kicking in or you need to re-roll your genetics for whatever reason.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
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Re: Best spore printing tek [Re: Inocuole]
#19965880 - 05/09/14 11:59 PM (9 years, 8 months ago) |
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many reasons for spore prints...what if you loose your master culture?
-------------------- LAGM v 2.024 - endo cabendo
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Inocuole
Scalpel of Evil's Bane




Registered: 11/21/11
Posts: 24,863
Loc: ★
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Is there a reason other than that? That's about the only one that makes sense that I can think of, other than what I mentioned previously. Wouldn't just continually doing grain transfers ensure that you always have a master jar?
I know spore prints can last for years, I guess that's one upside.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
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Re: Best spore printing tek [Re: invitro]
#19965924 - 05/10/14 12:16 AM (9 years, 8 months ago) |
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For trading purposes! and storage! and it's just good mycology really, plus they're so small, noth'n to it!
-------------------- LAGM v 2.024 - endo cabendo
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: invitro]
#19965929 - 05/10/14 12:19 AM (9 years, 8 months ago) |
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sidenote!
Quote:
invitro said: 365 I can see how you might think I am just being argumentative, but here is a quick explanation just incase this issue is still bothering you. When I knew nothing I tried other people's teks without really thinking about it too much. I ran into some very serious setbacks, taking even the most trusted advice from even the most trusted cultivators. I live in a different part of the world (the tropics) than most people and that is one reason why some thing just don't work for me so everything has to go though hell and high water now before I can accept it. I give zero credit for teks that are uber popular before I test them because some of them seriously will fail me and I'm not about to let that happen again.
I think that if you want the best you've got to put it all to the test, it's my take on things right now and it's not likely to change. Hope that helps.
I think 36 has hidden this thread, so...fuck I guess I'll devil-advocate...
Quote:
The Devil says: If you knew nothing then how do you know you did other people's teks correctly...blah...Living in the tropics has nothing to do with your failures blah blah blah it's your technique and lack of experience blah blah. Teks are uber popular for a reason blah blah we do things a certain way because they work blah blah blah...
how'd I do?
-------------------- LAGM v 2.024 - endo cabendo
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: Inocuole]
#19967184 - 05/10/14 10:46 AM (9 years, 8 months ago) |
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Quote:
Inocuole said: Tell me this, fellow douche-nozzles.
Amidst all this talk of putting prints to agar, if you have a mushroom, and some agar, and a scalpel, why are you not just taking tissue clones?
I can only see spores being preferable if you have senescence kicking in or you need to re-roll your genetics for whatever reason.
Not all clones perform the same. IME I will take 10 clones and end up keeping one. Once I have a decent cloned culture in a master slant, I still want to try and top it so I do isolations from spores in the hope of finding that one in 1000 strain.
Quote:
Inocuole said: Is there a reason other than that? That's about the only one that makes sense that I can think of, other than what I mentioned previously. Wouldn't just continually doing grain transfers ensure that you always have a master jar?
I know spore prints can last for years, I guess that's one upside.
You can only G2G about 5 times before the culture degrades. You would use a master slant to keep tissue going. Prints are good because they preserve the varity, can be traded, and allow you to search for specific traits. They are also the begining of any grow
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Inocuole
Scalpel of Evil's Bane




Registered: 11/21/11
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Re: Best spore printing tek [Re: Pastywhyte]
#19967227 - 05/10/14 11:01 AM (9 years, 8 months ago) |
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Is a culture with many different genetics any less susceptible to senescence or degradation? Like, suppose you do absolutely no isolation, straight from spores. That's still going to last just as long as an isolated strain for transfers? Just trying to get information more than anything, I've grown for years and still never taken a spore print, didn't seem worthwhile until I started asking direct questions about it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: Inocuole]
#19967269 - 05/10/14 11:12 AM (9 years, 8 months ago) |
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Quote:
Inocuole said: Is a culture with many different genetics any less susceptible to senescence or degradation? Like, suppose you do absolutely no isolation, straight from spores. That's still going to last just as long as an isolated strain for transfers? Just trying to get information more than anything, I've grown for years and still never taken a spore print, didn't seem worthwhile until I started asking direct questions about it.
Pretty much the same. There may be some minute differences but nothing that is going to allow you to preserve the genetics indefinitely. Plus the amount of expansion in grains is far greater than what you would see on a plate so there are more cell divisions working against you.
For people working with agar, spores are all about having the ability to select from an infinite gene pool. Usually when I get a new varity/species to play with my route goes; ms grow, clone, isolate, test isolates, keep best one. Then once I have time to waste and not worried about dud genetics then I will work on isolating from spores in the search for something truly unique or awesome.
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Inocuole
Scalpel of Evil's Bane




