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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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as an aside, i always wondered how vendors do sterile spore syringes. that must be really hard, and i wonder if they test batches to any degree? and how often.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 12 hours, 22 minutes
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I would think microscope, if i were a vendor, I would definetly microscopy a syringe or two at random...ya know...microscopy and all
-------------------- LAGM v 2.024 - endo cabendo
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Fuck I wanna know how the fuck they make APE syringes. Can't print em. Can't see if its sterile.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 12 hours, 22 minutes
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Re: Best spore printing tek [Re: Pastywhyte]
#19956046 - 05/07/14 08:39 PM (9 years, 8 months ago) |
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-------------------- LAGM v 2.024 - endo cabendo
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 12 hours, 22 minutes
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and apparently there's such a thing as "spore swab"...(every-fuck'n-day, something new, I swear )
http://www.shroomery.org/forums/showflat.php/Number/15617015
-------------------- LAGM v 2.024 - endo cabendo
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 3 minutes
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I've just been using a new snack sized zip-lock baggie. Reach in my SGFC, place baggie over top of cap, then snip it off with flame sterilized scissors. I then seal the bag, shake the cap into the corner, let sit up to 24hrs, bottom of cap down. Open bag upside down in a SAB, reseal bag, but leave sides in bottom apart so the print dries. This may not be the best way by far. However, I've started spawn from several prints taken this way & have not had a problem so far. I might get flamed for posting this procedure. (It's also a tek on here) but hey, it's worked great so far. And super simple.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 12 hours, 22 minutes
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seriously, compare that procedure and its results to the "standard" methods of spore printing and see...ya might be going through a lot of trouble for nothing 
the first method for spore printing I ever tried and still do to this day, is the "standard" still air box methods described further up in this thread, and was super impressed at the clean cultures that came from it (also, didn't use a still air box, so that might play a big roll in me being soooo impressed)
-------------------- LAGM v 2.024 - endo cabendo
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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believe it or not, even after 9 months of growing, i JUST did my first ever print a few days ago. I've not tested it yet. i usually just went back to the first couple prints/syringes i had if i was not doing clones.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Testertips
all good


Registered: 03/09/14
Posts: 679
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Quote:
MonkeyJesusFresco said: and apparently there's such a thing as "spore swab"...(every-fuck'n-day, something new, I swear )
http://www.shroomery.org/forums/showflat.php/Number/15617015
Do you PC the q-tips??
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 12 hours, 22 minutes
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Re: Best spore printing tek [Re: Testertips]
#19956232 - 05/07/14 09:20 PM (9 years, 8 months ago) |
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yeah, I was hung up on tissue culturing for awhile myself, if you're using agar, you will not be disapoint!
Quote:
Testertips said:
Quote:
MonkeyJesusFresco said: and apparently there's such a thing as "spore swab"...(every-fuck'n-day, something new, I swear )
http://www.shroomery.org/forums/showflat.php/Number/15617015
Do you PC the q-tips?? 
nah, flame sterilize
-------------------- LAGM v 2.024 - endo cabendo
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
Pastywhyte said: Fuck I wanna know how the fuck they make APE syringes. Can't print em. Can't see if its sterile. 
i asked once, apperently they just shoot water at the gills lol
Quote:
MonkeyJesusFresco said: well, here's an idea 
http://www.shroomery.org/forums/showflat.php/Number/14085370
i'm sure pasty knows this lol, i'm guessing his question is in more regards to where do vendors get spore syringes from a mushroom that doesn't like to drop spores
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Best spore printing tek [Re: cronicr]
#19956621 - 05/07/14 10:32 PM (9 years, 8 months ago) |
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Jot