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Posts: 24,863
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Re: Best spore printing tek [Re: Pastywhyte]
#19967293 - 05/10/14 11:17 AM (9 years, 8 months ago) |
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Would you say that keeping a very small master grain jar vs transferring from one batch to the next would help preserve the genetics just slightly longer? It makes sense that that would help decrease the spore divisions taking place in the culture that I'm pulling from. I think I have everything I need for spore prints, I'm just not that confident with it yet. I have the still air box, have some agar plates that I dropped colonized grains on, but don't really want to have to get more spores later on in the case of degradation.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Best spore printing tek [Re: Inocuole]
#19967326 - 05/10/14 11:26 AM (9 years, 8 months ago) |
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While you could keep a culture alive in a grain master for quite some time, its a rather clumsy and bulky way to do it. The grain culture will get very hard to break up after more than a couple months in the fridge, and if you just try to G2G to keep the culture going, you will be expanding it a lot more than agar transfers. The best way to preserve a culture is a master slant, which is very small, lasts for a year or two before a transfer is needed, and with proper media rotation can keep your culture viable for decades.
If you just want inoculate and don't care about isolated genetics, a print makes more sense. Prints are very small, and last a long time. Its easy to make a prints, just be sure to use agar for your first inoculation. Agar is forgiving and will save you from any problems your print making tek might have caused.
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Inocuole
Scalpel of Evil's Bane




Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Best spore printing tek [Re: Pastywhyte]
#19967379 - 05/10/14 11:41 AM (9 years, 8 months ago) |
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Sweet, that's pretty much what I got from the rest of the thread but there was a whole bunch of drama queen shit in here so I figured I'd clarify, perhaps add to the conversation. Thanks.
Also lol@tropics causing teks to fail.
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mezzy jeb
Skywalker


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Re: Best spore printing tek [Re: 36fuckin5]
#20218615 - 07/03/14 08:55 AM (9 years, 6 months ago) |
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Quote:
36fuckin5 said: What you need to do is make a SAB. Take your roll of foil in there (after wiping the outside with alcohol) and rip off the first foot or so. Throw that away, it's touched open air and is dirty.
Not to be a smartass, but this doesn't make sense. You're recommending bringing dirty tinfoil into your SAB. Doesn't that just spread contaminates around?
I thought the idea of a SAB was to get everything inside, and everything wiped down with alcohol and then bringing nothing dirty in except sanitized gloved hands and flame sterilized stuff.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 hours, 14 minutes
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Re: Best spore printing tek [Re: mezzy jeb]
#20218649 - 07/03/14 09:06 AM (9 years, 6 months ago) |
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no, the idead behind a Still Air Box is to keep the air still, so contams don't fall in; the only reason I/we blast SABs with lysol is to make ourselves feel better does no real good. you do need sterile tools, however. gloved hands keep your nasty skin-flakes/contams from get'n in your work. on the other hand, a moist spraying of something like distilled water or water:bleach, there is a point there, the moisture settles and traps lots of contaminates that you could possibly "stir up" while working.
foil comes off a fresh roll sterile (the process for making foil is HOT one )
edit:
but don't get me wrong! If ya wanna spray/wipe/blow-torch suface areas and what not in your SAB, by all means do it, even if it's technically just making you feel better. No shit, I take the rings off my grain jars and blast around 'em with lysol. dun't know if it does anything, but it makes me feel better before handling them
Edited by MonkeyJesusFresco (07/03/14 09:09 AM)
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