Registered: 10/25/13
Posts: 1,194
Loc: East of the Cascades, Wa
Last seen: 4 years, 3 months
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Re: Best spore printing tek [Re: invitro]
#19956768 - 05/07/14 11:10 PM (9 years, 8 months ago) |
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Quote:
invitro said: I'm basically you
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 12 hours, 22 minutes
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Re: Best spore printing tek [Re: Jot]
#19956801 - 05/07/14 11:22 PM (9 years, 8 months ago) |
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that comment was in response to an 11 year old Trusted Cultivator, who has little tact and an abundance of impatience for "noobs" and their ideas;
the idea behind the comment is that, for the most part, a good majority of us start at a relatively similar level of knowledge and work are way up;
invitro was insinuating that 36fuckin5 (the 11 year old) is just like him but older, as the search function will verify;
thus, it was a plea of compassion.
hope that helps!
-------------------- LAGM v 2.024 - endo cabendo
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Jot



Registered: 10/25/13
Posts: 1,194
Loc: East of the Cascades, Wa
Last seen: 4 years, 3 months
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I can't find anything saying that 36fuckin5 is 11YO Do you mean he was a member for 11 years?
-------------------- The goal of spiritual life is not altered states, but altered traits
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Best spore printing tek [Re: Jot]
#19958485 - 05/08/14 11:18 AM (9 years, 8 months ago) |
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yes a member for 11 years
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Jot



Registered: 10/25/13
Posts: 1,194
Loc: East of the Cascades, Wa
Last seen: 4 years, 3 months
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Re: Best spore printing tek [Re: cronicr]
#19959115 - 05/08/14 01:39 PM (9 years, 8 months ago) |
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Impressive!
-------------------- The goal of spiritual life is not altered states, but altered traits
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Best spore printing tek [Re: Jot]
#19965447 - 05/09/14 09:24 PM (9 years, 8 months ago) |
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365 I can see how you might think I am just being argumentative, but here is a quick explanation just incase this issue is still bothering you. When I knew nothing I tried other people's teks without really thinking about it too much. I ran into some very serious setbacks, taking even the most trusted advice from even the most trusted cultivators. I live in a different part of the world (the tropics) than most people and that is one reason why some thing just don't work for me so everything has to go though hell and high water now before I can accept it. I give zero credit for teks that are uber popular before I test them because some of them seriously will fail me and I'm not about to let that happen again.
I think that if you want the best you've got to put it all to the test, it's my take on things right now and it's not likely to change. Hope that helps.
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trophycase
Dickface

Registered: 03/23/11
Posts: 1,018
Last seen: 5 years, 8 months
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Re: Best spore printing tek [Re: 36fuckin5] 1
#19965641 - 05/09/14 10:29 PM (9 years, 8 months ago) |
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Quote:
36fuckin5 said: You NEED a SAB to make clean prints. No ways around it. I wouldn't even suggest a flowhood for this.
Sorry but this just isn't true. You can print them in individual jars. Take a rectangle of foil, fold it to a 90 degree angle, put inside a jar; repeat for as many prints as you want to take. Then sterilize your jars. After your jars cool you pretty much do the process you explained... I used thumb tacks to stick into the caps, transfer to the foil, close the jar. Then when your print is finished just clean your area well, fold the foil over the print (that's why rectangle of foil. Cutting a big sheet of prints just always seemed unnecessary to me. Takes a lot more time.) and bag it up. Essentially the jars are individual SABs for each cap. But that's just one way to do it without the SAB. I have done it tons of times with fantastic results.
-------------------- All of the cultivation photos uploaded by this account were taken in an area of the globe where such practices are legal and uninhibited.
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bahamutdoom
Vulcan


Registered: 03/10/14
Posts: 31
Last seen: 4 years, 10 months
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Re: Best spore printing tek [Re: trophycase] 1
#19965708 - 05/09/14 10:53 PM (9 years, 8 months ago) |
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Why couldn't you sterilize the jars, and print directly onto the bottom of the jar? This way you can simply remove the cap, fill jar with water, and pull syringes from that? This would be less steps, thus creating less chances of contamination. As long as it was done properly goal achieved right?
Would that work anyone? Ive never made a print but seem logical to me lol
-------------------- Common sense is not so common...
Edited by bahamutdoom (05/09/14 10:59 PM)
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